Sephadex G-100 DNA Grade SF, 100 g
For purification of DNA from small molecules by gel filtration in spin columns with an exclusion limit of 25bp ds DNA
Tested to ensure reproducibly high recovery of DNA.
Suitable for researchers who prefer to prepare their own columns for nucleic acid purification
Sephadex G5NA DNA Grade is used in illustra AutoSeq G5NA columns, ProbeQuant G5NA Micro Columns, MicroSpin G5NA Columns, and NICK Column.
Sephadex G100 is one of five different Gtypes ranging from G10 for small molecules to G75 for larger molecules. Spin columns packed with Sephadex G100 DNA Grade allow for highrecovery nucleic acid purification via gel filtration. Gel filtration resins do not exhibit a fixed exclusion limit when used in a spincolumn format. In spincolumn chromatography, the observed exclusion properties that allow the product to pass through the gel while the smaller impurities are retained depend on experimental factors, such as the resin used, sample volume, product size, and the gforces used in the purification process.
Sephadex G50 is a wellestablished gel filtration resin for desalting and buffer exchange of biomolecules >30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length. Sephadex G25 has an exclusion limit of approximately Mr 5000, and when packed into empty MicroSpin columns, the resulting desalting columns can be used in a spin protocol for DNA and oligo purification of any DNA greater than 10 bases in length.
All these Sephadex types are therefore highly suitable for the purification of oligonucleotides or small DNA fragments following synthesis or in labeling applications.