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Chromatography

Chromatography Consumables

Reagents and Buffers

Chromatography Media

$0~$10(0)
$10~$25(0)
$25~$50(182)
$50~$75(52)
$75~$100(17)
$100~$200(73)
$200~$300(53)
$300~$400(21)
$400~$500(27)
$500~$750(40)
$750~$1000(29)
$1000~$5000(99)
$5000~$10000(8)
$10000~$50000(1)
$50000~$100000(0)
$100000~$3000000(0)

Absorbance

0.02 AU max. at 330nm

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Bead type

Mag Sepharose Magnetic Beads

Bed Height

600 mm

30 mm

100 mm

25 mm

Bed Volume

320

5 mL

1 mL

Application

For the removal and/or purification of serine proteases e.g. thrombin

For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures

Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes

For purification of DNA from small molecules by gel filtration

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

Partial purification of an IgE-stimulating factor from a human T-cell line
Removal of BSA from an HIV-2 virus lysate
Purification of anti-mouse Fc IgG from sheep anti-serum

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

At least 10 mg histidine-tagged protein/ml sedimented medium.

Assay

=99%

99.8%

Average sample loading capacity

400 mg

50 mg

Bed Dimensions

16 x 100 mm

10 x 100 mm

6.4 x 100 mm

16 x 25 mm

26 x 600 mm

16 x 30 mm

7 x 25 mm

Autoignition Temp

~800 °F

800 °F

Application_4

Chromatography system|Manual

Cell isolation

Chromatography system

Assay Percent Range

99.8% min by GC

Applications

ion chromatography: suitable

Binding Capacity/ml Chromatography Medium

> 25 mg horse liver GST/mL resin

>10 mg/mL

>Approx. 10 mg GST-tagged protein/mL resin

At least 10 mg histidine-tagged protein

Capacity

1.35 meq/mL by wetted bed volume

0.7 meq/mL by wetted bed volume

1.0 meq/mL by wetted bed volume

1.3 meq/mL by wetted bed volume

1.8 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

=1.1 meq/mL by wetted bed volume

4.7 meq/g by dry weight

1.2 meq/mL by wetted bed volume

0.8 meq/mL by wetted bed volume

1.7 meq/mL by wetted bed volume

Avoid Using

Not stable below pH 2 nor to strong oxidising agents

BioProcess resin

yes

Boiling Point

189°C

2230 C

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

CAS_NO

69011-20-7

9004-54-0

11128-95-3

7631-86-9

126850-14-4

104219-63-8

9003-70-7

9041-35-4

9050-97-9

112945-52-5

60177-39-1

62610-50-8

65099-79-8

69011-15-0

39339-85-0

112926-00-8

1343-88-0

39389-20-3

63181-98-6

65546-95-4

1344-28-1

69011-19-4

Chem AlertStorageSymbol

Gray

ChEBI

CHEBI:28262

Chemical Stability (Column Hardware)

Stable in commonly used aqueous buffers, 1 M NaOH

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Chemical Substance ID

24852120

24854190

24863799

24865203

24852837

24854823

329759181

329759183

329759193

329768201

329759187

329768868

24857354

24867159

24854645

24854187

24854194

24857358

24857352

329756032

24854191

329752189

329763462

329752001

329763461

329759188

Application_5

Chromatography system|Manual

Chemical Stability

Commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature

Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl

Stable in all commonly used buffer systems

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile

Commonly used aqueous buffers

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Stable in aqueous buffers commonly used in Protein G chromatography, 6 M guanidine hydrochloride pH 4.7, 20 mM sodium phosphate + 1% SDS, 70% Ethanol, 95% Ethanol, 20 mM Sodium phosphate, 100 mM Glycine- phosphoric acid, 6 M Urea, 20% ethanol + 2% hibitan

Stable in most aqueous and organic eluent systems.

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Stable in all commonly used aqueous buffers

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 0.1 M acetic acid, 8 M urea, 6 M guanidine HCl, 24 % ethanol

Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea

Certificate of Analysis

Yes

Chem Alert Storage Symbol

Gray

CAS Registry Number 1

67-68-5

Chemical Name or Material

Dimethylsulfoxide

Column i.d.

26 mm

6.4

16 mm

Color

Colorless to slightly yellow

Gold/Brown

White

Chromatography technique

Affinity coupling

Antibody affinity chromatography

GST-tagged protein purification

Antibody affinity chromatography.

Group specific affinity

Histidine-tagged protein purification

Ion Exchange Chromatography

Hydrophobic Interaction Chromatography

Concentration

1 N in NaOH (Regenerant concentration)

Chemical/Physical Resistance

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Density Max.

1.2 g/ml

1.135 g/ml|

1.078 g/ml

Density

1.100g/cmÂ³

Column volume

1 mL

8 mL

COLOR

Blue

Composition

nitrogen content, =1.0%

Decomposition Information

>190°C

Color

Colorless

Flow Rate

2 ml/min - 10 ml/min

< 4 mL/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

< 10 ml/minThe pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.|< 10 ml/min å¡«å……åºŠä¸Šæ–¹çš

< 20 mL/min

< DarcyÂ´s Law

Recommended: 0.5 to 2.0 mL/min

< 4 mL/min |< 4 mL/min

< 5 ml/min|< 5 ml/min

0.8â€“3.9 mL/min

< 20 ml/min|< 20 ml/min

>60 ml/min|> 60 ml/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

< 5 mL/min

< 20 mL/min |< 20 mL/min

1-4ml/min

< 1 ml/min|< 1 ml/min

< 2.6 ml/min|< 2.6 ml/min

> 20 ml/min

> 4 ml/min

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

>10 ml/min

>60 ml/min

Flow Velocity

‰¤ 600 cm/h|‰¤ 600 cm/h

< 500 cm/h

30-150cm/h

< 150 cm/h

<15 cm/h

< 130 cm/h

< 600 cm/h|< 600 cm/h

â‰¤ 600 cm/h

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

< 130 cm/h|< 130 cm/h

< 300 cm/h

< 50 cm/h

< 60 cm/hour|< 60 cm/å°æ—¶

< 150 cm/h at 25Â°C, HR 16/10 columns, 5 cm bed height

< 300 cm/h, using XK 50/30 column with 15 cm bed height

<1800 cm/h

<1800 cm/h|< 1800 cm/h

15 cm/h|15 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

< 450 cm/h

< 450 cm/h|< 450 cm/h

10 cm/h - 50 cm/h|10 cm/h - 50 cm/h

Form

fine powder

fine crystals

beads

powder or crystals

Powder

Formula

O2Si

MgO₃Si

(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x

SiO₂

(C₁₀H₁₂ · C₁₀H₁₀ · C₈H₈ · C₃

Service & Support

Al2O3

Endotoxin Activity Max.

< 0.12 EU/ml

0.12 EU/mg

2 EU/ml

Flashpoint (°C)

> 240 C

Exclusion Limit Peptides

4 x 10Â³

Empirical Formula

MgO3Si

O2Si

Estimated Shelf Life from Manufacture Date

3 years

5 years

8 years

Feature

ferrule type packed column

Flash Point

87°C (188.6°F)

EC Number

231-545-4

215-681-1

215-691-6

238-878-4

Format

matrix material

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

Formula Weight

100.39

60.08

78.13

Format_6

Bulk resin

Prepacked columns

Prepacked column

Exclusion Limit

4 x 10³ (for Peptides) 5 x 10³ (small organics)

Grade

for column chromatography

high-purity grade

high-purity grade (Davisil Grade 62)

high-purity grade (Davisil Grade 633)

HPLC

high-purity grade (Davisil Grade 646)

Certified Reference Material

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

H-Codes

H372

H319

H373

H371

Grade

High-purity Grade (Davisil Grade 923)

High-purity Grade (7754)

High-purity Grade (Davisil Grade 643)

Standard

Technical Grade

High-purity Grade (Davisil Grade 636)

Nuclear Grade

Group To be Coupled

-NHâ‚‚

Primary amines

Identification

Pass Test

Health Hazard 2

WARNING!

InChIKey

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Includes

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Connectors for high- and low-pressure chromatography equipment and instructions.

Ion exchanger

Q (anion exchanger)

S (cation exchanger)

Ionic Capacity

0.15-0.20 mmol H⁺/mlÂ

0.18-0.25 mmol H⁺/ml medium

0.13-0.17 mmol/ml

0.18-0.24 mmol Cl^-/ml medium

0.11â€“0.16 mmol (Cl^-)/mL resin

3-4 mmol/g

0.07-0.09 mmol H⁺/ml medium

0.10â€“0.14 mmol Cl^- / mL resin

0.29-0.35 mmol Cl^-/ml medium

0.09-0.13 mmol H⁺/ml medium

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

Ligand

Native Protein A

Porcine heparin

Carboxymethyl

Cibacron Blue

Human IgG

Quaternary amine

Calmodulin

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Alkali-stabilized Protein A-derived (E. coli)

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Alkaline-stabilized protein A-derived (E.coli).

Bovine gelatin

Glutathione and 10-carbon linker arm

N-hydroxysuccinimide (NHS) activated

Phenyl

Sulphopropyl

Diethylaminoethyl

Ligand Coupling Method

Ether linkages

Epoxy activation

Ether linkages and hydrophilic spacer arm

Dextran surface extenders

Reductive Amination

Cyanogen bromide activation

Triazine

Epoxy

Maleimide activation

Ligand_7

Protein G

Protein L

Butyl, octyl, phenyl

Protein A

Protein A, alkali stabilized

Linear Formula

(C10H12 · C10H10 · C8H8 · C3H9N)X

MgO3Si

(CH₃)₂SO

(C10H12 · C10H10 · C8H8)x

SiO2

Intrinsic Viscosity

~ 17

IOD-Codes

Ion Exchanger Type

Strong anion exchanger

Weak anion

Multimodal weak cation exchanger

Strong cation exchanger

Weak anion exchanger

Weak cation exchanger

Weak and strong anion

MDL Number

MFCD00084428

MFCD00132722

MFCD00011232

MFCD00132705

MFCD00132770

MFCD00148280

MFCD00211881

MFCD00132707

MFCD00132771

MFCD00132718

MFCD00132726

MFCD00145831

MFCD03096474

MFCD00078249

MFCD00132701

MFCD00135579

MFCD00145842

MFCD00165824

MFCD00166483

MFCD00145586

MFCD03791384

MFCD00145841

MFCD00132712

Ligand

Recombinant Protein A (E.coli)

Ligand Density

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

3 mg GammaBind G, type 3/ml medium

Approx. 13 Î¼mol/ml

~ 7 Î¼mol Cibacron Blue/ml medium

2 mg Protein G/ml medium

~ 5 mg/mL resin

3 mg Protein A/ml medium

Ligand Concentration

~25 Âµmol phenyl/mL resin

~4mg heparin/mL resin

4.5-8 mg gelatin/ml drained resin

~45 Âµmol phenyl/mL resin

Matrix

cross-linked, 6% agarose particles

agarose, 6%, spherical

6% cross-linked agarose

Composite of cross-linked agarose

Highly cross-linked agarose with dextran surface extender

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

Rigid, cross-linked agarose, spherical

Cross-linked agarose, 4%

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

2% agarose

Cross-linked dextran

cross-linked agarose, 4%, spherical

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Composite of cross-linked agarose and dextran

Highly cross-linked 6% agarose

Matrix

Highly Cross-Linked Agarose

4% Agarose

4% Cross-Linked Agarose

Highly cross-linked agarose, spherical.

Highly cross-linked 4% agarose

Metal Ion Capacity

54 to 70 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

approx. 15 Âµmol Ni²⁺/ml medium

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

Molecular Weight

101.96

60.08

100.39

60.08

0.0

Material

Plastic/Stainless Steel

IUPAC Name

methylsulfinylmethane

Molecular Weight

78.129

Melting Point

18.4°C

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Material

N/A

Operating Flow Rate, Recommended

1.3 mL/min

0.75 mL/min

0.04 mL/min

0.075 mL/min

0.80 mL/min

0.50 mL/min

0.5 mL/min

0.30 mL/min

Optical Activity

6.5 (5% in slurry)

7.0 (5% in slurry)

~7 (5% in H 2 O, suspension)

Order Information

This product is part of our Custom Designed media program and not yet a standard product. If you are interested in large-scale quantities, please contact your local GE Healthcare representative. This product is produced based on a customer order. Delivery

HiLoad columns are prepacked XK columns.

For research use only.

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Tricorn high-performance columns have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Column not suitable for use with AKTAprime plus system.

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

Percoll PLUS is for research use only.

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

Operating Flow Rate, Max

1.2 mL/min, water at room temperature;
0.60 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.60 mL/min water at low temperature;
0.30 mL/min 20% ethanol or 10% glycerol at low temperature

1.80 mL/min, water at room temperature;
0.90 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.90 mL/min water at low temperature;
0.45 mL/min 20% ethanol or 10% glycerol at low temperature

0.15 mL/min, water at room temperature;
0.07 mL/min, 20% ethanol at room temperature;
0.07 mL/min water at low temperature;
0.03 mL/min 20% ethanol at low temperature

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

1.50 mL/min, water at room temperature;
0.75 mL/min, 20% ethanol at room temperature;
0.75 mL/min water at low temperature;
0.35 mL/min 20% ethanol at low temperature

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol at room temperature;
0.35 mL/min water at low temperature;
0.15 mL/min 20% ethanol at low temperature

< 4 mL/min

1.60 mL/min, water at room temperature;
0.80 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.80 mL/min water at low temperature;
0.40 mL/min 20% ethanol or 10% glycerol at low temperature

1.0 mL/min

2.6 mL/min

Osmolality

276-298 mOsm/kg

< 25 mOsm/kg

288-310 mOsm/kg

< 30 mOsm/kg

Pack size

15 g

1.5 g

5 Ã— 1 mL

6 x 1 L

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 columns

6 mL

1 Ã— 4.7 mL

10 g

2 x 1 mL

1 L

500 mL

1 Ã— 1 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

1.7 mL

100 mL

125 mL

25 g

2 x 1 ml 10% slurry

50 mL

100 g

5 mL

20 mL

300 mL

10 mL

200 mL

25 mL

500 g

6 x 500 mL

Packaging Layout

2.5, 5, 25 kg in fiber drum

10kg packaged in open-head fiber drums; 50g, 250g, 500 g, 1kg packaged in poly bottles

500 g in poly bottle

1 ft3 in fiber drum

25 kg in fiber drum

25, 500 g in poly bottle

1 kg in poly bottle

10, 50, 100, 500 g in poly bottle

500 g in poly drum

2.5 kg in poly drum

100, 500 g in poly bottle

5, 25 kg in fiber drum

P-CODES

P305 + P351 + P338

P260

P314

Partical Size Dry

>20 Âµm

50 Âµm-150 Âµm

>100 Âµm

20 Âµm-50 Âµm

40 Âµm-120 Âµm

18 Âµm-111 Âµm

Particle Size

24 Âµm-44 Âµm

25 Âµm-75 Âµm

30 Âµm-40 Âµm

60 Âµm

60 Î¼m-200 Î¼m

45 Âµm-165 Âµm

45 Âµm-165 Î¼m

45 Î¼m-165 Î¼m

24-44 Âµm

Particle size distribution, wet

100â€“300 Âµm

20â€“80 Âµm

55â€“165 Âµm

87â€“510 Âµm

15â€“88 Âµm

90â€“280 Âµm

Particle size, d_50V

~ 8.6 Âµm

~50 Âµm

~100 Î¼m

~34 Âµm

~34 Î¼m

~90 Î¼m

~75 Âµm

~ 9 Âµm

ImpRes = ~ 40 Î¼m, otherwise = ~ 75 Âµm

~90 Âµm

34 Âµm

~85 Âµm

pH Stability Cleaning-in-Place (CIP)

1 to 14

pH stability, CIP

2 (ligand dependent)â€“13 (ligand dependent)

2â€“9

4â€“9

2â€“12

2â€“13

2â€“13.7

3â€“12

3â€“10

4â€“13

3â€“13

2â€“10

3â€“14

1â€“14

2â€“14

pH stability, operational

2â€“9

4â€“13

4â€“9

3 to 12

3â€“11

3â€“13

3â€“10

2â€“12

2â€“10

pH 6 to 10

2â€“13

4â€“12

3â€“12

3â€“9

Pictogram

GHS08

GHS07

Pore Size

0.75 cm 3 /g pore volume

0.30 mL/g pore volume

0.8 cm 3 /g pore volume

60 Å average pore size (52-73 Å)

110 Å pore size

0.7-0.85 cm 3 /g pore volume (approx.)

~0.55 mL/g pore volume

~0.8 cm 3 /g pore volume

0.5 mL/g pore volume

0.7-0.9 cm 3 /g pore volume

30 Å

150 Å

90 Å mean pore size

60 Å mean pore size

Pressure/Flow Specification

24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d

Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.

>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height

min 90 cm/h, bed height 10 cm, column 5 cm i.d.

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

60-120 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.

base matrix 70-140 cm/h, pressure drop cm Hâ‚‚O/bed height=15 cm, bed height 10 cm, 5 cm i.d.

>11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 150 cm/h, bed height 10 cm, column 5 cm i.d.

>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

>480 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

pH ligand fully charged

Below 9

Above 6

< 9

Entire pH range

Physical Form

Liquid

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Molecular Formula

C2H6OS

Purity

>=99%

Pack Size

Packaging

Amber glass

Percent Purity

≥99.9%

pH Range

5.5â€“7.5

8.9â€“9.9

8.5â€“9.5

Properties

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Physical State

liquidSuspension

Solid

Liquid

Operating pH

0 - 7

0 - 14

Pressure/flow characteristics

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)

Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

S-Codes

26-36

Particle size distribution, dry

50 â€“ 150 Âµm

20 â€“ 80 Âµm

Particle Size in Methanol Max

163

Quality

monodispersed

dry

wet

regenerated

>1600 C

Quantity

100 g

500 g

50 g

SMILES

CS(=O)C

R-CODES

48/20

36/37/38

RTECS Number

VV7315000

VV7310000

Sample Volume

25 to 500 ÂµL

<= 13 mL

4 to 50 ÂµL

400 to 1000 Î¼l

0.5 to 1 ml (Gravity), 0.75 to 1.0 ml (Spin)

<= 5 ml

100 to 300 Î¼l

Resin Type

MabSelect

Sepharose CL-4B

Sepharose High Performance

Capto

Sepharose Fast Flow

Surface Area

289 m 2 /g

480 m 2 /g

500 m 2 /g (approx.)

800 m 2 /g

550 m 2 /g

=480 m 2 /g (approx.)

430-530 m 2 /g

250-350 m 2 /g

530 m 2 /g

380 m 2 /g

500 m 2 /g

175-225 m 2 /g

~500 m 2 /g

300 m 2 /g

470-530 m 2 /g

~30 m 2 /g

Storage

2 to 8Â°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, 20% Ethanol, 0.05 M Sodium Acetate

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

2ËšC to 8ËšC, 20% ethanol

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

2 to 8Â°C, 100% Isopropanol

2 to 8Â°C, 20% Ethanol

2-8C

2 to 8Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0

2Â°Câ€“8Â°C, 20% ethanol

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2 to 8Â°C

2 to 8ÂºC, 20% Ethanol, 50 mM Kâ‚‚HPOâ‚„, 1mM MgClâ‚‚ pH 7.2 in 20% Ethanol

4 to 30Â°C

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

Titratable Acid

0.001mEq/g max.

WGK

nwg

Osmolality

322-344 mOsm/kg

Pub Chem CID

679

Purity GradeNotes

Mobile phase for HPLC applications

Recommended Flow Rate

1 ml/min

5 ml/min

Sanitization Conditions

2% Hibitane/20% Ethanol or with 70% Ethanol

Scale

Lab|Process

Lab|Process development

Lab

Lab|Process development|Process

Particle Size in Methanol Min

Residueafter Evaporation

5ppm max.

Recommended maximum operational flow velocity

300 cm/h

Residue After Evaporation

5ppm max.

RIDADR

NONH for all modes of transport

Signal Word

Danger

Warning

Synonyms

Amberlite(R) IR120 hydrogen form

Silica gel 110 NH2

Silica, fumed; Silica; Silicic anhydride; Silicon dioxide; Silicon dioxide amorphous

Dowex(R) 1X8-200 Ion Exchange Resin

XAD16N

Activated magnesium silicate; Magnesium silicate

Silica gel

Stability

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

PurityGrade Notes

Mobile phase for HPLC applications

Refractive Index

1.4770 to 1.4800 (at 25°C)

Storage Conditions

4â€“30Â°C

4 - 8Â°C, 20% Ethanol, 10% medium slurry

4ËšC to 30ËšC, 20% ethanol

20% ethanol at 4Â°C to 30Â°C

Purity

99.8%

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Vapor Density

<1 (vs air)

>1 (vs air)

Viscosity Max

Stokes Radius

~ 10

Wetted Part

System

Sepax C-Pro|Sefia|Sepax 2

Recommended Cleaning-in-Place Reagent

2 M NaCl solution

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Stability

Stable

Synonym

DMSO

Swelling factor

~1.7

Theoretical Plates

> 43 000 m^-1

> 48 000 m^-1

> 13000 m-1

> 42 000 m^-1

5000 m-1

Vapor Pressure

17 mmHg ( 20 °C)

0.55mbar at 20Â°C

Well Volume

8.5

Typical Pressure Drop Over the Packed Bed

~ 3 MPa

~ 2 MPa

Type of ion exchanger

Strong anion exchanger (quaternary ammonium)

Strong cation exchanger (methyl sulfonate)

Viscosity

1.98 mPa/s at 25Â°C

Water

0.04% max.

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