Bacteria genomicPrep Mini Spin Kit, 250
For the rapid extraction and purification of highquality genomic DNA from grampositive and gramnegative bacteria.
Total DNA extraction time under 1 h with reduced lysis time.
Can be used to purify 4 to 8 µg of genomic DNA from 2 × 1NA? bacteria cells with input amounts ranging from 1 × 1NA? to 4 × 1NA? bacteria cells.
Spin columns, prepacked with a silica membrane, provide excellent reproducibility and consistency.
Verified in several downstream applications including RT PCR, long PCR, and restriction enzyme digests.
Colorcoded caps for ease of use.
illustra bacteria genomicPrep Mini Spin Kit can be used to isolate genomic DNA from a wide range of bacterial strains such as DH5a, TOP1NA, JM1NA9, Bacillus subtilis, etc. The kit produces highly pure and intact genomic DNA with A26NA/A28NAvalues of 1.75 to 1.85. DNA at concentrations suitable for most downstream applications including restriction enzyme digestion, RTPCR, and long PCR. The method uses a lysis solution in combination with proteinase K to gently release genomic DNA into solution from bacteria cells. The DNA is deproteinated in the extraction solution and the genomic DNA is then bound onto a silica column in the presence of chaotropic solution. Contaminants are removed during wash steps and the purifed DNA is eluted with prewarmed TE solution.