Immobiline DryStrip Gels, pH 3-11 NL, 18 cm
Maximize resolution, loading capacity, and reproducibility of firstdimension isoelectric focusing (IEF).
Choose from a comprehensive range of overlapping Immobiline DryStrip gels that cover narrow (1 pH unit), medium (3–5 pH units), and broad (8 pH units) pH ranges.
Select the broad pH 3–11 NL gradient Immobiline DryStrip gel for fast and efficient screening to gain a broad overview of total protein distribution.
For more detailed studies or when using greater protein loads, choose from four new overlapping strips covering the pH 3 to 11 range.
Higher resolution can be obtained using narrowrange (1 pH unit) Immobiline DryStrip gels.
Use matching IPG buffers for optimized running conditions.
Use IPG strips directly after reswelling without further manipulation.
Barcoded strips for convenient sample and batch tracking.
Immobiline DryStrip gels are available in fourteen overlapping pH ranges and five strip lengths (7, 11, 13, 18, and 24 cm) and represent the most comprehensive selection of narrow, medium, and widerange IPG strips available. The five narrow pH range (1 pH unit), 24cm IPG strips deliver the highest resolution and protein loading capacity available, which results in well separated spots with high protein content after the seconddimension separation.
The pH 3–11 NL strip covers most proteins found in prokaryotic and eukaryotic cells while the narrowrange strips can be used when higher resolution is required. The pH gradients at the extreme ends of the pH scale are nonlinear to distribute the proteins evenly over the gel length to obtain maximal resolution.
Immobiline DryStrip gels are rehydrated with a matching IPG Buffer prior to use. Samples can be included directly in the rehydration solution for efficient loading of dilute samples or for loading larger amounts of protein. Samples can also be loaded after rehydration. The firstdimension separation is carried out using Ettan IPGphor 3 IEF system or Multiphor II flatbed system.