NEBNext® Library Quant Kit for Ultima Genomics® - 100 reactions

The NEBNext Library Quant Kit for Ultima Genomics delivers precise and reliable qPCR-based quantification of NGS libraries prepared using the UG 100 SolarisTM Free workflow. The kit features forward and reverse primers targeting the Ultima adaptor sequence, and includes five high-quality, pre-diluted DNA standards for reliable quantitation of diluted DNA libraries. Additionally, it incorporates the Luna Universal qPCR Master Mix with a blue, non-interfering, visible tracking dye, which can also serve as a passive reference dye, eliminating the need for additional ROX in real-time instruments requiring ROX normalization. The streamlined, user-friendly protocol minimizes pipetting steps and uses a single extension time for all libraries, enhancing efficiency while generating reproducible, high-quality results. Library quant values can be calculated using NEB’s online tool: NEBioCalculator.