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Temperature

45°C

Absorbance

0.02 AU max. at 330nm

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Additives

Without: Calcium, magnesium

Without: Calcium, magnesium, phenol red

Applications

gas chromatography (GC): suitable

LPLC: suitable

HPLC: suitable

solid phase extraction (SPE): suitable

Application

Purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®

Can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins

For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

For the removal and/or purification of serine proteases e.g. thrombin

Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag

Bed Volume

120

320

5 mL

1 mL

212 mL

Bed Dimensions

7 x 25 mm

16 x 100 mm

16 x 600 mm

16 x 25 mm

26 x 600 mm

10 x 100 mm

26 mm x 400 mm

6.4 x 100 mm

16 x 30 mm

3.2 x 300 mm

0.7 x 2.5 cm

1.6 x 2.5 cm

Application_4

Cell isolation

Chromatography system|Manual

Average sample loading capacity

400 mg

50 mg

Bed Height With Two Adaptors

0 to 200 mm

85 mm-350 mm

58 mm - 106 mm

0 mm-26 mm

158 mm-206 mm

680 mm-950 mm

Autoignition Temp

800 °F

~800 °F

Assay Percent Range

99.8% min by GC

Bed Height

100 mm

25 mm

600 mm

Bed Support

Net

Filter

Capacity

=25 % absorption capacity (water)

1.1 meq/mL by wetted bed volume

1.2 meq/mL by wetted bed volume

1.7 meq/mL by wetted bed volume

1.35 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

4.7 meq/g by dry weight

1.3 meq/mL by wetted bed volume

Application_5

Chromatography system|Manual

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

At least 10 mg histidine-tagged protein/ml sedimented medium.

Binding Capacity/ml Chromatography Medium

~ 13 mg ovalbumin

> 25 mg horse liver GST/mL resin

Up to 20 mg histidine-tagged protein

At least 10 mg histidine-tagged protein

Base Style

Flat Bottom

Insert Vial

CAS Registry Number 1

7631-86-9

67-68-5

CarbonLoad

15%

11%

20%

Autoclavable

Not Autoclavable

Brand

USA Scientific Brand

Axygen®

Bed Height With One Adaptor

0 mm-29 mm

129 mm-159 mm

185 mm-209 mm

825 mm-950 mm

CAS_NO

11128-95-3

126850-14-4

112926-00-8

39389-20-3

1343-88-0

9003-70-7

7631-86-9

9052-95-3

69011-20-7

104219-63-8

Avoid Using

Oxidizing agents, citrate buffers

Base Material

Anhydrous Na2SO4

Assay

=99%

Boiling Point

2230 C

189°C

Bore Size (mm)

ChEBI

CHEBI:28262

Certificate of Analysis

Yes

Chem Alert Storage Symbol

Gray

Chem AlertStorageSymbol

Gray

BioProcess resin

yes

Chemical Name or Material

Dimethylsulfoxide

Silica Gel

Chemical Stability

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons

Commonly used aqueous buffers

The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Chemical Substance ID

24870506

24852837

329750331

329759187

329763461

329759181

24857358

329763462

Certifications/Compliance

Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

Chromatography technique

Affinity coupling

Hydrophobic Interaction Chromatography

Group specific affinity

Ion Exchange Chromatography

Antibody affinity chromatography

GST-tagged protein purification

Histidine-tagged protein purification

Closure Material

Polyethylene

Aluminum

Plastic

Polypropylene

Color

Natural

Gold/Brown

N/A

Colorless to Slightly Yellow

Grey

Color

Red

Amber

Black

Clear

Brass

Colorless

Yellow

Gold

Silver

White

Column i.d.

10 mm

16 mm

26 mm

5 mm

50 mm

Column Type

capillary polar

capillary extremely polar

Mixed Mode

capillary chiral

capillary non-polar

packed GC

Reversed Phase

Compatibility

Compatible with most QuEChERS shakers.

Concentration

20 X

Content AndStorage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.

ClosureType

Screw Top

Screw

Snap Cap

Screw Cap

Density

1.100g/cmÂ³

Chemical/Physical Resistance

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Temp. Limits:. -10 C to 180 C isothermal and programmed

Closure Type

Crimp Cap

Screw Cap

With Titeseal Open-bottom Plug Style Plastic Closures, Unattached

Screw

Composition

Tris Base, Sodium Chloride

bed wt., 500 mg

magnesium sulfate, 150 mg

Discovery ® DSC-18, 400 mg

Closure Size

11 mm

9 mm

13 mm

20 mm

Density Max.

1.2 g/ml

ClosureSize

11 mm

9 mm

Column Format

Analytical Column

Analytical Column, EASY-Spray

Column volume

8 mL

1 mL

Closure Color

Black

Silver

ClosureMaterial

Polypropylene

COLOR

Blue

0.12 µm

0.20 µm

Diameter Metric Inner

6.6 mm

12 mm

15 mm

Diameter Metric Outer

15 mm

12 mm

8 mm

Diameter Metric Opening

11 mm

Decomposition Information

>190°C

Diameter Metric

75 µm

4.6 mm

2.1 mm

EC Number

231-545-4

262-373-8

200-838-9

231-153-3

215-609-9

215-681-1

238-878-4

Diameter MetricOpening

9 mm

Column Capacity

< 1 mg/mL

Feature

preconditioned

closure type black phenolic solid top cap

without needle Luer tip

Endcapped

syringe type Sample Lock

septum type Thermogreen ® LB-2

needle type N (cemented)

closure type screw top vial

Not Endcapped

Flow Direction

Supported bidirectionally. Certain applications may require orientation, i.e., vents. Reverse flow only for low pressure applications.

Flow Rate

< 20 ml/min|< 20 ml/min

1 ml/min - 4 ml/min

< 4 mL/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

4 ml/min (1 ml), 5 ml/min (5ml)|4 ml/min (1 ml), 5 ml/min (5ml)

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

< 31 ml/min

>10 ml/min

1.6 mL/min

2 ml/min - 10 ml/min

< 20 mL/min

< DarcyÂ´s Law

5 mL/min|5 mL/min

Recommended: 0.5 to 2.0 mL/min

Flow Velocity

< 300 cm/h

< 150 cm/h

â‰¤ 600 cm/h

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

<1800 cm/h

15 cm/h|15 cm/h

< 450 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

Exclusion Limit Peptides

4 x 10Â³

Form

liquid

fine crystals

powder

fine powder

powder or crystals

Format_6

Prepacked columns

Kits

Prepacked column

Bulk resin

Empirical Formula

MgO3Si

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

Flash Point

87°C (188.6°F)

Estimated Shelf Life from Manufacture Date

5 years

For Use With

Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100

FilmThickness

1 µm

Format

Liquid

Formula

Service & Support

MgO₃Si

(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x

[CH₂CH(C₆H₅)]_x[CH₂CH[C₆H₄

O2Si

Endotoxin Activity Max.

0.12 EU/mg

Format

matrix material

Formula Weight

100.39

60.08

78.13

Endcapped

Proprietary

Yes

Filtrate Vessel Material

Polycarbonate (PC)

Finish

Plain top

H-Codes

H315-H319-H335

H371

H317

H319

H351

H372

For Use With

ELSD, UV Detector

Height English

1.77 in.

1.25 in.

1.57 in.

Identification

Pass Test

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

Hold-up Volume of Membrane

<5 Î¼l

<10 Î¼l

<20 Î¼l

Housing

Polypropylene (PP)

Grade

HPLC

high-purity grade (Davisil Grade 923)

high-purity grade (7754)

technical grade

Standard

Certified Reference Material

Reagent Grade

Grade

High-purity Grade

High-purity Grade (Davisil Grade 633)

High-purity Grade (Davisil Grade 636)

Nuclear Grade

Graduated

Group To be Coupled

-NHâ‚‚

Primary amines

Guidance

For AKTAprime

Graduations

0.5, 1, 1.5 mL

Height Metric

43 mm

45 mm

32 mm

Health Hazard 2

WARNING!

Height English

1.69 in.

Includes

1 Ã— Column stand 4 Ã— PD-10 spin adaptor 1 Ã— Buffer tray 30 Ã— Bottom sleeve Instructions are supplied with the columns

Column valve V9-C
PEEK tubing i.d. 0.75 mm/ o.d. 1/16''
PEEK tubing i.d. 0.5 mm/o.d. 1/16''

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

Instructions supplied with the concentractors

Connectors for high- and low-pressure chromatography equipment and instructions.

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Height Metric

40 mm

Inlet id

2 mm (0.08 in)

1.55 mm (0.06 in)

0.75 mm (0.029 in)

1 mm (0.04 in)

1.0 mm (0.04 in)

Ion Exchanger Type

Multimodal weak cation exchanger

Strong cation exchanger

Weak cation exchanger

Weak anion exchanger

Strong anion exchanger

Weak anion

Ionic Capacity

0.13-0.17 mmol/ml

0.15-0.20 mmol H⁺/mlÂ

40-85 Âµmol/ml (Cl^-)

0.07-0.09 mmol H⁺/ml medium

0.10â€“0.14 mmol Cl^- / mL resin

0.18-0.25 mmol H⁺/ml medium

0.29-0.35 mmol Cl^-/ml medium

0.11â€“0.16 mmol (Cl^-)/mL resin

3-4 mmol/g

0.09-0.13 mmol H⁺/ml medium

0.18-0.24 mmol Cl^-/ml medium

Ion exchanger

Q (anion exchanger)

S (cation exchanger)

Inlet Connector

Stepped barb for 6 to 10 mm (1/4 to 3/8") tubing

Insert Type

Fused Insert

250µL Fused Insert

Pre-Slit

IOD-Codes

Length Metric

50 mm

400 mm

150 mm

100 mm

Ligand Coupling Method

Dextran surface extenders

Cyanogen bromide activation

Triazine

Epoxy

Maleimide activation

Ether linkages

Epoxy activation

Ligand Density

~ 7 Î¼mol Cibacron Blue/ml medium

2 mg Protein G/ml medium

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

6 mg rProtein A/ml medium

~ 5 mg/mL resin

3 mg GammaBind G, type 3/ml medium

Ligand_7

Protein A

Protein A, alkali stabilized

Protein A, alkali stablilized

2-mercaptopyridine

Protein L

Protein G

Length English

18 in.

Length Metric

20 mm

30 m

250 mm

Ligand

Porcine heparin

Recombinant protein A (E.coli)

Bovine gelatin

Glutathione and 10-carbon linker arm

N-hydroxysuccinimide (NHS) activated

Phenyl

Sulphopropyl

Carboxymethyl

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

TALONÂ® Superflow Cobalt-IMAC

Diethylaminoethyl

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Alkali-stabilized Protein A-derived (E. coli)

Cibacron Blue

Quaternary amine

Ligand Type

Monomeric C18

Kit Contents

RNAspin Lysis Solution (Red sticker): 125 ml, RNAspin Wash Buffer I (Yellow sticker): 80 ml, RNAspin Wash Buffer II (Grey sticker): 3 Ã— 25 ml Add 100 ml Ethanol to each bottle; RNAspin Desalting Buffer (Blue sticker); 125 ml, RNAspin Desalting Reaction B

Aprotinin (10 mg): 6500 M_r
Ribonuclease A (50 mg): 13 700 M_r
Carbonic anhydrase (15 mg): 29 000 M_r
Ovalbumin (50 mg): 43 000 M_r
Conalbumin (50 mg): 75 000 M_r
Blue Dextran 2000

Liner Material

Aluminum/Silicone

Polypropylene

PTFE/Silicone

Marking Spot

Yes

Material

aluminum liner

blunt tip Kel-F needle (point style 3)

clear glass bottle

polypropylene hardware

(Multi-Layer Foil: (1) 0.002" Polyethylene (2) 0.0003" Aluminum Foil (3) Polyethylene (4) Aluminum (metalized) (5) 60 Gauge Nylon)

black polypropylene cap

clear glass insert

glass TD tube

PE frit

Polycarbonate (PC)

SilTite

stainless steel frit

Borosilicate glass

Polypropylene

Polypropylene (PP)

PTFE frit

PTFE/red rubber septum

stainless steel TD tube

clear glass vial

Glutathione Sepharose 4 B

Mixed

PTFE body

PTFE/silicone septum

Tedlar ® PVDF film

N/A

Brass

polypropylene tube

Glass

Glass Body

PE frit (20 µm porosity)

Plastic/Stainless Steel

PTFE

bevel tip stainless steel needle (point style 2)

Silica Gel

Stainless Steel

Stainless steel hardware

VG2, 60% Vespel/40% Graphite

Material Septa

PTFE/Silicone

PTFE/Silicone/PTFE

Matrix

Rigid, cross-linked agarose, spherical

cross-linked, 6% agarose particles

Highly cross-linked agarose

4% Agarose

4% cross-linked agarose

cross-linked agarose, 4%, spherical

Composite of cross-linked agarose and dextran

Highly cross-linked 4% agarose

Highly cross-linked 6% agarose

Cross-linked dextran

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

6% cross-linked agarose

Composite of cross-linked agarose

Highly cross-linked agarose with dextran surface extender

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

Max Pressure

17400 psi (1200 bar)

5800 psi (400 bar)

4000 psi (275 bar)

11600 psi (800 bar)

4500 psi (310 bar)

MDL Number

MFCD00132718

MFCD00132726

MFCD00145831

MFCD03096474

MFCD00145586

MFCD03791384

MFCD00132712

MFCD00132722

MFCD00145841

MFCD00146442

MFCD00132707

MFCD00078249

MFCD00011232

Material

Borosilicate Glass, PTFE, Polyethylene, FKM/FPM

Fused Silica

Stainless Steel Column

Material Coating

Polypropylene

Material Plunger

Stainless Steel

Max Temperature Metric

50°C

Metal Ion Capacity

~15 Âµmol Ni²⁺/ml medium

54 to 70 Âµmol Ni²⁺/ml medium

approx. 15 Âµmol Ni²⁺/ml medium

InChIKey

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Includes

Two (0.5 m and 2 m L) 1/16 in. OD PTFE tubing assembly with 1/4-28 UNF PP fitting at one end, a frit kit (contains two each of 10 µm PE, 10 µm PTFE, and 30 µm PTFE frits), and a Click-N-Seal 10-32 PEEK conical port fitting.

Vials and Closures

3.5 in. Coil

Model

725

Operating Flow Rate, Max

1.0 mL/min

2.6 mL/min

0.15 mL/min, water at room temperature;
0.07 mL/min, 20% ethanol at room temperature;
0.07 mL/min water at low temperature;
0.03 mL/min 20% ethanol at low temperature

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

Operating pH

0 - 14

1 - 8

2 - 8

3.8 - 6.8

1 - 9

2 - 9

2 - 7.5

Order code

SH30237.01

SH30031.02

SH30268.01

SH30588.01

Osmolality

322-344 mOsm/kg

< 25 mOsm/kg

288-310 mOsm/kg

Outlet Connector

Stepped barb for 6 to 10 mm (1/4 to 3/8") tubing

Outlet id

0.75 mm (0.029 in)

1.55 mm (0.06 in)

1.0 mm (0.04 in)

p H

2.5 to 8

2.5 to 7.5

8.5 pH

2 to 8

4 to 13

7.5 pH

2.5 to 7

Pack Size

Pack size

100 g

20 columns

30 columns

5 mL

10 mL

200 mL

25 mL

4 x 50 mL

4 x 53 mL

500 g

6 x 500 mL

1 Ã— 320 mL

1 Ã— 4.7 mL

10 g

2 x 1 mL

5 Ã— 1 mL

1 piece

20 mL

300 mL

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 Ã— 100 ÂµL

50 columns

6 mL

7 Ã— 1 mL

1000 mL

100 mL

50 mL

1 L

1 Ã— 1 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

500 mL

ParticleSize

Monolith

2.2 µm

5 µm

3 µm

pH stability, CIP

2â€“10

3â€“14

1â€“14

2â€“14

2â€“10.5

3â€“10

4â€“13

2â€“14Co²⁺-stripped medium

2â€“9

2â€“13

2â€“13.7

3â€“12

pH stability, operational

2â€“13

4â€“12

3 to 12

3â€“13

3â€“12

3â€“9

3â€“10

2â€“9

4â€“13

4â€“9

2â€“12

2â€“10

pH 6 to 10

Pore Size

160 Å

~1.30 mL/g pore volume

60 +?

60 Å mean pore size

70 Å pore size

~0.55 mL/g pore volume

0.45

120 Å

90 Å

1000 Å

100 Å

150 Å

90 Å mean pore size

0.8 cm 3 /g pore volume

60 Å average pore size (52-73 Å)

0.75 cm 3 /g pore volume

180 Å

0.7-0.9 cm 3 /g pore volume

30 Å

300 Å

90 Å pore size

110 Å pore diameter

60 Å pore size

100 Å

0.7-0.85 cm 3 /g pore volume (approx.)

PoreSize

130 Å (Mesopore Size), 2 µm (Macropore Size)

80 Å

100 Å

300 Å

Power Consumption

8 VA

Preslit

Yes

Pressure/Flow Specification

>480 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

>11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

Product Line

20 mm Aluminum Crimp Seal

Pierce, Tween

Pierce

Easy Spray

Length English Needle

2 in.

Ligand Concentration

4.5-8 mg gelatin/ml drained resin

~45 Âµmol phenyl/mL resin

Linear Formula

SiO2

(C10H12 · C10H10 · C8H8)x

MgO3Si

(CH₃)₂SO

Molecular Weight

0.0

84.93

78.129

100.39

60.08

No per Pack

100

No per Pack

1000

100

Order Information

HiLoad columns are prepacked XK columns

HiLoad columns are prepacked XK columns.

Not suitable for use with AKTAprime plus system.

This product is produced based on a customer order. Delivery time may be longer than for standard products.

Percoll PLUS is for research use only.

For research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

Packaging Layout

100, 500 g in poly bottle

5, 25 kg in fiber drum

1 ft3 in fiber drum

25 kg in fiber drum

25, 500 g in poly bottle

1, 6x1, 2.5 kg in poly bottle

2.5, 5, 25 kg in fiber drum

1 kg in poly bottle

Packing Material

Spherical, Solid Core, Ultrapure Silica

Type B Silica

Spherical, Fully Porous, Base-Deactivated Silica

Spherical, Nanopolymer Hybrid Silica

Spherical, Fully Porous, Ultrapure Silica

Particle Shape

Spherical

Particle size distribution, wet

90â€“280 Âµm

Particle Size in Methanol Min

Particle size, d_50V

~85 Âµm

~75 Âµm

~ 8.6 Âµm

~90 Âµm

34 Âµm

~34 Âµm

~34 Î¼m

~ 9 Âµm

~90 Î¼m

pH Stability Working Range

3â€“12

Porosity

20 Î¼m

10 or 80 Âµm

10 Î¼m

7 Î¼m

Pressure Limit

2 MPa

20 bar (2 MPa, 290 psi)

Properties

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Purity

99.8%

>=99%

Qty/CS

400 / Cs

Quantity

100

50 g

10 g

100 Pack

500 mL

100 g

144 Pack

1000/Cs.

200

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

S-Codes

26-36

53.1-26-45

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Sample Volume

600 Âµl

< 150 Âµl

<= 1.5 ml

25 to 500 ÂµL

<= 13 ml

4 to 50 ÂµL

<= 15 ml

<= 5 ml

< 0.5 mL

< 5 mL

Shipping Dimensions - L in

Signal Word

Warning

Danger

Surface Area

~30 m 2 /g

170 m 2 /g

300 m²/g

289 m 2 /g

450 m 2 /g

480 m 2 /g

250-350 m 2 /g

530 m 2 /g

22 m 2 /g

320 m 2 /g

450 m 2 /g

300 m 2 /g

470-530 m 2 /g

500 m 2 /g (approx.)

800 m 2 /g

~500 m 2 /g

100 m 2 /g

500 m 2 /g

=480 m 2 /g (approx.)

200 m 2 /g

430-530 m 2 /g

475 m 2 /g

80 m 2 /g

450 m²/g

100 m²/g

Swelling factor

~1.7

Synonyms

Crimp Seal with Red Rubber/PTFE Septum

Dowex(R) 1X8-200 Ion Exchange Resin

QuEChERS; QuEChERS

Tedlar(R) Gas Sampling Bag

XAD16N

Activated magnesium silicate; Magnesium silicate

QuEChERS

Silica gel

Operating Flow Rate, Recommended

0.75 mL/min

0.075 mL/min

0.80 mL/min

1.3 mL/min

0.5 mL/min

Partical Size Dry

>100 Âµm

50 Âµm-150 Âµm

18 Âµm-111 Âµm

40 Âµm-120 Âµm

Particle Size

60 Î¼m-200 Î¼m

60 Î¼m-160 Î¼m

2 µm

24 Âµm-44 Âµm

60 Âµm-165 Âµm

24-44 Âµm

45 Âµm-165 Âµm

45 Î¼m-165 Î¼m

pH ligand fully charged

< 9

Entire pH range

Below 9

Above 6

Physical State

solid

liquidSuspension

Liquid

Molecular Formula

O2Si

C2H6OS

Pressure/flow characteristics

Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

IUPAC Name

methylsulfinylmethane

PurityGrade Notes

Mobile phase for HPLC applications

Quality

regenerated

dry

monodispersed

with moisture indicator free of heavy metals

Theoretical Plates

5000 m-1

> 38 000 m^-1

> 43 000 m^-1

> 13000 m-1

> 48 000 m^-1

ThicknessMetric

1.3 mm

Quantity

100/Pk.

1000/Pk.

1000

Each

R-CODES

36/37/38

48/20

Relative Humidity

20%-95% (non condensing)

Thread Size

13-425

9 mm

10-425

20 mm

Scale

Lab|Process development

Lab

Lab|Process development|Process

Lab|Process

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Stationary Phase

Reversed Phase/Anion Exchange/Cation Exchange

C18

Storage Conditions

4 - 30Â°C

At room temperature. Precipitation can occur at low temperature.

4ËšC to 30ËšC, 20% ethanol

4â€“30Â°C

Operating Pressure Max

4.1 bar (60 psi)

Resin Type

Sepharose High Performance

MabSelect

Capto

Sepharose Fast Flow

P-CODES

P305 + P351 + P338

P280

P314

Physical Form

Liquid

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Volume Metric

2 mL

2.0 mL

Water

0.04% max.

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

ProductLine

Acclaim

>1600 C

Stability

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Storage

15Â° to 30Â° C

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol

2Â°Câ€“8Â°C, 20% ethanol

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

4 to 30Â°C

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

2-8C

4Â°C to 8Â°C

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

MarkingSpot

Yes

Matrix

Highly crossed-linked agarose, 6%

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Needle Point Style

Blunt

Noper Pack

100

RTECS Number

VV7315000

Packaging

Amber glass

Intrinsic Viscosity

~ 17

Percent Purity

≥99.9%

7.0 to 7.4

pH Range

5.5â€“7.5

8.5â€“9.5

8.9â€“9.9

Optical Activity

6.5 (5% in slurry)

~7 (5% in H 2 O, suspension)

7.0 (5% in slurry)

Total binding capacity

~50 mg hIgG/mL resin

pH Stability Cleaning-in-Place (CIP)

1 to 14

Pictogram

GHS08

GHS07

VialType

11 mm Crimp Top Vial

9 mm Target DP Vial

Volume

400 Âµl - 2 ml

22 mL

3 mL

50 mL

6 mL

2 mL

25 mL

100-500 Âµl

1 mL

0.5 mL

2-6 ml

5-20 ml

System

Sepax C-Pro|Sefia|Sepax 2

Sealing

Heat-fused

Septum

None

Melting Point

18.4°C

Recommended maximum operational flow velocity

300 cm/h

Residue After Evaporation

5ppm max.

RIDADR

NONH for all modes of transport

Sterilization

0.1 Âµm sterile filtered

May be autoclaved at 121Â°C for 20 minutes. Multiple autoclave cycles are possible. However, the responsibility for reuse is with the operator. The device should be protected from cross contamination. An integrity test should be performed after autoclavin

Titratable Acid

0.001mEq/g max.

SMILES

CS(=O)C

Volume MetricUsable

4 mL

1.5 mL

Volume_28

5-20 ml

400 Âµl - 2 ml

2-6 ml

100-500 Âµl

WGK

nwg

Refractive Index

1.4770 to 1.4800 (at 25°C)

Osmolality

< 30 mOsm/kg

Phase

Reversed Phase and Ion Exchange

Reversed Phase

Particle Size in Methanol Max

163

Pub Chem CID

679

Septum

Red PTFE/White Silicone

Purity GradeNotes

Mobile phase for HPLC applications

Type

Analytical Column

Liquid Chromatography (LC)

HPLC Column

Recommended Flow Rate

1 ml/min

USP Type

Viscosity

1.98 mPa/s at 25Â°C

Volume Metric

4 mL

Volume Metric Total

2 mL

Temperature

60°C

Solubility Information

Solubility aq. soln.: insoluble.

Residueafter Evaporation

5ppm max.

Shelf Life

24 months

Total Time of Preparation

< 30 min/6 preps

U SPType

Vapor Pressure

0.55mbar at 20Â°C

17 mmHg ( 20 °C)

Wetted Part

yes

Typical Pressure Drop Over the Packed Bed

~ 2 MPa

~ 3 MPa

Volume Metric Residual

<170µL

Support System

Polypropylene (PP)

Stokes Radius

~ 10

Suitability

Autosampler compatibility:Agilent Agilent 1050/1090A Agilent 1100/1200 Agilent 7673A, 7683 Agilent 5890/7985A/6890Hitachi AS-1000, AS-2000, AS-4000 L7200/L7250LEAP Technologies CTC LC PAL, GC PAL CombiPAL HTC PAL/HTS PALPerkinElmer Autosystem/AS-2000/XL

Vapor Density

<1 (vs air)

Viscosity Max

Volume Metric Usable

1.5 mL

Synonym

DMSO

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Strong anion exchanger (quaternary ammonium)

Vent

On inlet

Volume MetricTotal

2 mL

Volume MetricResidual

<170µL

Volume English

1 dr.

Volume Metric Working

1.8 mL

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