Amersham ECL Gold Hybridization Buffer sufficient for 4000 cm² ECL Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 2NA min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRPcatalyzed breakdown of luminol.