Ni Sepharose excel, 100 mL
Versatile – suitable for eukaryotic samples, including insect and CHO cells, as well as bacterial samples such as E. coli.
Direct sample load – of eukaryotic cell culture supernatants, eliminating the need for pretreatment of samples that usually cause nickel stripping.
Retained binding capacity of up to 10 mg/mL resin.
Simplified sample handling – fewer steps needed compared with conventional workflow.
Flexible formats – prepacked and loose resin formats for optimized screening and preparative histag purification.
Ni Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24 h incubation in 10 mM EDTA.
Ni Sepharose excel enables direct loading of samples without having to perform extensive and timeconsuming pretreatment procedures. The flow properties of the medium make it excellent for purifications in a wide range of scales and allow loading of large sample volumes, enabling purification of low concentrations of target proteins at large volumes.