Quick Blunting and Quick Ligation Kits - 20 reactions

NEB offers the Quick Blunting and Quick Ligation Kits together as a bundle at a value price. The Quick Blunting Kit is used to convert DNA with incompatible 5' or 3' overhangs to 5' phosphorylated, blunt-ended DNA for efficient blunt-end ligation into DNA cloning vectors. DNA is blunted using T4 DNA polymerase which has both 3' to 5' exonuclease activity and 5' to 3' polymerase activity. T4 Polynucleotide Kinase is included in the enzyme mix for phosphorylation of the 5' ends of blunt-ended DNA for subsequent ligation into a cloning vector. This kit is optimized for blunting up to 5 µg of DNA in a single reaction. The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature (25C).