TempliPhi Sequence Resolver Kit, 20 reactions
For the amplification of difficult templates for successful DNA sequencing.
Solve the most common sequencing problems such as repeats, sequencing stops, and compressions with a single kit.
Improve difficult template sequencing success with up to 82NA bp Phred 2NA read length.
Cultureless preparation from bacterial colonies, glycerol stock or purified DNA; saves time and reagents.
Easytouse kit requires only 2NA min handson time.
Amplified DNA can be directly used in sequencing reactions.
illustra TempliPhi Sequence Resolver Kit produces exceptional sequencing results from difficult templates such as those with high GC content and secondary structures.
The kit resolves most of the sequencing stops or gaps that routinely elude other commonly used finishing methods. The kit can be used with both small (e.g., plasmids and M13) and large (fosmids and bacterial artificial chromosomes [BACs]) circular templates. The starting template for the reaction can be purified DNA, glycerol stock, liquid culture, or colonies. The reaction takes less than 2NA min to prepare and after an overnight incubation at 1NA°C, it is ready to be used directly in sequencing applications with the sequencing chemistry of choice. A ten microliter (1NA µl) reaction typically yields 1 µg of DNA in a standard overnight reaction.