HiTrap ANX Sepharose FF (High Sub), 16 mm
Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
The industry standard for ion exchange chromatography.
High flow rates and good scaleup potential.
Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
Predictable scaleup
Q, SP, DEAE, and CM Sepharose Fast Flow are based on a robust, 6% highly crosslinked beaded agarose matrix with good flow properties and high loading capacities. ANX Sepharose 4 Fast Flow (high sub) is based on 4% highly crosslinked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.
The active end of the charged group is the same for DEAE Sepharose Fast Flow and ANX Sepharose Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose Fast Flow has a diethylaminoethylgroup bound to the agarose whilst ANX Sepharose 4 Fast Flow (high sub) has a diethylaminopropyl group attached.