Vaccinia Capping System - 400 units

Vaccinia Capping System provides the necessary components to add 7-methylguanylate cap structures (Cap 0) to the 5' end of RNA. In eukaryotes, these terminal cap structures are involved in stabilization, transport, and translation of mRNAs. Enzymatic production of capped RNA is an easy way to improve the stability and translational competence of RNA used for in vitro translation, transfection, and microinjection. Alternatively, use of labeled GTP in a reaction provides a convenient way to label any RNA containing a 5' terminal triphosphate. Vaccinia capping enzyme is composed of two subunits (D1 and D12). The D1 subunit carries three enzymatic activities (RNA triphosphatase, guanylyltransferase and guanine methyltransferase); all necessary for addition of a complete Cap 0 structure, m7Gppp5'N to 5' triphosphate RNA. In vitro transcripts can be capped in less than one hour in the presence of the capping enzyme, reaction buffer, GTP, and the methyl donor, SAM.