Ni Sepharose 6 Fast Flow, 100 mL

by Cytiva


  • 17531802 | NI SEPHAROSE 6FF, 100 ML
  • 17531802 | NI SEPHAROSE 6FF, 100 ML
  • 17531802 | NI SEPHAROSE 6FF, 100 ML
  • 17531802 | NI SEPHAROSE 6FF, 100 ML
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Product Description

Ni Sepharose 6 Fast Flow histidine-tagged protein purification resin, 100 mL

Designed for scaling up purification of histidinetagged proteins

High protein binding capacity and minimal leakage of Ni2? ions

As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.

Available in convenient prepacked HisPrep and HisTrap column formats.

Excellent flow properties provide great performance even at high flow rates.

Ni Sepharose 6 Fast Flow is a member of the GE Healthcare range of IMAC chromatography media for capture and intermediate purification.

Ni Sepharose 6 Fast Flow is composed of crosslinked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix and precharged with Ni2? ions for purification of histidine tagged proteins.

The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity. Ni Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient prepacked formats for easy scaleup and process development. As member of the BioProcess media range, Ni Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support

Specifications
Inventory Number:
GEH17531802EA
Part Number:
17531802
Supplier:
Cytiva
Supplier's Lead Time:
Call for Lead Time
Quantity:
1 per EA
Contract Availability:
GSA
Country of Origin:
SWEDEN
Binding Capacity/ml Chromatography Medium:
Approx. 40 mg histidine-tagged protein
BioProcess resin:
yes
Chemical Stability:
Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea
Chromatography technique:
Histidine-tagged protein purification
Licensing:
For licensing information, see the Licensing Statements page in the About Us section.
Matrix:
6% cross-linked agarose
Metal Ion Capacity:
~15 µmol Ni2+/ml medium
Pack size:
100 mL
Particle Size:
45 µm-165 µm
Particle size, d50V:
~90 µm
pH stability, CIP:
2–14
pH stability, operational:
3–12
Pressure/Flow Specification:
Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
Storage:
4 to 30°C, 20% Ethanol
Additional Information

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