Government Scientific Source

Account

User Name/Email address

Password

Trouble Logging In?

Welcome Back, !

Account Settings

Profile Settings
Payment Methods
Address Book

Orders & Tools

Order History & Details
Quotes & Inquiries
Credit Card Reconciliation
Favorites
Saved Carts
Quick Order

Logout

Forgot

Password

Cart ⮟

Continue to Checkout
Your cart is empty.

HOME
SUPPLIERS
SPECIAL OFFERS
COVID-19 IN STOCK NOW PROMOTIONS CLEARANCE
ORDERS
CREDIT CARD HISTORY QUICK ORDER ITEM REQUEST QUOTE RETURNS
CONTACT US

Search results for : Liquid chromatography LC columns

FILTER PRODUCTS BY

In Stock

Covid-19 Items

Made in USA

Chromatography

Chromatography Consumables

Chromatograph Columns

Liquid chromatography LC columns

Agilent Technologies(126)

Antylia (Formerly Cole Parmer)(2)

Bio-Rad(47)

Cytiva(125)

DWK Life Sciences Inc(3)

Fisher Scientific(4)

Kemtech America Inc(1)

Macherey-nagel(23)

Metrohm USA(2)

MilliporeSigma(6)

Mine Safety Appliances(1)

National Scientific(3)

PERKINELMER US LLC(6)

Phenomenex(25)

Restek(2)

Thermo Laboratory Equipment Division(1)

Thomson Instrument Company(3)

Waters(3)

Enter your price range below

Min

Max

$0~$10(1)
$10~$25(0)
$25~$50(3)
$50~$75(3)
$75~$100(14)
$100~$200(27)
$200~$300(63)
$300~$400(15)
$400~$500(19)
$500~$750(58)
$750~$1000(58)
$1000~$5000(117)
$5000~$10000(5)
$10000~$50000(0)
$50000~$100000(0)
$100000~$3000000(0)

$0~$10(1)

$10~$25(0)

$25~$50(3)

$50~$75(3)

$75~$100(14)

$100~$200(27)

$200~$300(63)

$300~$400(15)

$400~$500(19)

$500~$750(58)

$750~$1000(58)

$1000~$5000(117)

$5000~$10000(5)

$10000~$50000(0)

$50000~$100000(0)

$100000~$3000000(0)

Application

Can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design

GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function

Purification is done under physiological conditions and mild elution preserves the activity of the target protein

Highly suitable for preparative purification of histidinetagged recombinant proteins

Designed for use with a syringe, peristaltic pump, or chromatography system

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

They can be used for any DNA greater than 10 bases in length and are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction

For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

Purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®

Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins

Average sample loading capacity

50 mg

Bed Dimensions

16 x 25 mm

26 x 600 mm

7 x 25 mm

16 x 100 mm

16 x 600 mm

0.7 x 2.5 cm

1.6 x 2.5 cm

Bed Height With One Adaptor

85 mm-109 mm

140-280 mm

230 mm-350 mm

810 mm-950 mm

225 mm-350 mm

525 mm-650 mm

579 mm-609 mm

129 mm-159 mm

185 mm-209 mm

825 mm-950 mm

0 mm-29 mm

Bed Height With Two Adaptors

80 mm-350 mm

96 mm-156 mm

85 mm-350 mm

0 mm-26 mm

158 mm-206 mm

0 to 200 mm

0-280 mm

680 mm-950 mm

58 mm - 106 mm

380 mm-650 mm

546 mm-606 mm

650 mm-950 mm

Bed Volume

120

1 ml TALON® Superflow /column

320

5 mL

1 mL

Application_4

Chromatography system|Manual

Applications

LPLC: suitable

Bed Height

600 mm

100 mm

25 mm

Bed Support

Filter

Net

Binding Capacity/ml Chromatography Medium

At least 40 mg histidine-tagged protein/mL resin

~ 13 mg ovalbumin

Approx. 35 mg human lgG/mL at 2.4 min residence time

Approx. 40 mg histidine-tagged protein

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

up to 15mg histidine-tagged protein

125 mg human IgG/column

Avoid Using

Chelating agents such as EDTA, EGTA , citrate

Oxidizing agents, citrate buffers

Capacity

1.1 meq/mL by wetted bed volume

BioProcess resin

yes

Bore Size (mm)

CAS_NO

69011-20-7

9052-95-3

Certificate of Analysis

Yes

Chemical Stability

For Ni²⁺-stripped medium 0.01 M HCl, 0.1 M NaOH. Tested for one week at 40°C 1 M NaOH, 70% HAc. Tested for 12 h 2% SDS. Tested for 1 h 30% 2-propanol. Tested for 30 min

Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol

Commonly used aqueous buffers,1 M sodium hydroxide6, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to acetone, ketones, chlorinated hydrocarbons, aliphatic esters, phenol, NaOH, >10% HCl, >5% acetic acid, or strong mineral acid|å¯èinå—æ¶²ç›¸å±‚æžä½¿ç”¨çš,æ°´æin

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

Stable in aqueous buffers commonly used in Protein A Chromatography

The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol

Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea

Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons

Chemical Substance ID

24870506

Chromatography technique

Ion Exchange Chromatography

Hydrophobic Interaction Chromatography

Histidine-tagged protein purification

Antibody affinity chromatography

GST-tagged protein purification

Group specific affinity

Column i.d.

26 mm

10 mm

16 mm

50 mm

5 mm

Column Capacity

< 1 mg/mL

66 mL

Column volume

1 mL

Description

Omnifit EZ Replacement Glass, 6.6 x 50mm; 1/ea

Diameter Metric Inner

26 mm

13 mm

6.6 mm

Flow Rate

0.8-3.9 ml/min

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

< 31 ml/min

< 4 ml/min

< 4 ml/min|< 4 ml/min

< 15 ml/min

5 mL/min - 20 mL/min

0.5 ml/min - 2.5 ml/min

1 mL/min|1 mL/min

4 ml/min (1 ml), 5 ml/min (5ml)|4 ml/min (1 ml), 5 ml/min (5ml)

< 20 ml/min|< 20 ml/min

1 mL/min - 4 mL/min

< 20 mL/min

4 mL/min (1 mL), 5 mL/min (5ml)

5 mL/min|5 mL/min

Recommended: 0.5 to 2.0 mL/min

> 20 ml/min|> 20 ml/min

1.6 mL/min

< 1 ml/min

< 2.6 ml/min

1.2 - 2.3

2 ml/min - 10 ml/min

Flow Velocity

< 150 cm/h

Up to 40 cm/h

â‰¤ 600 cm/h

15 cm/h

< 300 cm/h

< 500 cm/h|< 500 cm/h

< 450 cm/h

< 300 cm/h|< 300 cm/h

10 cm/h - 50 cm/h

100 cm/h - 300 cm/h 1

15 cm/h|15 cm/h

< 600 cm/h

For Use With

HiTrap and HiPrep columns (connecting column to AKTAdesign systems).

The volumes are designed for Biacore 8K+, Biacore 8K but the kit can be used with all Biacore SPR systems

PD-10 Desalting Columns

Connect a syringe to the top of a HiTrap columns.

For Use With Equipment

420570 Series reservoir and 420575 series flow control adapters

Omnifit EZ and EZ SolventPlus Columns

Format_6

Prepacked column

Kits

Prepacked columns

Bulk resin

Formula

[CH₂CH(C₆H₅)]_x[CH₂CH[C₆H₄

For Use With Application

High resolution size exclusion chromatography (SEC) in micro scale (4 to 50μL sample volume)

Application_5

Chromatography system|Manual

H-Codes

H319

Hold-up Volume of Membrane

<20 Î¼l

<10 Î¼l

<5 Î¼l

COLOR

Blue

Diameter Metric Outer

32 mm

Filtrate Vessel Material

Polycarbonate (PC)

Inlet id

0.75 mm (0.029 in)

1 mm (0.04 in)

1.55 mm (0.06 in)

1.0 mm (0.04 in)

2 mm (0.08 in)

Includes

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Dismantling tool, support screen, net ring (10 Âµm), O-ring, stop plugs, and instructions.

1 Ã— Column stand 4 Ã— PD-10 spin adaptor 1 Ã— Buffer tray 30 Ã— Bottom sleeve Instructions are supplied with the columns

Instructions supplied with the concentractors

Ligand_7

2-mercaptopyridine

Protein A, alkali stablilized

Protein A

Protein A, alkali stabilized

Ligand

Octylamine

Alkali-stabilized Protein A-derived (E. coli)

Recombinant protein A (E.coli)

Glutathione

Matrix

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

Highly cross-linked agarose, spherical

cross-linked agarose, 4%, spherical

Highly cross-linked agarose

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Ion exchanger

S (cation exchanger)

Includes

Fritted disc, 2mm bore PTFE stopcock plug and 24/40 standard taper upper joint

Matrix

6% Cross-Linked Agarose

IOD-Codes

Ionic Capacity

40-85 Âµmol/ml (Cl^-)

Material

Stainless Steel

Polypropylene (PP)

Glass

Polycarbonate (PC)

Kit Contents

Anti-histidine antibody: 1 mg/ml in 0.15 M NaCl, 90 Î¼l
Immobilization buffer: 10 mM sodium acetate pH 4.5, 2.6 ml
Regeneration solution: 10 mM glycine-HCl, pH 1.5, 2 Ã— 120 ml

Length Metric

457 mm

Molecular Weight

0.0

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

Length Metric

500 mm

50 mm

Ligand Coupling Method

Epoxy activation

Length English

18 in.

Ligand Density

6 mg rProtein A/ml medium

MDL Number

MFCD00146442

MFCD00132726

Order Information

HiLoad columns are prepacked XK columns

Allows safe, reliable storage and transport of self-packed Tricorn columns.

Columns included in HiTrap HIC Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

Disposable columns for clean-up of proteins/oligosaccharides. The columns are prepacked with Sephadex G-25 resin and for sample volumes up to 2.5 mL by gravity flow and columns spin flow. PD- 10 Buffer reservoir has to be ordered separately For smaller s

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

This product is produced based on a customer order. Delivery time may be longer than for standard products.

HiLoad columns are prepacked XK columns.

Not suitable for use with AKTAprime plus system.

Columns included in HiTrap IEX Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

The use of these products in Biacore systems requires an Amine Coupling Kit with Sensor Chip CM5, CM4, CM3, or C1.

Pack size

200 mL

25 mL

4 x 53 mL

1 Ã— 5 mL

50 Ã— 100 ÂµL

50 columns

7 Ã— 1 mL

4.7 mL

5 Ã— 5 mL

100 mL

20 columns

30 columns

5 mL

53 mL

1 Ã— 120 mL

1 piece

20 mL

1 Ã— 320 mL

1 kit

5 Ã— 1 mL

Particle size, d_50V

~85 Âµm

~90 Âµm

~34 Âµm

~34 Î¼m

Metal Ion Capacity

approx. 15 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

pH Stability Working Range

3â€“12

pH stability, CIP

2â€“14

2â€“13.7

3â€“12

2â€“10.5

Porosity

20 Î¼m

7 Î¼m

10 Î¼m

10 or 80 Âµm

Pressure/flow characteristics

150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)

Operating Flow Rate, Recommended

1.3 mL/min

0.5 mL/min

Particle Size

60 Âµm-165 Âµm

45 Âµm-165 Âµm

pH stability, operational

4â€“9

3â€“10

3â€“12

3â€“13

PoreSize

40 to 60 µm

Pack Size

Pictogram

GHS07

Pressure/Flow Specification

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

Operating Flow Rate, Max

1.0 mL/min

2.6 mL/min

Outlet id

0.75 mm (0.029 in)

1.0 mm (0.04 in)

1.55 mm (0.06 in)

Pore Size

40 to 60 µm

Sample Volume

< 0.5 mL

< 5 mL

600 Âµl

<= 15 ml

<= 5 ml

2.5 ml

<= 13 ml

100-180 Î¼l

<= 1.5 ml

0.1 to 0.5 ml (Gravity), 0.2 to 0.5 ml (Spin)

Operating Temperature

4Â°C-60Â°C

Signal Word

Warning

Pressure Limit

20 bar (2 MPa, 290 psi)

Resin Type

Sepharose Fast Flow

MabSelect

Sepharose High Performance

RIDADR

NONH for all modes of transport

R-CODES

36/37/38

Storage

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

+4Â°C to +8Â°C

4 to 30Â°C

4 to 30Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

4 to 30Â°C, 0,05% Kathon CG/ICP Biocide

4 to 30Â°C, 0.15% Kathon CG

2Â°Câ€“8Â°C, 20% ethanol

4 to 30Â°C, in 20% ethanol

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

4 to 30ËšC

4Â°C to 8Â°C

4 to 30Â°C, 20% Ethanol or 70% Acetonitrile

2 to 8Â°C, 20% Ethanol

4 to 30Â°C, 0.05 M Sodium Acetate containing 20% Ethanol

4â€“30Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

4 to 30Â°C, 20% Ethanol (Q, DEAE, ANX,CM), 20% Ethanol, 0.2 M Sodium Acetate (SP)

4Â°C to 30Â°C, 20% Ethanol

Scale

Lab

Lab|Process development|Process

Lab|Process development

Lab|Process

Storage Conditions

Store at 2Â°C to 8Â°C

4 - 30Â°C

Recommended Flow Rate

1 ml/min

Quantity

Volume

100-500 Âµl

5-20 ml

2-6 ml

400 Âµl - 2 ml

Size MetricStopcock

2 mm bore

Wetted Part

yes

Type

Liquid Chromatography (LC)

Chromatography Column

Gravity Chromatography Column

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Theoretical Plates

5000 m-1

> 13000 m-1

Volume_28

400 Âµl - 2 ml

2-6 ml

100-500 Âµl

5-20 ml

P-CODES

P305 + P351 + P338

Volume Metric Bed

≈2.4mL

Volume MetricBed

≈2.4mL

Storage Requirements

4° to 30°C

Total binding capacity

~50 mg hIgG/mL resin

S-Codes

WGK

If you are viewing this page as a nonregistered user, the price(s) displayed is list price. To view your customer-specific pricing, log in or become a registered user here.

HISTRAP HP 5 X 5 ML

Cytiva

$899.13 / EA

17524802

COLUMN PD 10

Cytiva

$430.99 / EA

17085101

5183-4511 | Clear snap cap slit PTFE Si septa 100PK

Clear snap cap slit PTFE Si septa 100PK

Agilent Technologies

$58.15 / EA

5183-4511

7311553 | Poly Prep Chromatography Columns pk 1000

Poly Prep Chromatography Columns pk 1000

Bio-Rad

$2,171.52 / EA

7311553

XK 26/40 column

Cytiva

$1,157.03 / EA

28988949

StrepTrap XT 1x5 ml

Cytiva

$710.95 / EA

29401322

StrepTrap XT 5x1 ml

Cytiva

$724.88 / EA

29401320

HITRAP PHENYL HP, 5 X 1ML

Cytiva

$322.95 / EA

17135101

HITRAP CHELATING HP 5 X 5 ML

Cytiva

$956.05 / EA

17040903

HITRAP SP HP, 5X5 ML

Cytiva

$583.16 / EA

17115201

HIPREP 26/10 DESALTING

Cytiva

$978.12 / EA

17508701

HITRAP Q FF, 5 X 1 ML

Cytiva

$217.24 / EA

17505301

HiTrap 5x1 ml MabSelect SuRe pcc

Cytiva

$1,392.83 / EA

17549111

HISTRAP HP 1 X 5 ML

Cytiva

$228.84 / EA

17524801

GSTRAP HP 5 X 1 ML

Cytiva

$526.23 / EA

17528101

GSTRAP HP 5 X 5 ML

Cytiva

$2,058.48 / EA

17528202

NI SEPHAROSE 6FF, 100 ML

Cytiva

$1,539.20 / EA

17531802

NAP-5 50 ST

Cytiva

$483.00 / EA

17085302

HITRAP IMAC HP, 5 X 1 ML

Cytiva

$277.63 / EA

17092003

GSTRAP FF, 5X1ML

Cytiva

$476.28 / EA

17513001

HITRAP SP FF, 5 X 1 ML

Cytiva

$217.24 / EA

17505401

HIPREP 16/60 SEPHACRYL S-300 HR

Cytiva

$1,059.44 / EA

17116701

MBPTrap 5 x 1mL

Cytiva

$469.32 / EA

28918778

Vivaspin 500, 50 kDa MWCO PES

Cytiva

$167.80 / EA

28932236

Vivaspin 6, 5 kDa MWCO PES

Cytiva

$233.47 / EA

28932294

Vivaspin 20, 50 kDa MWCO PES

Cytiva

$218.28 / EA

28932362

StrepTrap HP 5 x 1mL

Cytiva

$1,101.60 / EA

28907546

HiScale 50 20BH

Cytiva

$2,693.91 / EA

28964445

Vivaspin 6, 3 kDa MWCO PES

Cytiva

$233.47 / EA

28932293

Vivaspin 20, 100 kDa MWCO PES

Cytiva

$218.28 / EA

28932363

HISTRAP FF CRUDE 5X1 ML

Cytiva

$259.05 / EA

11000458

HITRAP PROTEIN G HP, 5 X 5 ML

Cytiva

$3,860.23 / EA

17040503

HITRAP PROTEIN A HP,1 X 5 ML

Cytiva

$913.07 / EA

17040301

HITRAP PROTEIN A HP, 5 X 5 ML

Cytiva

$4,062.35 / EA

17040303

HiTrap Heparin HP 5x5ml

Cytiva

$1,330.11 / EA

17040703

HITRAP CHELATING HP, 1 X 5 ML

Cytiva

$246.28 / EA

17040901

RPROTEIN A SEPHAROSE FF 5 ML

Cytiva

$853.81 / EA

17127901

HITRAP DEAE FF 5 X 1 ML

Cytiva

$217.24 / EA

17505501

STREPTAVIDIN SEPHAROSE HP,5 ML

Cytiva

$713.27 / EA

17511301

GSTRAP FF, 1X5ML

Cytiva

$462.35 / EA

17513101

383 results

Contact the GSS Team Today!
GSS-Sales@govsci.com | 800.248.8030 | www.govsci.com

Government Scientific Source is a leading distributor of laboratory products and equipment dedicated to serving federal, state, and local governments.

ISO 9001:2015 Certified

to our newsletter to stay in touch with our latest deals.

Account Settings

Orders & Tools

Category

Supplier

Price

Attributes