rProtein A Sepharose Fast Flow antibody purification resin, 5 mL
For monoclonal and polyclonal antibody purification at both laboratory and process scale.
Wellestablished Protein A medium used in many approved MAb processes
Free from animalderived components.
Hydrophilic base matrix ensures low levels of nonspecific binding and low levels of host cellderived impurities in the elution pool.
Fulfills industrial demands for security of supply, robust performance, and regulatory support.
rProtein A Sepharose 4 Fast Flow is based on the well established Sepharose Fast Flow platform and is used in many approved monoclonal antibody (MAb) processes. It is composed of recombinant protein A coupled to the crosslinked 4% agarose base matrix. The recombinant protein A is produced in E. coli and has been specially engineered to favour an oriented coupling giving a matrix with enhanced binding capacity. The epoxy based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A, giving a high purification factor in a single step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose 4 Fast Flow suitable for purification of monoclonal antibodies at both laboratory and process scale.