Cypher 5E NHS Ester, 5mg
Generic technology can be coupled to any receptor or epitope tag antibody.
Monitors any neutral to acidic event.
Simple fluorescence intensity increase.
Classic CyDye profile of two heterocycles linked by a polymethine bridge, and contains secondary nitrogen atoms in one or both heterocyclic rings.
CypHer5E has additional sulfonate groups to enhance its solubility in aqueous solutions.
Increased labelling efficiency and reduces adverse dyedye interactions.
CypHer5E is a redexcitable, pHsensitive cyanine dye derivative that is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH. It is therefore well suited to report the movement of a receptor from the cell surface into acidic endosomes upon agonist stimulation.