Properties
| Related Categories | Amber Glass Bottles, Analytical Reagents, Analytical/Chromatography, Biochemicals and Reagents, Chromatography Reagents & Solvents, |
| grade | for HPLC, gradient grade |
| vapor density | 1.11 (vs air) |
| vapor pressure | 410 mmHg ( 50 °C) |
| | 97.68 mmHg ( 20 °C) |
| InChI Key | OKKJLVBELUTLKV-UHFFFAOYSA-N |
| assay | ≥99.9% |
| autoignition temp. | 725 °F |
| expl. lim. | 36 % |
| impurities | KMnO4 red. matter (as O), in accordance |
| | ≤0.0005% free alkali (as NH3) |
| | ≤0.0005% non-volatile matter |
| | ≤0.001% acetaldehyde |
| | ≤0.001% acetone (GC) |
| | ≤0.001% formaldehyde |
| | ≤0.02% water (Karl Fischer) |
| | ≤1 ppb fluorescence (quinine) at 254 nm |
| | ≤1 ppb fluorescence (quinine) at 365nm |
| evapn. residue | <0.0003% |
| color | APHA: ≤10 |
| refractive index | n20/D 1.329(lit.) |
| bp | 64.7 °C(lit.) |
| mp | −98 °C(lit.) |
| density | 0.791 g/mL at 25 °C(lit.) |
| absorption | HPLC-gradient/230 nm ≤2 mAU |
| | HPLC-gradient/254 nm ≤5 mAU |
| | absorption/ in accordance |
| UV absorption | λ: 210 nm Amax: 0.50 |
| | λ: 220 nm Amax: 0.30 |
| | λ: 230 nm Amax: 0.15 |
| | λ: 235 nm Amax: 0.10 |
| | λ: 240 nm Amax: 0.05 |
| | λ: 260 nm Amax: 0.01 |
| | λ: 400 nm Amax: 0.01 |
Other Notes
Pure-Pac® II containers require the Micromatic MacroValve coupler for dispensing solvents, Z560723.
Packaging
1 pkg in Pure-Pac™ 2
1, 6×1, 2, 4, 4×4 L in glass bottle
100 mL in glass bottle
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Preparation Note
Product filtered through a 0.2 μm filter
Application
Methanol (MeOH) has been used as an eluent in the HPLC estimation of malondialdehyde in plasma, which is an indicator of oxidative stress.
It may be used in the following studies:
• Colony forming unit-fibroblast assay of bone marrow mononuclear cells.
• As solvent for the preparation of extracts of hyphae of Aspergillus for the estimation of gliotoxin by reversed phase-HPLC.
• Immunofluorescence studies.
• To compose eluent for the ion-pair reverse-phase HPLC isolation of nucleotides and their decomposition products.
General description
Thermochemical conversion of methanol to C2-C10 hydrocarbons in the presence of shape-selective zeolites has been reported. Its oxidation on Ru-Pt catalyst system by ruthenium ad-atoms has been proposed.
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Legal Information
Pure-Pac is a registered trademark of Sigma-Aldrich Co. LLC