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Cytiva, now with the life sciences business from Pall Corporation, is a global biotechnology leader dedicated to helping customers discover and commercialize the next generation of therapeutics. Cytiva brings dedicated technical expertise and a broad portfolio of tools, technologies, and services that enable the development, manufacture, and delivery of transformative medicines to patients.

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In Stock

Covid-19 Items

Made in USA

Life Science

Microbiology

Microbiology Supplies

Microbiological Analysis Filters

Molecular Biology Reagents and Kits

Susceptibility Testing Discs

DNA Reagents

RNA Reagents

DNA Extraction and Purification

DNA Sequencing

RNA Controls

Enter your price range below

Min

Max

$0~$10(0)
$10~$25(1)
$25~$50(4)
$50~$75(1)
$75~$100(1)
$100~$200(7)
$200~$300(5)
$300~$400(9)
$400~$500(2)
$500~$750(6)
$750~$1000(1)
$1000~$5000(6)
$5000~$10000(2)
$10000~$50000(1)
$50000~$100000(0)
$100000~$3000000(0)

$0~$10(0)

$10~$25(1)

$25~$50(4)

$50~$75(1)

$75~$100(1)

$100~$200(7)

$200~$300(5)

$300~$400(9)

$400~$500(2)

$500~$750(6)

$750~$1000(1)

$1000~$5000(6)

$5000~$10000(2)

$10000~$50000(1)

$50000~$100000(0)

$100000~$3000000(0)

Additives

With: Porcine trypsin, HBSS, EDTA Without: Calcium magnesium

Application

Purification of proteins and peptides in microliter scale

Suitable for use in ECL, western blotting applications. ELISA Immunocytochemistry

They can be used for any DNA greater than 10 bases in length and are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction

For the rapid purification of DNA for a wide range of applications, including desalting, buffer exchange, removal of dye terminators from cycle sequencing reactions and removal of labeled nucleotides from DNA labeling reactions

Application_4

Other

Average Particle Size

400 nm diameter

700 nm diameter

Brand

Life Sciences

Certificate of Analysis

Yes

Chemical Stability

The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules

Color

White

Column Capacity

< 1 mg/mL

Color

Colorless

Diameter

47 mm

50 mm

Dimensions_1

132 mm (diameter)

Dimensions_29

132 mm (diameter)

Dimensions_33

132 mm (diameter)

Estimated Shelf Life from Manufacture Date

12 months

24 months

For Use With

Biacore 8K+, Biacore 8K, Biacore S200, Biacore T200 and Biacore 4000 SPR systems

Biacore 8K+, Biacore 8K, Biacore T200, Biacore S200, and Biacore 4000 SPR systems

The kit is designed for Biacore 8K+ and Biacore 8K SPR systems.

AKTA go and AKTA pure 25 protein purification systems

Fraction collector F9-T

Biacore SPR systems (except for Biacore 4000). The volume supplied in Biotin CAPture Reagent limits use with Biacore 4000, and methodology support for using the reagent with this system is not available.

Fraction collector F9-R

Field245

Plasmid confers resistance to 100 Î¼g/ml ampicillin

Flow Rate

0.01 to 0.50 mL/min

Field of Application

Sediment analysis (Water microbiology and analysis)

Format

Liquid

Format

Circle

Format_6

Circle

Buffer

Application_19

High-throughput preparation of circular DNA templates for cycle sequencing, cloning, and transformation.

Grid Color

Black

Hydrophilic

Yes

Grid Dimension

3.1

Hydrophobic

For Membrane Butler

Interleaving Paper

Yes

Indicator

Pottassium Iodide

Phenolpthalein

Input Amount

1-1000 cells or 1fg-10ng of input DNA

Kit Contents

TempliPhi premix 1 x 20 ml; Denature buffer 10 mM Tris-HCl, pH 8.2, 0.5 mM EDTA, 1 x 20 ml; Positive control pUC19 DNA 1 x 10 Î¼l, 2 Î¼g/ml

Sample Buffer 1 x 225 Âµl; Reaction Buffer 1 x 225 Âµl; Enzyme Mix 1 x 25 Âµl; Control DNA (Lambda), 10 ng/Âµl 1 x 20 Âµl

BIAtest solution, with HBS-EP, 65 mL
BIAnormalizing solution (70%), 90 mL
BIAdesorb solution 1, 2 Ã— 500 mL
BIAdesorb solution 2, 2 Ã— 500 mL
Series S Maintenance Chip
Note: Due to the Biocidal Products Regulation, the BIAdisinfectant solut

Sample Buffer 10 x 2.25 ml; Reaction Buffer 10 x 2.25 ml; Enzyme Mix 10 x 250 Î¼l; Control DNA (Lambda) 10 ng/Î¼l 10 x 20 Î¼l

Ready-To-Go GenomiPhi HY cakes, 3 Ã— 8-well strips; 2x Denaturation buffer, 1 Ã— 2.6 ml; Control DNA (Lambda), 500 ng; 12 Strips of 8 domed caps

Sample Buffer (white cap) 5 Ã— 0.5 ml; Reaction Buffer (Blue cap) 5 Ã— 0.5 ml; Enzyme mix (yellow cap) 5 Ã— 20 Âµl; Positive control DNA (pUC19, 2 ng/Âµl) 1 Ã— 50 Âµl

Biotin CAPture Reagent, 50 Î¼g/mL in HBS-EP buffer (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA, 0.005% Surfactant P20), 3.4 mL.

BIAnormalizing solution (70%), 90 mL

Ready-To-Go GenomiPhi V3 cakes, 3 Ã— 8-well strips; 2x Denaturation buffer, 1 Ã— 1.2 ml; Control DNA (Lambda), 500 ng; 12 Strips of 8 domed caps

Sample Buffer (white cap) 1 Ã— 0.5 ml; Reaction Buffer (Blue cap) 1 Ã— 0.5 ml; Enzyme mix (yellow cap) 1 Ã— 20 Âµl; Positive control DNA (pUC19, 2 ng/Âµl) 1 Ã— 50 Âµl

Series S Sensor Chip CAP, Biotin CAPture Reagent, Regeneration Stock 1, Regeneration Stock 2

Ready-To-Go GenomiPhi V3 cakes, 5 Ã— 96-well plate; 2x Denaturation buffer, 5 Ã— 1.2 ml; Control DNA (Lambda), 500 ng; 5 x 12 Strips of 8 domed caps

Single Cell GenomiPhi Reaction buffer 1 x 275 Âµl, Single Cell GenomiPhi Enzyme mix 1 x 25 Âµl, Single Cell GenomiPhi Amplification mix 1 x 25 Âµl, Single Cell GenomiPhi DTT Solution 1 x 100 Âµl, Single Cell GenomiPhi Lysis buffer 1 x 100 Âµl and Single C

Sample Buffer 1 x 0.9 ml; Reaction Buffer 1 x 0.9 ml; Enzyme Mix 1 x 100 Âµl; Control DNA (Lambda), 10 ng/Âµl 1 x 20 Âµl

Input Volume

100 Âµl solution or up to 900 mg agarose

Inhibitor

Pefabloc TH benzamidine, AEBSF, APMSF, antithrombin III, Antipain, Î±1-antitrypsin, aprotinin, chymostatin, hirudin, leupeptin, PMSF

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

Material

Cellulose Nitrate

Mixed Cellulose Ester (MCE)

Nitrocellulose (NC)

Order Information

Custom options of the product, including larger pack sizes, are available. Please contact your local sales representative or Technical Support.

Not for use with ultrasalt-sensitive automated DNA Sequencers (e.g. ALFexpress II and ABI 377 model.)

Order E. coli BL21 separately.

Custom options of the product are available. Please contact your local sales representative or Technical Support to request. Larger pack sizes available on request.

Custom options of the product are available. Please contact your local sales representative or Technical Support to request., Larger pack sizes available on request; please inquire.

Pack size

1000 reactions

480 reactions

100 mg

50 columns

500 reactions

50 pieces

500 units

1 pack

100 mL

20 columns

100 pieces

100 reactions

500 mL

1 piece

2000 reactions

90 mL

24 reaction

25 reactions

1 kit

100 purifications

Operating Temp Max

130

80ÂºC

Order code

SH30042.01

SV30031.01

SH30236.02

Porosity Max

77%

84%

Parameter

Ab Buffer Kit

Reaction Time

1.5-2 h

4-18 h depending on starting material

2 to 4 hours

4 h

Protein Binding

Medium

Sample Volume

50 Î¼l

25 to 50 Î¼l

< 100 Âµl

< 100 Î¼l

< 2.5 mL

< 1 mL

Sample weight1

1 pgâ€“10 ng of purified plasmid or M13 DNA; bacterial or M13 liquid cultures, colonies, plaques or stocks

Porosity Min

66%

74%

Sample Size

50 bp-10 kb

Sample Weight

10 ng of genomic DNA; or non-purified cell lysates

1 pgâ€“10 ng of purified plasmid or M13 DNA; bacterial or M13 liquid cultures, colonies, plaques or stocks

10 ng of genomic DNA or non-purified cell lysates

Reaction time1

16-18 h

Recognition Site

PreScission Protease

Storage

20 to 25Â°C, TE buffer (10 mM Tris/HCl, 1mM EDTA) containing 0.05% Kathon CG/ICP Biocide as preservative.

4 to 30Â°C

4Â°C, TE buffer (10 mM Tris/HCl, 1mM EDTA, pH 7.6)

Store frozen

Storage Conditions

Store the kit at ambient temperature. Once opened, completely reseal the pouch containing the Ready-To-Go GenomiPhi V3 DNA amplification kit cakes. To ensure maximum shelf life and optimal performance, storage in a desiccator is recommended. Store recons

2 â€“ 8Â°C, except Regeneration Stock 1, which may be stored at room temperature to avoid precipitatoin.

Store the kit at -70Â°C. The enzyme mix must be stored at -70Â°C; all other components may be stored at -20Â°C. Thaw components on ice and maintain at 0Â°C to 4Â°C during handling.

Store at +2 Â°C to 8 Â°C

-20Â°C

4â€“8Â°C, 20% Ethanol

Store the kit at -70Â°C. The enzyme mix must be stored at -70Â°C; all other components may be stored at -20Â°C. Thaw components on ice and maintain at 0Â°C to 4Â°C during handling.

Store the kit at -70ÂºC. The enzyme mix must be stored at -70ÂºC; all other components may be stored at -20Â°C.

Store Denature buffer and control DNA at -70Â°C or -15Â°C to -30Â°C. Store TempliPhi premix at -70Â°C.Do not store TempliPhi premix at -15Â°C to -30Â°C. Thaw the premix on ice and maintain at 0â€“4Â°C during handling. Do not allow premix to warm above 4Â°

2Â°C to 8Â°C

Store all products at +2 Â°C to 8 Â°C except BIAdesorb solution 1, which should be stored at room temperature (SDS precipitates at low temperature).

The product can be stored for 2 months or less at -20Â°C. For storage longer than 2 months, store at -70Â°C Thaw components on ice and maintain at 0â€“4Â°C during handling. DO NOT warm above 4Â°C prior to amplification

Pack size

Scale

Lab

Sample Type

PCR mixtures, enzyme reactions, DNA solutions, agarose gel slices

Pore Size

0.45

0.2

Product Size Avg

> 10 kb

>10kb

Stability

This product has been designed to deliver high quality results for up to 12 months from the manufacturing date. Please refer to the expiration date on the product label.

Stability_25

The TempliPhi premix is stable at -70Â°C for 6 months from the date of manufacture. The denature buffer and positive control DNA are stable at -70Â°C or -15Â°C to -30Â°C for 12 months from the date of manufacture

Sterility

Sterile

Storage conditions

4–8°C, 20% Ethanol

Sterilization

0.1 Âµm sterile filtered

Select Product

Reel, phenophthalein

Storage Temperature

Room Temperature

Solvent Resistance

Medium

Quantity

Ligand_7

Protein A

Membrane Type

ME 24

Plain

Unit Definition

One unit of enzyme cleaves >= 90% of 100 Î¼g of a test GST fusion protein when incubated in 1 Ã— PBS at 22Â°C for 16 h.

Weight Min

3.6 mg/cmÂ²

With Pad

Yield

PCR 10 Âµl elution volume-65%, PCR 50 Âµl elution volume-82%, 300 mg agarose 10 Âµl elution volume-57%, 300 mg agarose 50 Âµl elution volume-91%

Vector

pGEX-4T-1, pGEX-4T-2, pGEX-4T-3, pGEX-2TK

Weight Max

5.5 mg/cmÂ²

Supplied As

Lyophilized powder

Type

Reel

Discs

If you are viewing this page as a nonregistered user, the price(s) displayed is list price. To view your customer-specific pricing, log in or become a registered user here.

TEMPLIPHI - 2000 REACTION KIT

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TEMPLIPHI 100AMPLIFICATION KIT

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25640010

GENOMIPHI V2 DNA AMP KIT 25RXN

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$268.00 / EA

25660030

TEMPLIPHI 500AMPLIFICATION KIT

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25640050

POLY (A) 100 MG

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$332.00 / EA

27411001

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29357373

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29108107

ILLUSTRA EXOSTAR (100 RCN)

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ILLUSTRA EXOSTAR (2000 RCN)

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$1,347.00 / EA

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SeraSil Mag 400 5ml

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$126.03 / EA

29357369

ME24/21 ST 0.2uM 50MM 100/PK

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10406972

NAP-10 50 ST

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$514.00 / EA

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RTG GENOMIPHI HY KIT 24 RXNS

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$654.00 / EA

25-6603-24

RTG GENOMIPHI V3 KIT 480 RXNS

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$3,739.00 / EA

25-6601-97

RTG GENOMIPHI V3 KIT 24 RXNS

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$268.00 / EA

25-6601-24

THROMBIN PROTEASE

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$190.52 / EA

27084601

ME24/21 ST 0.2uM 47MM 100/PK

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$69.17 / PK

10406970

NC45 ST 0.45uM 47MM 100/PK

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$243.00 / PK

10401170

STREPTAVIDIN-HRP CONJUGATE

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$320.32 / EA

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$334.00 / EA

17085201

MICROSPIN S-300 HR, 50 COLUMNS

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$318.00 / EA

27513001

MICROSPIN S-400 HR, 50 COLUMNS

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$333.00 / EA

27514001

NAP-25 50 ST

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$712.00 / EA

17085202

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$190.24 / EA

28903059

ProbeQuantTM G-50 Micro Col,50

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$308.00 / EA

28903408

GFX PCR DNA,Gel Band Purif,100

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$266.00 / EA

28903470

PGEX-6P-1 VECTOR

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$958.16 / EA

28954648

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$1,083.60 / EA

29423383

MICROSPIN G-50 COLUMNS, 50 COLUMNS

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27533001

Fraction collector F9 T

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$8,390.40 / EA

29454032

AA DISCS 6MM/1000/PK

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$306.00 / EA

2017-006

Surfactant P20

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$303.60 / EA

BR100054

Biotin CAPture Kit Series S

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$1,524.00 / EA

28920234

PROTRAN BA85 132MM 50/PKG

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$476.00 / PK

10402525

BIACORE 8K Maintenance chemicals

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$697.20 / EA

29229054

Trypsin, 500 mL

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$42.29 / EA

SH30236.02

LM-TRYPSIN W/0.1% EDTA,100mL,AD

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$33.39 / EA

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Micro nozzle F9 R

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$407.59 / EA

29501533

46 results for : Microbiology : "Cytiva"

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