HiTrap Desalting, 1 x 5 mlL columns with Sephadex G-25 resinConvenient desalting and buffer exchange of sample volumes up to 1.5 mL.
Two to five columns can be connected in series for sample sizes from 3 mL to 7.5 mL.
Replaces dialysis for desalting protein, which saves time and reduces labor.
Requires less than one minute with typically > 95% recovery.
HiTrap column format is convenient for use with a syringe, a peristaltic pump, or a chromatography system.
HiTrap Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction