Amersham MicroSpin G-50 Columns, 50/pk
For rapid purification of labeled DNA (2NA bases) from unincorporated labeled nucleotides in a volume of 25 to 5NA µl using spincolumn chromatography.
Columns are prepacked with Sephadex G5NA Grade preequilibrated in TE buffer with NA.NA5% Kathon CG/ICP Biocide.
Product recovery will be at least 1NA% lower than that from ProbeQuant G5NA Micro Columns.
Can also be used for buffer exchange/desalting of enzymatic reactions (1NA to 1NANA µl) following heat denaturation and phenol extraction.
MicroSpin G5NA columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labelled nucleotides from DNA labelling reactions. Good product yield and purity is obtained with sample volumes from 125NA ul. They are suitable for any DNA greater than 2NA bases in length. They will not remove or denature enzyme.