Sephadex G-25 DNA Grade SF, 100 g
For purification of DNA (ge/ 1NA bases in length) from small molecules by gel filtration.
Tested to ensure reproducibly high recovery of DNA.
Used in MicroSpin G25 Columns and NAP Columns.
Enables self preparation of columns for nucleic acid purification.
Sephadex G25 is one of five different Gtypes ranging from G10 for small molecules to G75 for larger molecules. Sephadex G25 has an exclusion limit of approximately Mr 5000, and when packed into empty MicroSpin columns, the resulting desalting columns can be used in a spin protocol for DNA and oligo purification of any DNA greater than 10 bases in length.
Sephadex G50 is a wellestablished size exclusion chromatography resin for desalting and buffer exchange of biomolecules > 30 000 molecular weight, and with a spin protocol can be used for DNA and oligo purification of molecules greater than 20 bases in length.
Alternatively, Sephadex G100 DNA Grade can be used. Sephadex G100 DNA Grade has an exclusion limit of 25bp ds DNA. All these Sephadex types are therefore highly suitable for the purification of oligonucleotides or small DNA fragments following synthesis or in labeling applications.