Properties
| Related Categories | Biochemicals and Reagents, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors, |
| trade name | Chemicon |
| brand family | Chemicon |
| application(s) | Cell Culture |
| | Stem Cell Culture |
| stem cell type | Neural Stem Cells |
| | Epithelial Cells |
| | Mesenchymal Stem Cells |
| specific activity | Chemicon's Human FGF-b is fully biologically active when compared to standards. The ED50 was determined by the dose-dependent stimulation of thymidine uptake by NIH3T3 cells expressing FGF receptors. |
| purity | Greater than 95% by SDS-PAGE. Endotoxin level is less than 0.1 EU per µg of FGF-b. |
| source | expressed in E. coli |
| species | Human |
| NCBI accession no. | NM_002006.4 |
| UniProt accession no. | P09038 |
| gene symbol | FGF2(2247) |
| | BFGF |
| | FGFB |
| | HBGF-2 |
| | PROSTATROPIN |
| material size | 50 µg |
| shipped in | dry ice |
| storage conditions | Maintain the lyophilized material at -20°C until expiration date as stated on the label.
General applications:
After a quick spin, reconstitute in 0.1M phosphate buffer, pH 6.8, to a concentration of 0.1-1.0 mg/mL. Reconstituted bFGF should be stored in working aliquots at -20 °C for up to six months. Multiple freeze/thaw cycles will result in significant loss of activity.
For Human ES cell culture:
After a quick spin, reconstitute to 10 µg/mL in a filtered solution of 0.5% BSA, 1 mM DTT, and 10% glycerol in Dulbecco's PBS. Aliquot and store at -20°C for up to six months. This solution can then thawed and diluted to 4 ng/mL for the culture of human ES cells with a feeder layer, or to 8 ng/mL to supplement mouse embryonic fibroblast-conditioned medium (for feeder-free human ES cell culture). |
Other Notes
Fibroblast Growth Factor-basic (FGF-b) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-b also exerts a potent angiogenic activity in vivo. Human FGF-b is a 17.2 kDa protein containing 155 amino acid residues.
Background information
Fibroblast Growth Factor-basic (bFGF) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. bFGF also exerts a potent angiogenic activity in vivo. Human bFGF is a 17.2 kDa protein containing 155 amino acid residues. Xu, et al. demonstrated that bFGF synergizes with the BMP antagonist noggin to sustain undifferentiated proliferation of human embryonic stem (hES) cells under feeder-free conditions. GF003-AF was developed without animal-based ingredients and can be used for the culture of hES cells in a feeder-free, animal-free culture system.
Physical form
Lyophilized from a 0.7 mg/mL solution in 5mM Tris, pH 7.6 with 150 mM NaCl.
Application
For most in vitro applications, bFGF exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL. Responding cells are (partial list): Endothelial, mesenchymal cells. Human ES cells require concentrations in the range of 4 to 100 ng/mL, depending on the method of culture.
Optimal working dilutions must be determined by end user.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.