Information
A simple, scalable and rapid method for sequencing up to 576 SARS-CoV-2 samples:
- Midnight Expansion Kit with RT-PCR reagents and primers to amplify SARS-CoV-2 from input RNA
- Rapid Barcoding Kits 96 V14 to barcode and prepare the amplified material for sequencing
- MinION/GridION Flow Cells to perform sequencing on MinION or GridION devices
- Sequence for as little as $12.65 per sample
How it works
The Midnight method amplifies the SARS-CoV-2 genome in 29 overlapping amplicons (~1,200 bp long). By amplifying longer amplicons, Oxford Nanopores rapid chemistry can be deployed enabling users to rapidly attach a barcode, pool samples and adapt them for sequencing. This method is simple and quick to deploy. It allows rapid turn-around-times that can assist with monitoring of novel variants of concern.
The protocol delivers robust full genome coverage across a wide range of Ct values.
Users can pair the library preparation with an Oxford Nanopore analysis solution to enable a complete end-to-end workflow.
The Midnight bundle includes everything you need to sequence SARS-CoV-2:
| Feature | Midnight bundle |
|---|
| Preparation time | 5 h 30 min including RT-PCR |
| Hands-on time | 1 h 15 min |
| Input amount | 8 μl extracted RNA |
| Multiplexing | run in sets of 24* - 96 samples per flow cell |
| Pack size | 576 samples |
| Storage | Shipped at 2-8°C
Flow cells: long-term storage 2-8°C
Kits: long-term storage -20°C |
| Compatibility | MinION Mk1B
MinION Mk1C
GridION Mk1
Please contact us for PromethION |
| 3rd party reagents required | Ethanol, nuclease-free water |
*users wishing to run in multiples of 24 will need to buy 3 x Flow Cell Wash Kits (EXP-WSH004).
Shipping and logistics:
Flow cells and kits are shipped together in an environmentally friendly temperature-controlled shipping box.
Products are shipped to customers within the USA and EU Monday to Thursday. Shipments to Canada, Norway, Korea and Japan are expedited Monday to Wednesday; with Australia and New Zealand leaving our warehouses on a Friday. Shipments to the rest of the world are made on Mondays to allow the full working week for packages to arrive.
The delivery charges are calculated when a quote is raised or during checkout. Once an order is made, the delivery ID and delivery information