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Your source for innovative, indispensable lab equipment, lab supplies, and services. View their wide selection of products for scientific research and education.

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In Stock

Covid-19 Items

Made in USA

Nucleic Acids and Components

Modifying Enzymes

Restriction Enzyme Buffers

DNA Vectors

Restriction Enzymes

dNTPs

Restriction Enzymes Aa - Bz

Enter your price range below

Min

Max

$0~$10(0)
$10~$25(0)
$25~$50(2)
$50~$75(7)
$75~$100(21)
$100~$200(25)
$200~$300(8)
$300~$400(11)
$400~$500(1)
$500~$750(6)
$750~$1000(3)
$1000~$5000(7)
$5000~$10000(0)
$10000~$50000(0)
$50000~$100000(0)
$100000~$3000000(0)

$0~$10(0)

$10~$25(0)

$25~$50(2)

$50~$75(7)

$75~$100(21)

$100~$200(25)

$200~$300(8)

$300~$400(11)

$400~$500(1)

$500~$750(6)

$750~$1000(3)

$1000~$5000(7)

$5000~$10000(0)

$10000~$50000(0)

$50000~$100000(0)

$100000~$3000000(0)

Volume

200µL

Accession Number

NP_005347

Color

Brown

White

Colorless

Concentration

100 mM

10 mM

50 X

>=20?/mg

>160U/mg

10 X

Content And Storage

Contains:
• 10 x 1.25 mL Phire Plant Direct PCR Master Mix (2X)
• 2 x 5 mL Dilution Buffer
• 40 µL Control Primer Mix (25 µm each)
• 10 x 1.25 mL Water, nuclease- free
• 50 µg O'GeneRuler Express DNA Ladder (suffic

Includes:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO
• 50 mM MgCl2 solution

Both Phusion Green HF and GC Buffers provide 1.5

Content AndStorage

Contains:
• 4 x 1.25 mL DreamTaq Green PCR Master Mix (2X), which includes DreamTaq DNA Polymerase, 2X DreamTaq Green buffer, dNTPs, and 4 mM MgCl2
• 4 x 1.25 mL Nuclease-free water

Store at -20-°C.

Includes:
• Phusion U Hot Start DNA Polymerase, 2 U/µL
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.

S

Contains:
• 2 x 1.25 mL Maxima SYBR Green/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with ROX passive reference dye; sufficient for 200 x 25 µL reactions.

G?° 4 x 1.25 mL 2X Phire Hot Start II PCR Master Mix
G?° 1 x 0.5 mL DMSO
G?° 4 x 1.25 mL Water, nuclease free

Store at -20°C.

The RevertAid RT Kit contains:
• RevertAid Reverse Transcriptase
• RiboLock RNase

The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, 10 ++M Forward GAPDH Primer, 10 ++M Reverse GAPDH

• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl2 solution
• 10 mM dNTP Mix

Store at -5 to -30°C.

Contains:
• 2 x 1.25 mL Phire Plant Direct PCR Master Mix (2X)
• 5 mL Dilution Buffer
• 40 µL Control Primer Mix (25 µm each)
• 2 x 1.25 mL Water, nuclease- free
• 50 µg O'GeneRuler Express DNA Ladder (sufficient f

Includes:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl2 solution
Both Phusion HF and GC Buffers provide 1.5 mM MgCl2 in the final

Contains:
• 1 mL Phusion Blood Direct PCR Master Mix (2X)
• 40 µL Universal Control Primer Mix
• 0.5 mL 50 mM EDTA
• 1.5 mL 50 mM MgCl2
• 0.5 mL DMSO
• 1.25 mL Water, nuclease-free
• 50 µg O'GeneRul

Contains:
• 4 x 1.25 mL DreamTaq Hot Start Green PCR Master Mix (2X)
• 4 x 1.25 mL Nuclease-free water

Store at -5 to -30°C.

Includes:

• 2X Phusion Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration)
• Water, nuclease-free
• DMSO

Store at -20°C.

CompatibleBuffer

10x Buffer Tango

10x FastDigest Buffer/FastDigest Green Buffer

Unique Buffer (10x Buffer AarI)

10x Buffer G

Unique Buffer (10x Buffer SacI)

Components

20µg Mass spec grade lyophilized Trypsin/Lys-C Mix, 500µL Resuspension Buffer

DetectionMethod

SYBR

Enzyme

BshTI

Eco31I

EcoR I

Kpn2I

MreI (Sse232I)

Nde I

XmaJI

BpiI (BbsI)

Pvu I

Pvu II

AatII

PacI

BpiI

Hind III

MssI

Bst1107I

MunI

Bgl II

Esp3I (BsmBI)

Mph1103I

Pst I

Sca I

Psp1406I

Xba I

Xho I

Kpn I

KspAI

LguI (SapI)

Nhe I

Not I

Pfl23II

SacI

AarI

BcuI (SpeI)

BglI

Dpn I

AdeI

Eco147I

Eco81I

PagI

FidelityvsTaq

52X

25 X

52 X

2 X

For Use With Application

For PCR application

For tissue dissociation combined with other Enzymes such as Elastase, Trypsin/Papain

Used to lyse E. coli for preparation of plasmid DNA from transformed cell cultures

For the routine subcloning of PCR products

In-solution Protein digestion, in-solution digestion of proteolytically resistant Proteins

PCR product analysis

Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)

Isolation of DNA and RNA from cell and tissue preparations, inactivating many Enzymes, including Endogenous Nucleases

Used to breaks phosphodiester bonds, catalyze the hydrolysis of various Nucleotide polyphosphates

Expression System

Baculovirus

Format

Solid

Crystalline Powder

Lyophilized powder

GeneID Entrez

3932

GCRichPCRPerformance

Medium

High

Low

Gel Compatibility

Acrylamide Gels, Agarose Gels

For Use With Equipment

7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus System, StepOne System

Form

liquid

GCRich PCRPerformance

High

Medium

Format

0.2mL Tube with hinged cap

Liquid

Fidelityvs Taq

1 X

Labelor Dye

Unconjugated

Methylation Sensitivity

Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive

Not dam methylation-sensitive, Not dcm methylation-sensitive, CpG methylation-sensitive

Not CpG methylation-sensitive, dam methylation-sensitive, Not dcm methylation-sensitive

Not CpG methylation-sensitive, dcm methylation-sensitive, Not dcm methylation-sensitive

MethylationSensitivity

Not dam methylation-sensitive, dcm methylation-sensitive, CpG methylation-sensitive

Not dcm methylation-sensitive, Not CpG methylation-sensitive

Not CpG methylation-sensitive, dam methylation-sensitive, Not dcm methylation-sensitive

Not CpG methylation-sensitive, dcm methylation-sensitive, Not dcm methylation-sensitive

Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive

Not dam methylation-sensitive, Not dcm methylation-sensitive, CpG methylation-sensitive

Gene Alias

LCK

Hot Start

Built-In Hot Start

Overhang

3'-A

Blunt

Product Type

Trypsin/Lys-C Mix

Collagenase Type 4

Lysozyme

Proteinase K

Phosphodiesterase I

Includes LabelorDye

Yes

Includes

2X Phusion Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration); Water, nuclease-free; DMSO

2X Phire Hot Start II PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration); Water, nuclease free; DMSO

2 x 1250µL GoTaq Green MasterMix, 2X, 2 x 1250µL Nuclease free Water, blue dye and a yellow dye, GoTaq DNA Polymerase, dNTPs, MgCl2 and reaction Buffers in a single tube

Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL); 5X Phusion Green HF buffer (provides 1.5 mM MgCl2 in the final 1X concentration); 5X Phusion Green GC buffer (provides 1.5 mM MgCl2 in the final 1X concentration); DMSO; 50mM MgCl2 solution

1.2µg pGEM-T easy vector (50ng/µL), 12µL control insert DNA (4ng/µL), 200µL rapid ligation Buffer (2X), 100U T4 DNA Ligase

Room temperature stable beads containing ∼ 2.5 units of recombinant PuReTaq DNA Polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction Buffer, When a bead is reconstituted to a 25µL final volume, the concentration of each dNTP

Inhibitors

Cysteine, EDTA or o-phenanthroline, alpha2-macroglobulin - a large plasma glycoprotein, alpha2-macroglobulin, alpha1-antitrypsin

Gluctathione, Cysteine and Ascorbic Acid

Kinase Family

SRC Kinase Family

Quantity

100 Units

4 x 0.25mL (4 x 25 -µmol of 100mM solution)

500U (2U/µL)

1mL (Total: 40-µmol)

100mg

200 x 50µL rxns

500U

1 Kit

20 Reactions

50 reactions

100U

4 x 1mL (4 x 100 -µmol of 100mM solution)

1250 x 50++L rxns

20µg

100 rxn

1000U

300µL

100 Reactions

100µL

200 rxns

100U (2U/-µL)

100 x 50µL rxns

Quantity

400µL (400 reactions)

250 x 50Î¼L rxns

2,000U

300µL (300 reactions)

3kg

500mg

100 x 20Î¼L rxns

200U

5 x 1,200U

5 x 1mL

50µL

125U

800µL

25U

800μL (800 reactions)

200µL

Mass

10 µg

Shipping Condition

Approved for shipment on Wet or Dry Ice

Dry ice

Molecular Weight

115000

60.6 kDa

Source

Clostridium histolyticum

Tritirachium album

Egg White

Crotalus adamanteus venom

Storage Requirements

<0°C

Ambient temperature in pouch with desiccant

4°C

-30 to -10°C

-20°C

−30 to −10°C

Label Type

Unlabeled

OptimalReaction Temperature

37° C

PCRMethod

qPCR

Product Line

RevertAid

Phusion

Phire

DreamTaq

FastDigest

Maxima

TargetGene

lymphocyte-specific protein tyrosine kinase

Protein Family

Kinases & Inhibitors

No ofReactions

100

200 reactions

Optimal ReactionTemperature

42° C

p H

9.8 to 10.4.5

ReactionFormat

SuperMix or Master Mix

Standalone

Separate Components

ReactionSpeed

Standard

Fast

Restriction Site

BstZI, NotI and EcoRI

LabelorDye

Unconjugated

Primer

Random Primers

No of Reactions

Reaction Speed

Fast

Polymerase

Phusion Hot Start II DNA Polymerase

DreamTaq DNA Polymerase

Phusion Blood II DNA Polymerase

Phire Hot Start II DNA polymerase

Phusion U Hot Start DNA Polymerase

Phusion Hot Start II High-Fidelity DNA Polymerase

Sample Type

RNA

DNA, dsDNA

Optimal Reaction Temperature

37° C

Polymerase

Taq DNA Polymerase

Includes Labelor Dye

Yes

Protein Subtype

Cytoplasmic Tyrosine Kinases

Protein Tag

His Tag

Purityor QualityGrade

Molecular Biology Grade

Kinase Group

Tyrosine Kinases

Research Category

Other Research

Signal Transduction, Immunology

OptimalReactionTemperature

37° C

Packaging

Poly Bottle

SensitivetoHeatInactivation

Yes

Reverse Transcriptase

RevertAid

ShippingCondition

Dry Ice

Reaction Format

SuperMix or Master Mix

Product Type Specs

KOD Hot Start Master Mix

Protein Form

Full Length, Recombinant, Cytoplasmic

Storage Buffer

50mM Acetic Acid (pH <3) Resuspension Buffer

SufficientFor

100 reactions

20 reactions

TargetEntry

LCK

Promoter

Sp6 and T7

Tag Position

C-Terminal

Vector

pGEM-T easy vector I

Species

Human

System Compatibility

LanthaScreen® Antibody, LanthaScreen® Substrate, Z'-LYTE™, Omnia® Bulk Substrate

Type IISRe

TypeIISRe

Yes

Validated Application

Kinase Assay

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