Sera-Xtracta Genomic DNA Kit
Suitable for longread sequencing: Isolates highmolecularweight DNA > 200 kb.
Efficient scaleup and flexibility: Provides robust chemistry for sample input volumes from 200 µL – 2 mL.
Efficient removal of inhibitors to give high purity genomic DNA: Typical purity ratio (A260/A280 and A260/A230) of > 1.7.
Automation friendly reagents and easy to use protocols
Simplified protocol minimizes the copurification of RNA.
Compatible with molecular biology techniques, including nextgeneration sequencing (NGS), cloning, restriction enzyme digestion, PCR amplification and genotyping applications.
SeraXtracta Genomic DNA Kit offers magnetic bead based extraction and purification of genomic DNA from whole blood. The simplified protocol minimizes the copurification of RNA, removing the need for RNAse treatment in most applications. The kit yields genomic DNA with the high purity and quality required by today’s molecular biology techniques.
The kit uses chaotropic agents to extract DNA from blood cells, denature protein components and promote the selective binding of DNA to the silicacoated magnetic beads. Proteinase K is the protease of choice to digest protein from samples, because it is active even when enzyme inhibitors such as EDTA and detergents are present in samples. Denatured contaminants are easily removed by subsequent washing of the silica beads with an ethanolic buffer set. The purified genomic DNA is eluted in a low ionic strength buffer at a concentration suitable for most downstream molecular biology applications.