Government Scientific Source

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$0~$10(0)

$10~$25(0)

$25~$50(7)

$50~$75(24)

$75~$100(34)

$100~$200(91)

$200~$300(78)

$300~$400(51)

$400~$500(35)

$500~$750(60)

$750~$1000(39)

$1000~$5000(66)

$5000~$10000(7)

$10000~$50000(11)

$50000~$100000(0)

$100000~$3000000(0)

Absorbance

0.04 max. at 210nm

Anhydrousor Hydrate

Anhydrous

Additional Information

Vapor Pressure: 97mbar at 20°C

Antibiotic ResistanceBacterial

Ampicillin (AmpR)

Application_19

High-throughput preparation of circular DNA templates for cycle sequencing, cloning, and transformation.

Assay Percent Range

>=99.9 %

BacterialorYeastStrain

Not Included

Average Particle Size

400 nm diameter

700 nm diameter

Boiling Point

81°C

Brand

Axygen®

Life Sciences

CAS Registry Number 1

75-05-8

9007-49-2

CAS_NO

57-55-6

Certificate of Analysis

Yes

Chem AlertStorageSymbol

Red

ChEBI

CHEBI:38472

Chemical Name or Material

Glycogen

Acetonitrile

Country of Origin

Chemical Substance ID

24848147

Description

Bacterial/Yeast Strain: DH10B

Collect, stabilize, process, transport, and archive blood samples with Whatman Non-Indicating FTA Classic Cards.

MagJET Separation Rack, 12 x 1.5mL tubes

Color

Green

Colorless

Content AndStorage

• Lysis Solution, 40 mL
• Precipitation Solution (10X), 8 mL
• 1.2 M sodium chloride, 10 mL

Store at room temperature.

Cleanup Symbol

Solvent

Decomposition Information

>500°C

DOT Information

DOT Class 3, : Flammable Liquid

EC Number

200-338-0

Cloning Method

Restriction Enzyme ⁄ MCS, Blunt PCR

For Use With Application

Isolation of DNA from white Blood cells, tissue culture cells, animal tissue, plant tissue, yeast and gram positive and gram negative bacteria

Efficient removal of unincorporated dNTPs, salts and Enzymes

Isolate bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples

PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription

Rapid and efficient purification of DNA and Oligonucleotides

Forensics.Transgenic identification.Transfusion medicine/HLA typing.Plasmid screening.Food and agriculture testing.Drug discovery.Genomics.STR analysis.Animal identification.Whole genome amplificationMolecular biology.

Break hard/brittle samples

Automated genomic DNA extraction from whole blood samples collected in EDTA, ACD or heparin tubes

Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Sequencing, PCR

Purifies linear and circular DNA from 200 to 50,000bp

Isolate genomic DNA from whole blood, cultured cells, yeast, mouse tissue or numerous other cell sources

Sample Preparation; Sample Lysis; Nucleic Acid Isolation & Purification; Molecular Biology; Gene Expression; Microbiological Analysis; Gene Function; Gene Regulation

Clean sample cuts from FTA/FTA Elute cards

Ideal for applications requiring multiple paramagnetic isolations of biomolecules

Cloning (Blunt PCR, Restriction Enzyme G?° MCS)

Concentration

4mg/mL

Density

0.781g/cm-?

For Use With Equipment

Magnetic Stand

Wizard genomic DNA purification kit

Wizard SV Gel and PCR cleanup system

MagneSphere and PolyATtract system components

Harris Micro Punch

Direct STR amplification

Maxwell 16 and 16 MDx instruments

FastPrep-24; FastPrep 120; FastPrep-96; SuperFastPrep-1

Form

liquid

Format

Powder

Estimated Shelf Life from Manufacture Date

24 months

Export control classification number

EAR99

Formula

Service & Support

CH₃CH(OH)CH₂OH

Fluorescence Background

Pass Test

Format

Four sample areas

Tube(s)

Spin or vacuum

96 and 384-well plate

Formula Weight

41.05

HAZ

Grade

DNA Synthesis

Harmonized Code US

3822005090

Hazardous

Yes

Highthroughput Compatibility

Compatible (Vacuum manifold)

Not High-throughput Compatible (Manual)

Health Hazard 2

DANGER!

Health Hazard 3

Emergency Overview
Flammable liquid and vapor. Irritating to eyes. May cause skin and respiratory tract irritation. May cause central nervous system effects. Liver and kidney injuries may occur. Use personal protective equipment. Use only unde

items per unit of measure

1 each

1 kit

IUPAC Name

acetonitrile

Kit Contents

Sample Buffer 1 x 0.9 ml; Reaction Buffer 1 x 0.9 ml; Enzyme Mix 1 x 100 Âµl; Control DNA (Lambda), 10 ng/Âµl 1 x 20 Âµl

Sample Buffer (white cap) 1 Ã— 0.5 ml; Reaction Buffer (Blue cap) 1 Ã— 0.5 ml; Enzyme mix (yellow cap) 1 Ã— 20 Âµl; Positive control DNA (pUC19, 2 ng/Âµl) 1 Ã— 50 Âµl

Sample Buffer (white cap) 5 Ã— 0.5 ml; Reaction Buffer (Blue cap) 5 Ã— 0.5 ml; Enzyme mix (yellow cap) 5 Ã— 20 Âµl; Positive control DNA (pUC19, 2 ng/Âµl) 1 Ã— 50 Âµl

TempliPhi premix 1 x 20 ml; Denature buffer 10 mM Tris-HCl, pH 8.2, 0.5 mM EDTA, 1 x 20 ml; Positive control pUC19 DNA 1 x 10 Î¼l, 2 Î¼g/ml

Sample Buffer 1 x 225 Âµl; Reaction Buffer 1 x 225 Âµl; Enzyme Mix 1 x 25 Âµl; Control DNA (Lambda), 10 ng/Âµl 1 x 20 Âµl

Single Cell GenomiPhi Reaction buffer 1 x 275 Âµl, Single Cell GenomiPhi Enzyme mix 1 x 25 Âµl, Single Cell GenomiPhi Amplification mix 1 x 25 Âµl, Single Cell GenomiPhi DTT Solution 1 x 100 Âµl, Single Cell GenomiPhi Lysis buffer 1 x 100 Âµl and Single C

Sample Buffer 10 x 2.25 ml; Reaction Buffer 10 x 2.25 ml; Enzyme Mix 10 x 250 Î¼l; Control DNA (Lambda) 10 ng/Î¼l 10 x 20 Î¼l

L CMSGradientSuitability

Pass Test

PAC code

7132

7280

PAC Code Description

MFT GX & XT Consumables

MFT GX & XT Reagent

Percent Purity

>=99.9%

Includes

100mL Wizard™ DNA cleanup resin, 100 Wizard™ minicolumns, 100 3cc Syringe barrels

Product Gross Weight Desc

Product Line

GeneJET

CloneJET

MagJET

Product Type

Accessory

FTA Classic Card, 4 sample areas per card

Genomic DNA

NAP-5

NAP-10

RNase A Solution, 4mg/mL

Wizard DNA Cleanup System

LEV Blood DNA Kit

Kit

NAP-25

System

2-Position

Genomic DNA Purification Kit

Lysing Matrix A (garnet)

Solution

Melting Point

-46°C

Molecular Weight

0.0

76.09

Qty/Pk

1 / Pk

Quantity

1mL

100 Pack

20 Reactions

20mL

48 Preps

5 mL

250 Preps

10mg

100 preps

12 x 75mm

50 Preps

50 Pack

Recommended Storage

Keep cold

Sample weight1

1 pgâ€“10 ng of purified plasmid or M13 DNA; bacterial or M13 liquid cultures, colonies, plaques or stocks

Size

1,000u

1.5ml

250 preps

250microg

50ml

100 reactions

200 preps

50 preps

500 isolations x 300microl

100 preps

25ml

32.5microg

500u

5microg

1 x 96 preps

12 x 75mm

250microl

30 reactions

500microl

50microg

100microg

25/pack

50microl

25 preps

100 isolations x 300microl

100u

125ml

150 reactions

48 preps

5 entry and 20 transfer reactions

500 reactions

90microg

1 each

1ml

2 x 96 preps

4 x 96 preps

SMILES

CC#N

Species

Human

Stability

This product has been designed to deliver high quality results for up to 12 months from the manufacturing date. Please refer to the expiration date on the product label.

Stability_25

The TempliPhi premix is stable at -70Â°C for 6 months from the date of manufacture. The denature buffer and positive control DNA are stable at -70Â°C or -15Â°C to -30Â°C for 12 months from the date of manufacture

Includes

100mL Cell lysis solution, 50mL Nuclei lysis solution, 25mL Protein precipitation solution, 50mL DNA rehydration solution, 250µL RNase solution

50 x 2mL Tubes Lysing Matrix E, 60mL Sodium Phosphate Buffer, 8mL MT Buffer, 25mL PPS Solution, 66mL Binding Matrix, 50 spin modules, 50 catch tubes, 12mL concentrated SEWS-M, 20mL DES, 200µL BBS Gel loading dye, user manual

Complete step-by-step protocol identifying the fixed volumes needed for equilibration, washing and sample elution

[other]

48 Maxwell 16 MCD LEV cartridges, 50 LEV purification plungers, 50 Elution tubes, 20mL Lysis Buffer, two 1mL Proteinase K solution, 20mL Elution Buffer

No of Reactions

50 preps

48 preps

250 Preps

Order Information

Custom options of the product are available. Please contact your local sales representative or Technical Support to request. Larger pack sizes available on request.

Custom options of the product are available. Please contact your local sales representative or Technical Support to request., Larger pack sizes available on request; please inquire.

Pack size

25 reactions

2000 reactions

500 reactions

1000 reactions

100 reactions

Physical Form

Powder

Liquid

Product Size Avg

> 10 kb

>10kb

Quantity

40 reactions

200g

Each

Sample Size

125µL per sample area

300µL

MDL Number

MFCD00166026

MFCD00064272

InChIKey

WEVYAHXRMPXWCK-UHFFFAOYSA-N

Storage Requirements

15 to 30°C

Dry Ice (-80°C)

+15C to +30C

15 to 30°C

Room Temperature

Input Amount

1-1000 cells or 1fg-10ng of input DNA

Item Class Description

Consumables

Reagents

Storage Conditions

The product can be stored for 2 months or less at -20Â°C. For storage longer than 2 months, store at -70Â°C Thaw components on ice and maintain at 0â€“4Â°C during handling. DO NOT warm above 4Â°C prior to amplification

Store the kit at -70Â°C. The enzyme mix must be stored at -70Â°C; all other components may be stored at -20Â°C. Thaw components on ice and maintain at 0Â°C to 4Â°C during handling.

Store Denature buffer and control DNA at -70Â°C or -15Â°C to -30Â°C. Store TempliPhi premix at -70Â°C.Do not store TempliPhi premix at -15Â°C to -30Â°C. Thaw the premix on ice and maintain at 0â€“4Â°C during handling. Do not allow premix to warm above 4Â°

Store the kit at -70Â°C. The enzyme mix must be stored at -70Â°C; all other components may be stored at -20Â°C. Thaw components on ice and maintain at 0Â°C to 4Â°C during handling.

Store the kit at -70ÂºC. The enzyme mix must be stored at -70ÂºC; all other components may be stored at -20Â°C.

Reaction Time

4 h

4-18 h depending on starting material

1.5-2 h

2 to 4 hours

One Unit Contains

1 each

1 kit

Packaging

Glass Bottle

Prep Scale

< 100 µg (Small-Scale) Plasmid DNA

Product Gross weight

0.01

0.2

Product Family

Axygen® AxyPrep MAG PCR Clean-up Kit

No of Samples

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

UNSPSC Code

41106001

41105331

PurificationTarget

Genomic DNA

Plasmid DNA

Qty/CS

1 / Cs

Yield

5 to 15µg/300µL

Radioactive

FALSE

Reaction time1

16-18 h

SampleType

Bacteria

Liquid Samples (e.g. Serum), Plant Samples, Cells, Tissue, Yeast, Bacteria, Blood

Bacteria, Yeast, Tissue, Cells, Buccal Samples

Residueafter Evaporation

1.0ppm max.

Molecular Weight

41.053

UN Number

1648

Molecular Formula

C2H3N

Volume Metric Sample

125 mL

Pub Chem CID

6342

Storage Temperature

Room Temperature

Vector

pJET1.2/blunt

Refractive Index

1.3405 to 1.3425 (at 25°C)

Refrigerated/Frozen

Frozen

Refrigerated

Sample Weight

1 pgâ€“10 ng of purified plasmid or M13 DNA; bacterial or M13 liquid cultures, colonies, plaques or stocks

10 ng of genomic DNA or non-purified cell lysates

Titratable Base

0.0006mEq/g max.

Viscosity

0.36 cP at 20-°C

Vapor Pressure

97mbar at 20-°C

Synonym

1/4 in. Zirconium Oxide Banded Satellite for Grinding

methane, cyano

Water

10ppm max.

Titratable Acid

0.008mEq/g max.

UoM

UoM Description

each

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