Vivaspin 20 MWCO 3000, 12/pk
Easeofuse: Onestep sample concentration in a single tube, for minimal sample handling and reduced sample loss, as well as pH compatibility over a broad range – pH 1 to 9.
Sample protection: Patented Vivaspin deadstop technology ensures samples cannot be concentrated to dryness and enables direct concentrate recovery.
Easeofuse: Onestep sample concentration in a single tube, for minimal sample handling and reduced sample loss.
High flow rates: And minimal membrane blockage through the vertical polyethersulfone (PES) membrane design.
Minimal protein adsorption: Thanks to the Vivaspin™ polycarbonate tube design.
Wide selection: Vivaspin sample concentrators are suitable for sample volumes ranging from 100 µL to 20 mL, with a range of molecular weight cutoff (MWCO) values from Mr 3000 to 100 000.
Vivaspin™ ultrafiltration spin columns are designed for fast, nondenaturing concentration and desalting of proteins and other biological samples. the target molecule typically exceeding 95%. The entire process is performed in a single tube with an upper compartment containing sample and lower compartment separated by a semipermeable membrane with a molecular weight cutoff (MWCO) selected by the user. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber.
Spin column protein concentration – how does it work?
Membrane spin columns such as Vivaspin combine the principle of size exclusion with centrifugation to achieve rapid concentration and high recovery of target biomolecules in a single tube. An upper compartment containing sample is separated from the lower compartment by a semipermeable membrane with an appropriate MWCO value chosen to exclude molecules of interest. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber. The Vivaspin column design permits protocols for onestep biomolecule concentration, rapid sample recovery (reverse spin protocol), desalting, and buffer exchange.
How to select the right spin column for your applicationSample volume and molecular weight of the target biomolecule(s) are the primary factors to consider when choosing a spin column. Vivaspin offers a choice of membranes to cover different ultrafiltration requirements (Table 1).
Tip: For maximum recovery, select a MWCO value at least 50% smaller than the molecular size of the species of interest.Applications for ultrafiltration spin columnsVivaspin ultrafiltration spin columns are a rapid and flexible solution for protein concentration and smallscale preparation of biological molecules directly from cell lysates and biological fluids, following upstream purification steps. They can also be used prior to downstream analyses, such as gel electrophoresis, liquid chromatography (LC), mass spectrometry (MS), and LCMS. Examples include:
Concentration of urine prior to electrophoresis for identification and diagnosis of multiple myeloma and related serum protein disorders.
Concentration of recombinant human IFNg following purification from culture supernatant.