PreScission Protease for GST-tag removal, 500 units
Specific cleavage – between the Gln and Gly residues of the recognition sequence LeuGluValLeuPheGln/GlyPro.
Timesaving – GSTtagged proteins can be cleaved while still bound to an affinity resin.
Low incubation temperature – enables low temperature cleavage of fusion proteins containing the HRV 3C protease recognition sequence.
GST tags and protease can be removed in a single step while fusion proteins are still bound to Glutathione Sepharose 4B, Glutathione Sepharose High Performance, or Glutathione Sepharose 4 Fast Flow affinity resin. Another option is to elute the GSTtagged protein using reduced glutathione, cleave, then pass the sample over the same resin to remove the protease and tags.
Vectors encoding the HRV 3C protease recognition sequence
pGEX6P vectors pGEX6P1, pGEX6P2, and pGEX6P3 encode the recognition sequence for this 3C protease.