Amersham ECL Plex Goat-a-Rabbit IgG-Cy5, for 1000 cm² membrane area, 150yg
Broad dynamic range
High sensitivity
Ability to detect multiple proteins on same blot (multiplexing)
Wide pH tolerance (3 to 10), making them compatible with most Western blotting buffers
High photostability
Signal longevity > 3 months
Fluorescence detection is a direct Western blotting method in which a secondary antibody is conjugated to a fluorophore, avoiding the need for ancillary detection reagents.
Amersham Cy3 and Cy5 secondary antibodies are labeled with nearinfrared (NIR) fluorophores that emit light at wavelengths of 570 and 670 nm. By using Amersham Cy3 and Cy5 secondary antibodies together with a pair of appropriate rabbit or mouse primary antibodies, you can perform multiplexed experiments.