Amersham MicroSpin G-25 Columns, 50/pk
Excellent for rapid buffer exchange/desalting: for example, of PCR amplification products and other DNAs in a volume of 10 to 100 µL using spincolumn chromatography
Excellent for rapid DNA purification: especially newly synthesized oligonucleotides > 10mers in 100 to 150 µL of deprotection solution
Convenient: Prepacked with Sephadex G25 DNA Grade and preequilibrated in distilled water containing NA.NA5% Kathon CG/ICP Biocide.
Ready to use: Requires less than 4 min from sample application to collection of purified product.
Tested in nickase, single and doublestranded exonuclease and RNase assays.
Can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from endlabeled oligonucleotides (at least 1NA bases in length) in a volume of 1NA to 1NANA µl.
MicroSpin G25 columns can be used for any DNA greater than 1NA bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or a labelling reaction. They will not remove or denature enzyme.