Government Scientific Source

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Bed Dimensions

26 x 600 mm

16 x 600 mm

Bed Volume

120

320

4 to 6mL/g in Distilled Water

Bed Height

600 mm

BioProcess resin

yes

Certificate of Analysis

Yes

Chemical Stability

Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Column i.d.

26 mm

16 mm

Diameter Metric Inner

2.6cm

Flow Rate

< DarcyÂ´s Law

>100cm/hr.

Format_6

Prepacked column

Bulk resin

Flow Velocity

15 cm/h|15 cm/h

Format

Solid Spherical Bead

Height English

11.81 in.

Height Metric

30cm

For Use With Application

Desalting and Buffer exchange and removal free label in industrial applications

Matrix

Composite of cross-linked agarose and dextran

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Cross-linked dextran

Max Pressure

>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

pH stability, CIP

1â€“14

2â€“13

Operating Flow Rate, Max

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.35 mL/min water at low temperature;
0.17 mL/min 20% ethanol or 10% glycerol at low temperature

1.0 mL/min

2.6 mL/min

Partical Size Dry

20 Âµm-80 Âµm

pH stability, operational

2â€“10

3â€“12

3 to 12

3â€“11

Operating Flow Rate, Recommended

1.3 mL/min

0.5 mL/min

0.45 mL/min

Pack size

750 mL

150 mL

100 g

Particle Size

24 Âµm-44 Âµm

38 to 235µm in wet form, >50µm in dry form

pH Stability Cleaning-in-Place (CIP)

1 to 14

Particle size distribution, wet

34â€“208 Âµm

Particle size, d_50V

~34 Âµm

~ 8.6 Âµm

~50 Âµm

Pressure/Flow Specification

min 150 cm/h, bed height 10 cm, column 5 cm i.d.

pH Range

2 to 13

Product Type

Sephadex G-25 Medium, 100 g

Quantity

100g

Storage

4 to 30Â°C

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

4Â°C to 30Â°C, 20% Ethanol

Sample Volume

<= 5 ml

<= 13 ml

4 to 50 ÂµL

Pressure/flow characteristics

Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).

Storage Requirements

4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH

Storage Conditions

4ËšC to 30ËšC, 20% ethanol

Support Type

Cross-linked Dextran

Theoretical Plates

5000 m-1

> 42 000 m^-1

Typical Pressure Drop Over the Packed Bed

~ 3 MPa

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9 results

Contact the GSS Team Today!
GSS-Sales@govsci.com | 800.248.8030 | www.govsci.com

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