HiPrep Phenyl HP 16/10, 20 mL
Aromatic HIC medium for intermediate purification and polishing.
Selectivity similar to Phenyl Sepharose 6 Fast Flow (Low Sub) but higher resolution due to the smaller 34 µm bead size.
Provides fast, easy highcapacity separations in a convenient format.
Phenyl Sepharose HP chromatography medium is based on a matrix of highly crosslinked agarose beads, which have been derivatized with phenyl groups. These are attached to the agarose matrix via uncharged, chemically stable ether linkages. The result is a truly hydrophobic medium with no ionic properties.
High mechanical strength of the matrix allows for high flow rates, together with good resolution. Capacities vary according to the protein applied and its concentration. Loading capacity will also vary depending on the salt concentration, pH, and temperature at which the protein is applied.