Amersham Hot Start Master Mix, 100 reactions
Recombinant hot start activator protein sequesters primers prior to PCR, effectively reducing nonspecific priming and primerdimer formation.
Quick hot start activation preserves sample integrity by avoiding extensive precycling heat treatment.
Avoids the risk of contamination from a mammalian source associated with hot start antibodies.
Convenient, readytouse mix saves time and reduces experimental variability.
Confidence in downstream applications; increased read length in sequencing applications.
For any standard PCR reaction, such as multiplex PCR, and reactions exhibiting nonspecific amplification or primerdimer formation.
illustra Hot Start Master Mix uses a novel hot start method called primer sequestration.
Illustra Hot Start Master Mix combines highquality recombinant Taq DNA Polymerase, a recombinant hot start activator protein, and nucleotides in a proprietary reaction buffer. This readytouse mix provides robust and reliable performance for demanding PCR applications in which high specificity and high sensitivity are essential to success. The mix does not contain Taq antibody and this eliminates the risk of contamination from a mammalian source. Also, since the polymerase is not chemically inactivated, there is no extensive precycling heating step necessary; this reduces the chance of damaging precious DNA samples from heatinduced depurination. The net result from using Illustra Hot Start Master Mix is a more robust and reliable amplification of the target amplicons with increased yield when compared to conventional hot start master mix products.