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$50~$75(0)

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$100000~$3000000(0)

Absorbance

(0.1M in H2O) 1 cm cell

(0.1 M in H2O) at 0nm

0.01 max. (0.1M solution) at 280nm

0.02 max. at 280nm

<=0.01(0.1M solution) at 260nm

Absorbance Conditions

(0.1M in H2O)

(0.1M aq. soln.)

Additional Information

Vapor Pressure: Negligible

Assay Percent Range

99+%

>=98 %

>=99 %

>=99%

Assay Percent Notes

Dry basis

CAS Registry Number 1

4432-31-9

7365-45-9

5625-37-6

145224-94-8

75277-39-3

Beilstein Reference

883043

Chemical Name or Material

HEPESsodiumsalt

HEPES

HEPES buffer soln.

Hepes Buffer

MES buffer soln.

PIPES buffer soln.

HEPES Sodium Salt

MES monohydrate

MES

ChEBI

CHEBI:42334

CHEBI:46758

CHEBI:44933

CHEBI:39005

CAS Registry Number 2

7732-18-5

Chem Alert Storage Symbol

Gray

Color

Colorless

Undesignated

White

Orange-Yellow to White

Chem AlertStorageSymbol

Gray

Concentration

1.0M buffer soln.

0.5M buffer soln.

DNase RNase Proteaseact

None detected

Concentration

0.95 to 1.05M

D Nase

DNase free

DNase

DNase free

D NaseRNaseProteaseact

None detected

EINECS Number

224-632-3

230-907-9

Formula Weight

195.24

238.197

213.25

213.2

260.27

238.3

Formula Weight

213.26

Grade

Biochemical

Molecular Biology

Health Hazard 3

P261-P271-P304+P340-P312-P501c

GHS P Statement:
Avoid breathing dust/fume/gas/mist/vapors/spray.
Wash face, hands and any exposed skin thoroughly after handling.
IF ON SKIN: Wash with plenty of soap and water.
Wear protective gloves/protective clothing/eye prote

GHS P Statement
P280-P305+P351+P338-P362-P321-P332+P313-P337+P313
Wear protective gloves/protective clothing/eye protection/face protection.
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present

Emergency Overview
Harmful if swallowed. Wear personal protective equipment. Ensure adequate ventilation. Do not get in eyes, on skin, or on clothing. Avoid ingestion and inhalation. Use personal protective equipment. Wash off immediately with

Emergency Overview
Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediate

Health Hazard 1

Warning

H335

Health Hazard 2

GHS H Statement
H315-H319
Causes skin irritation.
Causes serious eye irritation.

WARNING!

GHS H Statement:
Causes skin irritation.
May cause respiratory irritation.
Causes serious eye irritation.

Identification

Pass Test

IUPAC Name

2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid

2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid

sodium;2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate

2-morpholin-4-ylethanesulfonic acid

2-morpholin-4-ylethanesulfonic acid;hydrate

Linear Formula

OCH2CH2NCH2CH2(CH2CH2SO3H)

InChIKey

IHPYMWDTONKSCO-UHFFFAOYSA-N

JKMHFZQWWAIEOD-UHFFFAOYSA-N

RDZTWEVXRGYCFV-UHFFFAOYSA-M

SXGZJKUKBWWHRA-UHFFFAOYSA-N

MIIIXQJBDGSIKL-UHFFFAOYSA-N

Merck Index Reference

15, 4689

15, 5972

Infrared Spectrum

Authentic

MFCD Number

MFCD00006158

MFCD00006159

MFCD00006181

MFCD00036463

MFCD00149409

MFCD00283848

Molecular Formula

C6H15NO5S

C8H18N2O6S2

C6H13NO4S

C8H18N2O4S

C8H17N2NaO4S

Molecular Weight

302.36

195.233

213.248

260.284

Packaging

Glass Bottle

Plastic bottle

Poly Bottle

Percent Purity

≥99%

>=98%

>=99%

0.996

5.0 to 15.0 (1% soln.)

8.0

2.5 to 4.0 (1%, H2O, 25°C)

3.5 to 4.1

5.0 to 6.5

6.0

7.5

7.2 to 7.5

Solubility

Solubility in water: soluble.

Solubility in water: 10g/100mL.

Solubility Information

Fully misicible in water. Soluble in water (0.05 M),giving a clear,colorless solution (the resulting solution has a pH of approximately 4.5-4.7). 10 g will dissolve in 40 mL of 0.1 N NaOH.

Miscible with water.

Soluble in water (0.5 M at 20°C).

Synonym

2-(N-Morpholino)ethanesulfonic acid

2-(4-Morpholino)ethane Sulfonic Acid

N-(2-Hydroxyethyl)piperazine-N«-2-ethanesulfonic Acid

N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic Acid

2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid sodium salt

N-[2-Hydroxyethyl]piperazine-N'-[2-Ethanesulfonic acid]

2-(N-Morpholino)ethanesulfonic acid monohydrate

2-[N-morpholino]ethanesulfonic acid

Name Note

99+%

1M Solution, pH 7.3

Pub Chem CID

78165

23831

16218417

2724248

79723

Quantity

250mL

1kg

100mL

50g

100g

500mL

500g

Recommended Storage

Ambient temperatures

Keep cold

p Ka

5.89 to 6.29 (at 25°C)

7.3 to 7.7 (at 25°C)

Purity GradeNotes

DNase-, RNase- and Protease-Free

DNase- and RNase-Free

Physical Form

Crystalline Powder

Powder, Crystals or Flakes

Liquid

Powder/Solid

Powder

Melting Point

~236°C (decomposition)

300°C

>300.0°C

248.0°C

SMILES

C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

C1COCCN1CCS(=O)(=O)O.O

C1CN(CCN1CCO)CCS(=O)(=O)[O-].[Na+]

C1CN(CCN1CCO)CCS(=O)(=O)O

C1COCCN1CCS(=O)(=O)O

Solubility

Soluble in water

Moisture

7.9 to 9.1%

RTECS Number

TL6809000

Molecular Weight

238.302

T LC

Single spot

Protease

Protease free

Water

8.0 to 9.0% (K.F.)

1% max.

3% max. (K.F.)

TSCA

Yes

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