Properties
| Related Categories | Antibodies, Biochemicals and Reagents, Biotin/Avidin, Supplementary Products |
| brand family | Calbiochem |
| packaging | 1 mg in Plastic ampoule (189730-1MG) |
| | 10 mg in Plastic ampoule (189730-10MG) |
| form | Lyophilized solid |
| specific activity | =10 units/mg protein |
| toxicity | Standard Handling |
| shipped in | ambient |
| storage temp. | -20C |
| storage conditions | Protect from moisture |
| | Ok to freeze |
Overview
A high affinity biotin binding protein (Kd approximately 10-15) that is stable over wide pH and temperature ranges. Each molecule of streptavidin binds 4 molecules of biotin. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point nearer to neutrality (pI of 5.5-6.5 versus 10 for avidin). Consequently, streptavidin frequently exhibits lower levels of non-specific binding than avidin. The extinction coefficient of a 1% solution at 280 nm is 32.
A high-affinity biotin binding protein.
Unit Definition
One unit is defined as the amount of streptavidin required to bind 1.0 µg of D-Biotin at pH 7.0.
Storage and Shipping Instructions
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Aqueous stock solutions are stable for 6 months at 4°C and for up to 2 years at -20°C.