Government Scientific Source

$0~$10(0)
$10~$25(2)
$25~$50(3)
$50~$75(1)
$75~$100(16)
$100~$200(16)
$200~$300(22)
$300~$400(17)
$400~$500(10)
$500~$750(17)
$750~$1000(8)
$1000~$5000(14)
$5000~$10000(0)
$10000~$50000(1)
$50000~$100000(2)
$100000~$3000000(1)

$0~$10(0)

$10~$25(2)

$25~$50(3)

$50~$75(1)

$75~$100(16)

$100~$200(16)

$200~$300(22)

$300~$400(17)

$400~$500(10)

$500~$750(17)

$750~$1000(8)

$1000~$5000(14)

$5000~$10000(0)

$10000~$50000(1)

$50000~$100000(2)

$100000~$3000000(1)

Additives

With: Porcine trypsin, HBSS, EDTA Without: Calcium magnesium

Application

Chemiluminescent detection in Western, Southern, and Northern blots.

Western, Southern and Northern Blotting

Binding Capacity

162-180 µg IgG/cm²

150-176 µg IgG/cm² (incubation method) 20-72 µg BSA/cm² (incubation method)

115-125 µg IgG/cm² (incubation method) 11-43 µg BSA/cm² (incubation method)

Assay Range

31.20 to 2000 pg/mL

CAS_NO

9007-43-6

9048-46-8

26628-22-8

9004-34-6

124-20-9

Cell Permeability

Cell-Impermeant

Cell-Permeant

Chemical Reactivity

Thiol

Chemical Substance ID

329747679

329748616

Content AndStorage

Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane
• Luminol/Enhancer, 50 mL
• Stable Peroxide Buffer, 50 mL

Store reagents at 4°C.

Upon receipt store kit at room temperature.

Upon receipt store product at 4°C. Product is shipped at ambient temperature.

• Substrate Solution, 50 mL
• Stable Peroxide Solution, 50 mL

Sufficient for 12 mini-gel blots; 1000 cm2 of membrane.

Store reagents at 2–8°C.

Storage: Upon receipt store at room temperature or 4°C. Product shipped at ambient temperature.

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area.

Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction

Stable for one year at 4°C or six months at room temperature. Product is shipped at ambient temperature.

Store DAPI solution (1 mg/mL) at 2-8°C protected from light.

Sufficient for 25 mini-gel blots; 2000 cm2 of membrane
• SuperSignal West Pico PLUS Luminol/Enhancer, 100 mL
• SuperSignal West Pico PLUS Stable Peroxide, 100 mL

Store at room temperature.

Upon receipt store product at room temperature. After opening, store product at 4°C.

• Substrate Solution, 10 mL
• Stable Peroxide Solution, 10 mL

Sufficient for two mini-gel blots; 200 cm2 of membrane.

Store reagents at 2–8°C.

Store at 4°C.

Upon receipt store at -20°C protected from moisture and light. Product is shipped at ambient temperature.

Storage: Upon receipt store at 4°C, protected from moisture. Product is shipped at ambient temperature.

Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane
• Luminol/Enhancer, 50 mL
• Stable Peroxide Buffer, 50 mL

Store at room temperature.

Upon receipt store at -20°C. Product is shipped at ambient temperature.

Storage: Upon receipt store at 4°C protected from light. Product is shipped at ambient temperature.

Sufficient For: 400 mL of typical 1X TMB buffer (4000 wells at 100 µL per well)
• TMB Solution, 200 mL
• Peroxide Solution, 200 mL

Store at 4°C

Store between 2°C and 8°C.

Sufficient For: 25 mini-gel blots; 2000 cm2 of membrane
• Luminol/Enhancer, 100 mL
• Stable Peroxide Buffer, 100 mL

Store reagents at 4°C.

Upon receipt store at room temperature. Product shipped at ambient temperature.

Upon receipt store product 4°C. Product is shipped at ambient temperature.

Cross Reactivity

<50% cross-species reactivity observed with species tested. <0.5% cross-reactivity observed with available related molecules.

Content And Storage

Store at 4°C.

Store between 2°C and 8°C.

Store membranes flat at ambient temperature, away from chemical vapors. Some solvent vapors may partially dissolve the membranes, which will disrupt the pore structure. Keep membranes out of direct sunlight. Sunlight may cause the nitrocellulose to dry an

Sufficient for 2 mini-gel blots; 200 cm2 of membrane
• SuperSignal West Pico PLUS Luminol/Enhancer, 10 mL
• SuperSignal West Pico PLUS Stable Peroxide, 10 mL

Store at room temperature.

Upon receipt store at -20°C. Product is shipped with an ice pack.

Upon receipt store solutions at 4°C Products are shipped at ambient temperature.

Sufficient For: 2 mini-gel blots; 200 cm2 of membrane
• Luminol/Enhancer, 10 mL
• Stable Peroxide Buffer, 10 mL

Store reagents at 4°C.

Sufficient For: 25 mini-gel blots; 2000 cm2 of membrane
• Luminol/Enhancer, 100 mL
• Stable Peroxide Buffer, 100 mL

Store at room temperature.

Upon receipt store product at 4°C. Product is shipped at ambient temperature.

Conjugate

Biotin

Description

Blocker BSA in TBS (10X)

Blocker BSA in PBS (10X)

Pierce 1-Step Turbo TMB-ELISA Substrate Solution

Blocker Casein in PBS

Substrate Solution, 200mL Stable Peroxide Solution

Pierce Protein A

Pierce OPD Tablets

Pierce 1-Step NBT/BCIP Substrate Solution

Pierce Diethanolamine Substrate Buffer, 5X

Pierce Nitrocellulose Membrane, 0.45um, 30cm x 3.5m

Protein-Free (PBS) Blocking Buffer

Pierce 1-Step Ultra TMB-ELISA Substrate Solution

Pierce Recombinant Protein A/G

Description

Pierce Bovine Serum Albumin, Biotinylated

DetectionMethod

Fluorescent

Chemiluminescent

Colorimetric

Detection Method

Chemiluminescent

Colorimetric

Dimensions LxW

30 cm x 3.5 m

Dimensions_29

15 x 15 cm (5.9 x 5.9 in)

20.3 Ã— 25.4 cm (8 Ã— 10 in)

200mmx4m

150mmx4m

Dimensions_1

15 x 15 cm (5.9 x 5.9 in)

200mmx4m

20.3 Ã— 25.4 cm (8 Ã— 10 in)

150mmx4m

Dimensions_33

150mmx4m

200mmx4m

20.3 Ã— 25.4 cm (8 Ã— 10 in)

15 x 15 cm (5.9 x 5.9 in)

EC Number

232-700-9

232-674-9

Dimensions

15 x 15 cm (5.9 x 5.9 in)

200mmx4m

20.3 x 25.4 cm (8 x 10 in)

Excitation Emission

360⁄460

361⁄497

For Use With Application

ELISA

Form

Liquid

For Use With Equipment

Fluorescence Microscope, High Content Instrument

Format

Liquid

Formulation

100% nitrocellulose paper

Gene ID Entrez

3627

Guidance

Stability: The components of this product are stable until expiration date printed on box when stored under recommended conditions.

14.5 x 21.5 cm x 0.83 mm

1 x Mains cable 115 V 1 x Mains cable 220 V Instructions supplied with the power supply Adaptor 18-1129-58 required for connecting 2 mm high-voltage leads on gel units to power suppliers with 4 mm output jacks.

Storage: Room temperature, maximum 20Â°C, without any contact with heat and radioactive sources. The film can also be stored at 4Â°C

Material: Paper Dimension: 104x253 mm

Grade

GB003

Height

95 mm

COLOR

Blue

Includes

Solution A (luminol solution, 50 ml) and Solution B (peroxide solution, 50 ml), sufficient for 1000 cmÂ² membrane.

Nytran SPC, wicks and blotter stacks for five transfers from indicated gel size.

Kit Contents

SuperSignal West Pico PLUS Luminol/Enhancer, 10mL

SuperSignal West Pico PLUS Stable Peroxide, 250mL

Stable Peroxide Buffer, 50mL

SuperSignal West Pico PLUS Stable Peroxide, 100 mL

Luminol/Enhancer, 50mL

Luminol/Enhancer, 100mL

Luminol/Enhancer, 10mL

Labelor Dye

Streptavidin

MDL Number

MFCD00008229

MFCD00130890

MFCD00081512

MFCD00132176

MFCD00003536

Material

The main components in the EPS 301 power supply are made of the following materials Instrument box: Aluminium (Al) Display holder: Polypropene (PP). UL94V0 Keyboard: Polyester (P) Output sockets: Acetalplastic (POM). UL94V0 Rubber feet: Polyurethane (PP)

Cellulose

Nitrocellulose

Nitrocellulose (NC)

Label Type

Streptavidin

Biotin & Analogs

Molecular Weight

0.0

Mains Requirement

100 to 120 V/220 to 240 V; 50/60 Hz

Output Resolution

Voltage: 1 V Current: 1 mA

Membrane Area

11 x 14 cm

Power

80 W

Pack size

96 wells

For 1 000cmÂ² membrane

50 sheets

For 2 000cmÂ² membrane

For 2500 cmÂ² membrane

100 mL

For 1000 cmÂ² membrane

For detecting 1000 cmÂ²

For 3 000cmÂ² membrane

100 sheets

Operating Pressure Max

106 kPa, maximum altitude of 2000 m

Power Consumption

120 W

Output Protection

Fully protected against any overload conditions

Pack Size

500

Operating Temp Max

40Â°C

PoreSize

0.45 micron

Quantity

1 L

200 mL Kit

200 mL

25 mg

20 mL

50 mL Kit

1 Kit

100 mL Kit

20 mL Kit

1 mL

400 mL Kit

250 mL Kit

500 mL

Operating Temp Min

4Â°C

Reagent Type

Fixation Reagent

Substrate Buffer

Blocking Reagent

Recommended AntibodyConcentrations

2?: 1:20-100K (10-50ng/mL), 1?: 1:1K (0.2- 1µg/mL)

2?: 1:50-250K (4-20ng/mL), 1?: 1:5K (0.02- 1µg/mL)

2?: 1:100-500K (2-10ng/mL), 1?: 1:5K (10- 200ng/mL)

Recommended Membrane

PVDF (Amersham Hybond P) or nitrocellulose membranes (Amersham Protran)

Nitrocellulose, PVDF membranes

PVDF (Amersham Hybond P) or nitrocellulose (Amersham Protran Premium)

Recovery After Power Failure

The program continues automatically

Output Connector Type

4 mm

Sensitivity

38.4 pg/ml [Non-acetylation]

For detection of low to very low abundant proteins

For detection of low and medium abundant proteins

For detection of medium to high abundant proteins

Sensitivity

Mid-femtogram

Low to mid-femtogram

Low picogram to high femtogram

Product Type

ELISA Development Kit

Operating Humidity

0-0.95

Programming Range

Voltage: 5-300 V DC Current: 10-400 mA Time: 00.01-23.59 h or off (- - - -)

Signal Duration

6 to 8 hr

24 hr

6-24 hr

Product Line

SuperSignal

Pierce™

Pierce

Restore

Blocker

NeutrAvidin

1-Step

Shipping Condition

Room temperature

Wet Ice

Protocol Length

5 h protocol

Quantity

10 x 1 mL

10 x 10 ml

100 mL

1 Roll

250 mL

Physical State

solid

Pore Size

0.45

0.2

Species

Human

Substrate

HRP (Horseradish Peroxidase) Substrate

SubstrateType

Beta-Gal Substrate

HRP (Horseradish Peroxidase) Substrate

Storage Requirements

Store the unopened product at 2°C to 8°C. Do not use past expiration date.

Room Temperature

Substrate Type

HRP (Horseradish Peroxidase) Substrate

AP (Alkaline Phosphatase) Substrate

Sufficient For

At least 120 mini-gel blots when cut to 8 x 10 cm

Sterilization

0.1 Âµm sterile filtered

Substrate Properties

Chemical Substrate

Programming Resolution

Voltage: 1 V Current: 1 mA Time: 1 min

Solubility

ECF substrate dilution buffer: Soluble in cold water and hot water

Detection reagent 1: Soluble in cold water and hot water Detection reagent 2: Soluble in cold water and hot water Peroxidase labelled anti-mouse antibody: Soluble in cold water, hot water, methanol, acetone Peroxidase labelled anti-rabbit antibody: Solubl

Solubility

Water

Reactive Moiety

Maleimide

Storage

Store frozen

Storage Conditions

2 - 8Â°C

18 - 30Â° C

Sub CellularLocalization

Nucleus

Weight

Wetted Part

Target Molecule

Antibodies

Membrane

Nitrocellulose Membrane

Voltage_27

5-300 V

Order code

SH30236.01

Packaging Quantity

5 Plates

Type

Sheets

Width

250

Validated Application

ELISA, Western Blot

ELISA, Immunohistochemistry, Western Blot

Timer Operation

1 min to 24 h - continuous

Voltage

5-300 V

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