GSTrap™ HP, 5 x 1 mL
High resolution purification of GSTtagged proteins.
Mild elution conditions preserving protein antigenicity and function.
Fast, onestep purification.
Easy to use with syringe, pump or a chromatography system.
GSTrap HP 1ml and 5ml columns are prepacked with Glutathione Sepharose High Performance medium, which provides highresolution purifications, thus a more concentrated sample. GSTtagged proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose High Performance. GSTtagged proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer
GSTtagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a sitespecific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GSTtagged proteins can be detected using colorimetric or immunological methods.