HiScreen Capto Core 700
For intermediate purification and polishing of viruses and other large biomolecules (Mr 7NANA NANANA) in flowthrough mode.
Novel core bead technology allows efficient capture of contaminants while target molecules are collected in the flowthrough.
Significantly improved productivity and higher flow rates compared with gel filtration methods.
Straightforward optimization due to flowthrough chromatography and robust performance.
Convenient smallscale purification, process development, and scaleup using prepacked HiTrap and HiScreen columns.
Capto Core 7NANA is composed of a ligandactivated core and inactive shell. The inactive shell excludes large molecules (cut off ~ Mr 7NANA NANANA [7NANA kDa]) from entering the core through the pores of the shell. These larger molecules are collected in the column flowthrough while smaller impurities bind to the internalized ligands. The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core. The multimodal ligands ensure strong binding with most impurities over a wide range of pH and salt concentrations. Bound impurities are removed from the beads by cleaninginplace (CIP) procedures using NaOH and in most cases, a solvent.
Capto Core 7NANA is available in various pack sizes for packing columns and in convenient, prepacked 1 ml HiTrap and 4.7 ml prepacked HiScreen formats. HiTrap and HiScreen columns can be connected in series for increased capacity and are convenient to use when developing an efficient and robust separation method.