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Cytiva, now with the life sciences business from Pall Corporation, is a global biotechnology leader dedicated to helping customers discover and commercialize the next generation of therapeutics. Cytiva brings dedicated technical expertise and a broad portfolio of tools, technologies, and services that enable the development, manufacture, and delivery of transformative medicines to patients.

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Chromatography

Chromatography Consumables

Chromatography Sample Preparation Devices

Chromatograph Columns

Reagents and Buffers

Liquid chromatography LC columns

Chromatography Media

Buffers for Protein Methods

$0~$10(0)
$10~$25(2)
$25~$50(5)
$50~$75(2)
$75~$100(6)
$100~$200(25)
$200~$300(52)
$300~$400(15)
$400~$500(23)
$500~$750(48)
$750~$1000(33)
$1000~$5000(100)
$5000~$10000(14)
$10000~$50000(1)
$50000~$100000(0)
$100000~$3000000(0)

Additives

Without: Calcium, magnesium

Without: Phenol red

Without: Calcium, magnesium, phenol red

Application

For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures

Purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®

Purification is done under physiological conditions and mild elution preserves the activity of the target protein

Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes

Cell isolation

Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins

For screening of selectivity, binding and elution conditions, as well as small scale purifications

Can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design

Highly suitable for preparative purification of histidinetagged recombinant proteins

For purification of DNA from small molecules by gel filtration

GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

For the removal and/or purification of serine proteases e.g. thrombin

Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag

Designed for use with a syringe, peristaltic pump, or chromatography system

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

Partial purification of an IgE-stimulating factor from a human T-cell line
Removal of BSA from an HIV-2 virus lysate
Purification of anti-mouse Fc IgG from sheep anti-serum

They can be used for any DNA greater than 10 bases in length and are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction

Average sample loading capacity

400 mg

50 mg

Application_4

Chromatography system|Manual

Cell isolation

Centrifugation

Chromatography system

Bed Dimensions

16 x 100 mm

16 x 600 mm

10 x 100 mm

26 mm x 400 mm

6.4 x 100 mm

7 x 25 mm

16 x 25 mm

26 x 600 mm

16 x 30 mm

3.2 x 300 mm

0.7 x 2.5 cm

1.6 x 2.5 cm

Bed Height With Two Adaptors

0 to 200 mm

0-280 mm

85 mm-350 mm

58 mm - 106 mm

380 mm-650 mm

546 mm-606 mm

650 mm-950 mm

80 mm-350 mm

0 mm-26 mm

158 mm-206 mm

96 mm-156 mm

680 mm-950 mm

Application_5

Chromatography system|Manual

Bed Support

Filter

Frit

Net

Bed Volume

~ 2.4 mL

1 mL

320

1 ml TALON® Superflow /column

120

212 mL

5 mL

Bed Height With One Adaptor

0 mm-29 mm

225 mm-350 mm

525 mm-650 mm

85 mm-109 mm

140-280 mm

230 mm-350 mm

810 mm-950 mm

129 mm-159 mm

185 mm-209 mm

825 mm-950 mm

579 mm-609 mm

Bed Height

25 mm

30 mm

100 mm

600 mm

300 mm

Avoid Using

Not stable below pH 2 nor to strong oxidising agents

Oxidizing agents, citrate buffers

Chelating agents such as EDTA, EGTA , citrate

Bead type

Mag Sepharose Magnetic Beads

Binding Capacity/Column

up to 15mg histidine-tagged protein

At least 10 mg histidine-tagged protein/ml sedimented medium.

Approx. 40 mg histidine-tagged protein/ml medium

125 mg human IgG/column

Binding Capacity/ml Chromatography Medium

Up to 20 mg histidine-tagged protein

> 25 mg horse liver GST/mL resin

>10 mg/mL

>Approx. 10 mg GST-tagged protein/mL resin

~ 13 mg ovalbumin

At least 10 mg histidine-tagged protein

Approx. 35 mg human lgG/mL at 2.4 min residence time

Approx. 40 mg histidine-tagged protein

At least 40 mg histidine-tagged protein/mL resin

BioProcess resin

yes

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

Certificate of Analysis

Yes

Certifications/Compliance

Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

Chemical Stability

Stable in aqueous buffers commonly used in Protein G chromatography, 6 M guanidine hydrochloride pH 4.7, 20 mM sodium phosphate + 1% SDS, 70% Ethanol, 95% Ethanol, 20 mM Sodium phosphate, 100 mM Glycine- phosphoric acid, 6 M Urea, 20% ethanol + 2% hibitan

Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea

Stable in most aqueous and organic eluent systems.

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to acetone, ketones, chlorinated hydrocarbons, aliphatic esters, phenol, NaOH, >10% HCl, >5% acetic acid, or strong mineral acid|å¯èinå—æ¶²ç›¸å±‚æžä½¿ç”¨çš,æ°´æin

Stable in all commonly used aqueous buffers

Commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature

Conditions where the resin may be operated without significant change in function:
in¢ Trifluoroacetic acid, up to 0.2%
in¢ Isopropanol, up to 5%
in¢ Dithiothreitol (DTT), up to 5 mM
in¢ Acetonitrile, up to 70%
in¢ Formic acid, up to 1

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile

The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol

For Ni²⁺-stripped medium 0.01 M HCl, 0.1 M NaOH. Tested for one week at 40°C 1 M NaOH, 70% HAc. Tested for 12 h 2% SDS. Tested for 1 h 30% 2-propanol. Tested for 30 min

Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 0.1 M acetic acid, 8 M urea, 6 M guanidine HCl, 24 % ethanol

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea

Stable in aqueous buffers commonly used in Protein A Chromatography

Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl

Stable in all commonly used buffer systems

Commonly used aqueous buffers

Commonly used aqueous buffers,1 M sodium hydroxide6, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Stable in commonly used aqueous buffers, 1 M NaOH

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Chromatography technique

Antibody affinity chromatography.

Group specific affinity

Multimodal ion exchange chromatography

Affinity coupling

Antibody affinity chromatography

GST-tagged protein purification

Histidine-tagged protein purification

Hydrophobic Interaction Chromatography

Ion Exchange Chromatography

Column i.d.

5 mm

3.2

26 mm

50 mm

10 mm

16 mm

6.4

Color

Colorless to slightly yellow

White

Column volume

8 mL

1 mL

Density Max.

1.078 g/ml

1.135 g/ml|

1.2 g/ml

Column Capacity

< 1 mg/mL

Dimensions

H x W x D: 660 x 860 x 710

Dimensions_29

75 mm

36 mm

Dimensions_1

36 mm

75 mm

Dimensions_33

36 mm

75 mm

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

Estimated Shelf Life from Manufacture Date

3 years

8 years

5 years

Flow Rate

< 1 ml/min

< 5 ml/min

< 2.6 mL/min

1.2 - 2.3

2 ml/min - 10 ml/min

0.5 ml/min - 2.5 ml/min

1 mL/min|1 mL/min

4 ml/min (1 ml), 5 ml/min (5ml)|4 ml/min (1 ml), 5 ml/min (5ml)

< 15 ml/min

< 15 ml/min|< 15 ml/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

5 ml/min - 20 ml/min

< 4 ml/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

< 20 mL/min |< 20 mL/min

> 20 ml/min|> 20 ml/min

1-4ml/min

1.6 mL/min

< 10 ml/minThe pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.|< 10 ml/min å¡«å……åºŠä¸Šæ–¹çš

0.8-3.9 ml/min

< 20 ml/min

< DarcyÂ´s Law

4 ml/min (1 ml), 5 ml/min (5ml)

5 mL/min|5 mL/min

Recommended: 0.5 to 2.0 mL/min

< 20 ml/min|< 20 ml/min

>60 ml/min|> 60 ml/min

1 mL/min - 4 mL/min

< 4 mL/min |< 4 mL/min

< 5 ml/min|< 5 ml/min

0.8â€“3.9 mL/min

< 1 ml/min|< 1 ml/min

< 2.6 ml/min|< 2.6 ml/min

> 20 ml/min

> 4 ml/min

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

< 31 ml/min

>10 ml/min

>60 ml/min

Flow Velocity

< 300 cm/h, using XK 50/30 column with 15 cm bed height

<1800 cm/h

<1800 cm/h|< 1800 cm/h

15 cm/h|15 cm/h

< 600 cm/h

‰¤ 600 cm/h|‰¤ 600 cm/h

< 130 cm/h

< 600 cm/h|< 600 cm/h

â‰¤ 600 cm/h

< 150 cm/h at 25Â°C, HR 16/10 columns, 5 cm bed height

< 500 cm/h

30-150cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

< 150 cm/h

<15 cm/h

Up to 40 cm/h

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

100 cm/h - 300 cm/h 1

< 130 cm/h|< 130 cm/h

< 300 cm/h

< 50 cm/h

< 500 cm/h|< 500 cm/h

< 60 cm/hour|< 60 cm/å°æ—¶

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

< 450 cm/h

< 450 cm/h|< 450 cm/h

10 cm/h - 50 cm/h|10 cm/h - 50 cm/h

For Use With

For all Biacore SPR systems.

Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100

C 10 columns

The volumes are designed for Biacore 8K+, Biacore 8K but the kit can be used with all Biacore SPR systems

Connect a syringe to the top of a HiTrap columns.

HiTrap and HiPrep columns (connecting column to AKTAdesign systems).

PD-10 Desalting Columns

AKTA lab protein purification system such as AKTA go, AKTA pure and AKTA avant

Filtrate Vessel Material

Polycarbonate (PC)

Filter Media

PES filter

Flow Direction

Flow should follow arrows

Supported bidirectionally. Certain applications may require orientation, i.e., vents. Reverse flow only for low pressure applications.

Endotoxin Activity Max.

< 0.12 EU/ml

0.12 EU/mg

2 EU/ml

Exclusion Limit Peptides

4 x 10Â³

Format_6

Bulk resin

Prepacked columns

Kits

Prepacked column

Format

Liquid

Hold-up Volume of Membrane

<5 Î¼l

<20 Î¼l

<10 Î¼l

Group To be Coupled

-NHâ‚‚

Primary amines

Guidance

For AKTAprime

Housing

Polypropylene (PP)

Includes

1 Ã— Column stand 4 Ã— PD-10 spin adaptor 1 Ã— Buffer tray 30 Ã— Bottom sleeve Instructions are supplied with the columns

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Instructions supplied with the concentractors

2 Ã— 100 ml phosphate 8x stock solution (pH 7.4), and 100 ml highly pure 2 M imidazole (pH 7.4)

Dismantling tool, support screen, net ring (10 Âµm), O-ring, stop plugs, and instructions.

Column valve V9-C
PEEK tubing i.d. 0.75 mm/ o.d. 1/16''
PEEK tubing i.d. 0.5 mm/o.d. 1/16''

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

Connectors for high- and low-pressure chromatography equipment and instructions.

Ion Exchanger Type

Weak anion exchanger

Weak and strong anion

Strong anion exchanger

Weak anion

Multimodal weak cation exchanger

Strong cation exchanger

Weak cation exchanger

Ionic Capacity

0.18-0.24 mmol Cl^-/ml medium

0.07-0.09 mmol H⁺/ml medium

0.10â€“0.14 mmol Cl^- / mL resin

0.09-0.13 mmol H⁺/ml medium

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

0.18-0.25 mmol H⁺/ml medium

0.15-0.20 mmol H⁺/mlÂ

40-85 Âµmol/ml (Cl^-)

0.13-0.17 mmol/ml

0.29-0.35 mmol Cl^-/ml medium

0.11â€“0.16 mmol (Cl^-)/mL resin

3-4 mmol/g

Inlet Connector

Stepped barb for 6 to 10 mm (1/4 to 3/8") tubing

Stepped barb for 10 to 12 mm (3/8 to 1/2") tubing

Inlet id

1.55 mm (0.06 in)

1.0 mm (0.04 in)

2 mm (0.08 in)

0.75 mm (0.029 in)

1 mm (0.04 in)

Ion exchanger

S (cation exchanger)

Q (anion exchanger)

Intrinsic Viscosity

~ 17

Kit Contents

RNAspin Lysis Solution (Red sticker): 125 ml, RNAspin Wash Buffer I (Yellow sticker): 80 ml, RNAspin Wash Buffer II (Grey sticker): 3 Ã— 25 ml Add 100 ml Ethanol to each bottle; RNAspin Desalting Buffer (Blue sticker); 125 ml, RNAspin Desalting Reaction B

Anti-histidine antibody: 1 mg/ml in 0.15 M NaCl, 90 Î¼l
Immobilization buffer: 10 mM sodium acetate pH 4.5, 2.6 ml
Regeneration solution: 10 mM glycine-HCl, pH 1.5, 2 Ã— 120 ml

Ovalbumin (50 mg): 43 000 M_r
Conalbumin (50 mg): 75 000 M_r
Aldolase (50 mg): 158 000 M_r
Ferritin (15 mg): 440 000 M_r
Thyroglobulin (50 mg): 669 000 M_r
Blue Dextran 2000 (50 mg

BIAdesorb solution 1, 500 mL
BIAdesorb solution 2, 500 mL

RNAspin Lysis Solution (Red sticker): 25 ml, RNAspin Wash Buffer I (Yellow sticker): 15 ml, RNAspin Wash Buffer II (Grey sticker): 12.5 ml Add 50 ml Ethanol; RNAspin Desalting Buffer (Blue sticker); 25 ml, RNAspin Desalting Reaction Buffer (Green sticker)

Aprotinin (10 mg): 6500 M_r
Ribonuclease A (50 mg): 13 700 M_r
Carbonic anhydrase (15 mg): 29 000 M_r
Ovalbumin (50 mg): 43 000 M_r
Conalbumin (50 mg): 75 000 M_r
Blue Dextran 2000

Licensing

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

Ligand

Calmodulin

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Native protein A

TALONÂ® Superflow Cobalt-IMAC

Bovine gelatin

Glutathione and 10-carbon linker arm

N-hydroxysuccinimide (NHS) activated

Phenyl

Diethylaminoethyl

Alkali-stabilized Protein A-derived (E. coli)

Cibacron Blue

Human IgG

Quaternary amine

Carboxymethyl

Porcine heparin

Recombinant protein A (E.coli)

Sulphopropyl

Alkaline-stabilized protein A-derived (E.coli).

Octylamine

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Ligand Coupling Method

Ether linkages and hydrophilic spacer arm

Ether linkages

Cyanogen bromide activation

Triazine

Epoxy activation

Dextran surface extenders

Epoxy

Maleimide activation

Reductive Amination

Ligand Density

3 mg GammaBind G, type 3/ml medium

3 mg Protein A/ml medium

Approx. 13 Î¼mol/ml

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

~ 7 Î¼mol Cibacron Blue/ml medium

2 mg Protein G/ml medium

~ 5 mg/mL resin

6 mg rProtein A/ml medium

Ligand_7

Protein G

Butyl, octyl, phenyl

Protein L

Protein A, alkali stablilized

Protein A

Protein A, alkali stabilized

2-mercaptopyridine

Ligand Concentration

~45 Âµmol phenyl/mL resin

~25 Âµmol phenyl/mL resin

4.5-8 mg gelatin/ml drained resin

~4mg heparin/mL resin

Matrix

6% cross-linked agarose

Composite of cross-linked agarose

Highly cross-linked agarose with dextran surface extender

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

cross-linked agarose, 4%, spherical

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Cross-linked agarose, 4%

agarose, 6%, spherical

cross-linked, 6% agarose particles

Highly cross-linked agarose

Composite of cross-linked agarose and dextran

Highly cross-linked 6% agarose

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

2% agarose

Cross-linked dextran

Material

Polypropylene (PP)

Polyetheretherketone (PEEK) Polytetrafluoroethene (PTFE)

Polycarbonate (PC)

Acetal plastic (POM)

Matrix

4% Agarose

4% Cross-Linked Agarose

Highly cross-linked agarose, spherical.

Rigid, cross-linked agarose, spherical

Highly cross-linked 4% agarose

Metal Ion Capacity

approx. 15 Âµmol Ni²⁺/ml medium

54 to 70 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Operating Flow Rate, Recommended

1.3 mL/min

0.50 mL/min

0.5 mL/min

0.75 mL/min

0.04 mL/min

0.075 mL/min

0.80 mL/min

0.30 mL/min

0.45 mL/min

Operating Flow Rate, Max

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol at room temperature;
0.35 mL/min water at low temperature;
0.15 mL/min 20% ethanol at low temperature

1.0 mL/min

2.6 mL/min

1.50 mL/min, water at room temperature;
0.75 mL/min, 20% ethanol at room temperature;
0.75 mL/min water at low temperature;
0.35 mL/min 20% ethanol at low temperature

0.15 mL/min, water at room temperature;
0.07 mL/min, 20% ethanol at room temperature;
0.07 mL/min water at low temperature;
0.03 mL/min 20% ethanol at low temperature

1.2 mL/min, water at room temperature;
0.60 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.60 mL/min water at low temperature;
0.30 mL/min 20% ethanol or 10% glycerol at low temperature

1.80 mL/min, water at room temperature;
0.90 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.90 mL/min water at low temperature;
0.45 mL/min 20% ethanol or 10% glycerol at low temperature

< 4 mL/min

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.35 mL/min water at low temperature;
0.17 mL/min 20% ethanol or 10% glycerol at low temperature

1.60 mL/min, water at room temperature;
0.80 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.80 mL/min water at low temperature;
0.40 mL/min 20% ethanol or 10% glycerol at low temperature

Operating Temperature

4Â°C-35Â°C

4Â°C-60Â°C

Order code

SH30588.02

SH30031.FS

SH30237.01

SH30031.02

SH30268.01

SH30588.01

SH30031.01

Order Information

This product is part of our Custom Designed media program and not yet a standard product. If you are interested in large-scale quantities, please contact your local GE Healthcare representative. This product is produced based on a customer order. Delivery

For research use only.

Not for use with ultrasalt-sensitive automated DNA Sequencers (e.g. ALFexpress II and ABI 377 model.)

Percoll PLUS is for research use only.

Allows safe, reliable storage and transport of self-packed Tricorn columns.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

Columns included in HiTrap HIC Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

Disposable columns for clean-up of proteins/oligosaccharides. The columns are prepacked with Sephadex G-25 resin and for sample volumes up to 2.5 mL by gravity flow and columns spin flow. PD- 10 Buffer reservoir has to be ordered separately For smaller s

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Tricorn high-performance columns have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Column not suitable for use with AKTAprime plus system.

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

HiLoad columns are prepacked XK columns.

Not suitable for use with AKTAprime plus system.

Columns included in HiTrap IEX Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

The use of these products in Biacore systems requires an Amine Coupling Kit with Sensor Chip CM5, CM4, CM3, or C1.

HiLoad columns are prepacked XK columns

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

This product is produced based on a customer order. Delivery time may be longer than for standard products.

Osmolality

< 25 mOsm/kg

< 30 mOsm/kg

288-310 mOsm/kg

Pack size

1000 mL

15 g

1 L

1 Ã— 1 mL

4.7 mL

5 Ã— 5 mL

50 preps

6 x 100 mL

75 mL

1.7 mL

100 mL

125 mL

25 g

100 g

20 columns

30 columns

5 mL

1 Ã— 320 mL

1 Ã— 4.7 mL

10 g

2 x 1 mL

250 columns

500 mL

53 mL

1 kit

1.5 g

10 Ã— 2 mL

5 Ã— 1 mL

6 x 1 L

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 Ã— 100 ÂµL

50 columns

6 mL

7 Ã— 1 mL

10 mL

200 mL

25 mL

4 x 50 mL

4 x 53 mL

500 g

6 x 500 mL

2 x 1 ml 10% slurry

50 mL

1 Ã— 120 mL

1 piece

20 mL

250 preps

300 mL

Partical Size Dry

>20 Âµm

20 Âµm-50 Âµm

40 Âµm-120 Âµm

>100 Âµm

50 Âµm-150 Âµm

18 Âµm-111 Âµm

Particle Size

24 Âµm-44 Âµm

25 Âµm-75 Âµm

30 Âµm-40 Âµm

60 Âµm-165 Âµm

45 Âµm-165 Âµm

45 Âµm-165 Î¼m

45 Î¼m-165 Î¼m

24-44 Âµm

60 Î¼m-200 Î¼m

60 Âµm

60 Î¼m-160 Î¼m

Particle size distribution, wet

100â€“300 Âµm

20â€“80 Âµm

55â€“165 Âµm

87â€“510 Âµm

15â€“88 Âµm

90â€“280 Âµm

Particle size, d_50V

~100 Î¼m

~34 Âµm

~34 Î¼m

~ 9 Âµm

ImpRes = ~ 40 Î¼m, otherwise = ~ 75 Âµm

~75 Âµm

~90 Î¼m

~ 8.6 Âµm

~50 Âµm

~85 Âµm

~90 Âµm

34 Âµm

Outer Diameter

12 or 18 mm

10-21 mm

pH Stability Cleaning-in-Place (CIP)

1 to 14

pH stability, CIP

2 (ligand dependent)â€“13 (ligand dependent)

2â€“9

4â€“9

3â€“13

2â€“10

3â€“14

2â€“10.5

3â€“10

4â€“13

2â€“13

2â€“13.7

3â€“12

2â€“12

1â€“14

2â€“14

2â€“14Co²⁺-stripped medium

pH stability, operational

2â€“9

4â€“13

4â€“9

2â€“12

2â€“13

4â€“12

3 to 12

3â€“11

3â€“13

3â€“10

2â€“10

pH 6 to 10

3â€“12

3â€“9

Osmolality

322-344 mOsm/kg

276-298 mOsm/kg

Outlet Connector

Stepped barb for 6 to 10 mm (1/4 to 3/8") tubing

Stepped barb for 10 to 12 mm (3/8 to 1/2") tubing

Outlet id

0.75 mm (0.029 in)

1.0 mm (0.04 in)

1.55 mm (0.06 in)

pH ligand fully charged

Above 6

< 9

Entire pH range

Below 9

Particle size distribution, dry

20 â€“ 80 Âµm

50 â€“ 150 Âµm

Pore Size

1 Âµm

0.2 Âµm

0.45

Pressure/Flow Specification

24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d

Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 150 cm/h, bed height 10 cm, column 5 cm i.d.

>480 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

>11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height

60-120 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.

base matrix 70-140 cm/h, pressure drop cm Hâ‚‚O/bed height=15 cm, bed height 10 cm, 5 cm i.d.

>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height

min 90 cm/h, bed height 10 cm, column 5 cm i.d.

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

pH Range

8.9â€“9.9

8.5â€“9.5

5.5â€“7.5

Operating Pressure Max

4.1 bar (60 psi)

Pressure/flow characteristics

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)

150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Power Consumption

8 VA

Particle Size in Methanol Max

163

Physical State

solid

liquidSuspension

liquid

Porosity

10 or 80 Âµm

7 Î¼m

20 Î¼m

10 Î¼m

Pressure Limit

20 bar (2 MPa, 290 psi)

2 MPa

7.0 to 7.4

7.2 - 7.5

Physical Form

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Properties

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Particle Size in Methanol Min

pH Stability Working Range

3â€“12

Recommended Flow Rate

1 ml/min

5 ml/min

Relative Humidity

20%-95% (non condensing)

Recommended maximum operational flow velocity

300 cm/h

Sample Volume

2.5 ml

400 to 1000 Î¼l

< 0.5 mL

< 5 mL

< 150 Âµl

100-180 Î¼l

<= 1.5 ml

<= 13 ml

4 to 50 ÂµL

0.1 to 0.5 ml (Gravity), 0.2 to 0.5 ml (Spin)

25 to 500 ÂµL

600 Âµl

0.5 to 1 ml (Gravity), 0.75 to 1.0 ml (Spin)

< 100 Âµl

<= 15 ml

<= 5 ml

100 to 300 Î¼l

Up to 8.5 mL

Scale

Lab|Process

Lab|Process development

Lab

Lab|Process development|Process

Sanitization Conditions

2% Hibitane/20% Ethanol or with 70% Ethanol

Resin Type

Capto

Sepharose Fast Flow

Sepharose High Performance

MabSelect

Sepharose CL-4B

Superdex 200 Increase

Shelf Life

24 Months

12 months

Sealing

Heat-fused

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Storage

2 to 8Â°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol

2Â° to 8Â° C

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, 20% Ethanol, 0.05 M Sodium Acetate

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

2 to 8Â°C, 100% Isopropanol

4 to 30Â°C, 20% Ethanol or 70% Acetonitrile

4 to 30Â°C, in 20% Ethanol

2 to 8Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0

2Â°Câ€“8Â°C, 20% ethanol

15Â° to 30Â° C

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

2ËšC to 8ËšC, 20% ethanol

4 to 30Â°C, 20% Ethanol (Q, DEAE, ANX,CM), 20% Ethanol, 0.2 M Sodium Acetate (SP)

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

4 to 30ËšC

4Â°C to 8Â°C

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol

4 to 30Â°C, 0.05 M Sodium Acetate containing 20% Ethanol

4â€“30Â°C, 20% Ethanol

+4Â°C to +8Â°C

2 to 8Â°C

2 to 8ÂºC, 20% Ethanol, 50 mM Kâ‚‚HPOâ‚„, 1mM MgClâ‚‚ pH 7.2 in 20% Ethanol

20 to 25Â°C, Double distilled water containing 0.05% Kathon CG/ICP Biocide as preservative

20 to 25Â°C, TE buffer (10 mM Tris/HCl, 1mM EDTA) containing 0.05% Kathon CG/ICP Biocide as preservative.

4 to 30Â°C

4 to 30Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

4 to 30Â°C, 0,05% Kathon CG/ICP Biocide

4 to 30Â°C, 0.15% Kathon CG

4 to 30Â°C, 20% Ethanol (Q, DEAE), 20% Ethanol, 0.2 M Sodium Acetate (S)

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Stability

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stable

Storage Conditions

Store at room temperature.

20% ethanol at 4Â°C to 30Â°C

4 - 30Â°C

4 - 8Â°C, 20% Ethanol, 10% medium slurry

4ËšC to 30ËšC, 20% ethanol

At room temperature. Precipitation can occur at low temperature.

4â€“30Â°C

Store at 2Â°C to 8Â°C

Support System

Polypropylene (PP)

Sterilization

0.1 Âµm sterile filtered

May be autoclaved at 121Â°C for 20 minutes. Multiple autoclave cycles are possible. However, the responsibility for reuse is with the operator. The device should be protected from cross contamination. An integrity test should be performed after autoclavin

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Stokes Radius

~ 10

Swelling factor

~1.7

System

Sepax C-Pro|Sefia|Sepax 2

Theoretical Plates

> 48 000 m^-1

> 38 000 m^-1

> 13000 m-1

> 43 000 m^-1

> 42 000 m^-1

5000 m-1

Total binding capacity

~50 mg hIgG/mL resin

Total Time of Preparation

< 30 min/6 preps

Typical Pressure Drop Over the Packed Bed

~ 3 MPa

~ 2 MPa

Volume

2-6 ml

400 Âµl - 2 ml

100-500 Âµl

5-20 ml

Viscosity Max

Volume_28

2-6 ml

100-500 Âµl

5-20 ml

400 Âµl - 2 ml

Wetted Part

yes

Well Volume

8.5

Type of ion exchanger

Strong anion exchanger (quaternary ammonium)

Strong cation exchanger (methyl sulfonate)

Water Flow Rate at 10 bar (145 psi)

Wetted Materials

Polyetheretherketone (PEEK)/Polytetrafluoroethylene (PTFE)

Polyetheretherketone (PEEK) Polytetrafluoroethene (PTFE)

Vent

On inlet

Wetted materials1

Polyetheretherketone (PEEK)/Polytetrafluoroethylene (PTFE)

Wetting Characteristics

Hydrophilic

Exclusion Limit

4 x 10³ (for Peptides) 5 x 10³ (small organics)

For use with_31

AKTA go protein purification system

Recommended Cleaning-in-Place Reagent

2 M NaCl solution

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