His GraviTrap, 10 × 1 mL
For fast and simple purification of histidinetagged* proteins.
Convenient, prepacked gravityflow column.
Allows direct purification of both clarified and unclarified cell lysates.
High binding capacity; approximately 4NA mg of pure protein per column.
Compatible with a wide range of reducing agents, detergents, denaturants and other additives.
The ready to use column is prepacked with Ni Sepharose 6 Fast Flow, which has a high protein binding capacity, low nickelion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.
His GraviTrap columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD1NA Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 1NA ml.