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Chromatography

Chromatography Consumables

Reagents and Buffers

Chromatography Media

$0~$10(0)
$10~$25(0)
$25~$50(182)
$50~$75(52)
$75~$100(17)
$100~$200(73)
$200~$300(53)
$300~$400(21)
$400~$500(27)
$500~$750(40)
$750~$1000(29)
$1000~$5000(99)
$5000~$10000(8)
$10000~$50000(1)
$50000~$100000(0)
$100000~$3000000(0)

Absorbance

0.02 AU max. at 330nm

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Application

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

Partial purification of an IgE-stimulating factor from a human T-cell line
Removal of BSA from an HIV-2 virus lysate
Purification of anti-mouse Fc IgG from sheep anti-serum

For purification of DNA from small molecules by gel filtration

Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

For the removal and/or purification of serine proteases e.g. thrombin

Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag

Application_4

Chromatography system

Chromatography system|Manual

Cell isolation

Autoignition Temp

800 °F

~800 °F

Bed Volume

5 mL

320

1 mL

Application_5

Chromatography system|Manual

Applications

ion chromatography: suitable

Bed Dimensions

16 x 30 mm

10 x 100 mm

6.4 x 100 mm

16 x 100 mm

7 x 25 mm

16 x 25 mm

26 x 600 mm

Assay

99.8%

=99%

Average sample loading capacity

400 mg

50 mg

Avoid Using

Not stable below pH 2 nor to strong oxidising agents

Assay Percent Range

99.8% min by GC

Bed Height

100 mm

30 mm

25 mm

600 mm

Binding Capacity/ml Chromatography Medium

At least 10 mg histidine-tagged protein

> 25 mg horse liver GST/mL resin

>10 mg/mL

>Approx. 10 mg GST-tagged protein/mL resin

Binding Capacity/Column

At least 10 mg histidine-tagged protein/ml sedimented medium.

Approx. 40 mg histidine-tagged protein/ml medium

Capacity

0.8 meq/mL by wetted bed volume

0.7 meq/mL by wetted bed volume

1.0 meq/mL by wetted bed volume

4.7 meq/g by dry weight

1.7 meq/mL by wetted bed volume

1.8 meq/mL by wetted bed volume

1.35 meq/mL by wetted bed volume

1.2 meq/mL by wetted bed volume

=1.1 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

1.3 meq/mL by wetted bed volume

Bead type

Mag Sepharose Magnetic Beads

Boiling Point

2230 C

189°C

CAS Registry Number 1

67-68-5

CAS_NO

112945-52-5

1344-28-1

11128-95-3

104219-63-8

126850-14-4

39339-85-0

112926-00-8

1343-88-0

9003-70-7

9041-35-4

9050-97-9

39389-20-3

60177-39-1

62610-50-8

69011-19-4

65099-79-8

69011-15-0

63181-98-6

65546-95-4

7631-86-9

9004-54-0

69011-20-7

ChEBI

CHEBI:28262

Certificate of Analysis

Yes

Chem AlertStorageSymbol

Gray

Chem Alert Storage Symbol

Gray

Chemical Name or Material

Dimethylsulfoxide

Chemical Stability

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 0.1 M acetic acid, 8 M urea, 6 M guanidine HCl, 24 % ethanol

Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea

Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl

Stable in all commonly used buffer systems

Stable in all commonly used aqueous buffers

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile

Stable in aqueous buffers commonly used in Protein G chromatography, 6 M guanidine hydrochloride pH 4.7, 20 mM sodium phosphate + 1% SDS, 70% Ethanol, 95% Ethanol, 20 mM Sodium phosphate, 100 mM Glycine- phosphoric acid, 6 M Urea, 20% ethanol + 2% hibitan

Stable in most aqueous and organic eluent systems.

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Commonly used aqueous buffers

Commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature

Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Stable in commonly used aqueous buffers, 1 M NaOH

Chemical Substance ID

24854187

24854191

24854645

24857358

24854194

24857352

329756032

24863799

24865203

329759188

24852120

24854190

329752001

329752189

329763462

24852837

24854823

329763461

329759181

329759187

329768868

329759183

329759193

329768201

24857354

24867159

Chromatography technique

Affinity coupling

Hydrophobic Interaction Chromatography

Antibody affinity chromatography

GST-tagged protein purification

Ion Exchange Chromatography

Antibody affinity chromatography.

Group specific affinity

Histidine-tagged protein purification

COLOR

Blue

Chemical/Physical Resistance

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Color

Gold/Brown

Colorless to Slightly Yellow

Column i.d.

26 mm

16 mm

6.4

Color

Colorless

White

Column volume

8 mL

1 mL

Composition

nitrogen content, =1.0%

Density Max.

1.078 g/ml

1.135 g/ml|

1.2 g/ml

Density

1.100g/cmÂ³

Decomposition Information

>190°C

Concentration

1 N in NaOH (Regenerant concentration)

EC Number

215-691-6

231-545-4

215-681-1

238-878-4

Flow Rate

2 ml/min - 10 ml/min

< 4 ml/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

< 20 ml/min

< DarcyÂ´s Law

Recommended: 0.5 to 2.0 mL/min

< 1 ml/min|< 1 ml/min

< 2.6 ml/min|< 2.6 ml/min

> 20 mL/min

> 4 ml/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

< 5 ml/min

< 20 mL/min |< 20 mL/min

1-4ml/min

< 20 ml/min|< 20 ml/min

>60 ml/min|> 60 ml/min

< 10 ml/minThe pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.|< 10 ml/min å¡«å……åºŠä¸Šæ–¹çš

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

>10 ml/min

>60 ml/min

< 4 mL/min |< 4 mL/min

< 5 ml/min|< 5 ml/min

0.8â€“3.9 mL/min

Flow Velocity

‰¤ 600 cm/h|‰¤ 600 cm/h

< 500 cm/h

30-150cm/h

< 130 cm/h

< 600 cm/h|< 600 cm/h

â‰¤ 600 cm/h

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

< 450 cm/h

< 450 cm/h|< 450 cm/h

< 150 cm/h at 25Â°C, HR 16/10 columns, 5 cm bed height

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

< 300 cm/h, using XK 50/30 column with 15 cm bed height

<1800 cm/h

<1800 cm/h|< 1800 cm/h

15 cm/h|15 cm/h

< 130 cm/h|< 130 cm/h

< 300 cm/h

< 50 cm/h

< 60 cm/hour|< 60 cm/å°æ—¶

< 150 cm/h

<15 cm/h

10 cm/h - 50 cm/h|10 cm/h - 50 cm/h

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

Endotoxin Activity Max.

< 0.12 EU/ml

0.12 EU/mg

2 EU/ml

Empirical Formula

MgO3Si

O2Si

Estimated Shelf Life from Manufacture Date

3 years

8 years

5 years

Feature

ferrule type packed column

Flashpoint (°C)

> 240 C

Flash Point

87°C (188.6°F)

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

Form

fine powder

fine crystals

powder

powder or crystals

beads

Exclusion Limit Peptides

4 x 10Â³

Format

matrix material

Formula

(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x

O2Si

MgO₃Si

(C₁₀H₁₂ · C₁₀H₁₀ · C₈H₈ · C₃

SiO₂

Al2O3

Service & Support

Format_6

Bulk resin

Prepacked columns

Prepacked column

Formula Weight

100.39

60.08

Formula Weight

78.13

Exclusion Limit

4 x 10³ (for Peptides) 5 x 10³ (small organics)

Grade

high-purity grade (Davisil Grade 923)

high-purity grade (7754)

technical grade

for column chromatography

high-purity grade

high-purity grade (Davisil Grade 62)

Standard

high-purity grade (Davisil Grade 646)

certified reference material

high-purity grade (Davisil Grade 636)

nuclear grade

HPLC

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

H-Codes

H371

H372

H319

H373

Group To be Coupled

-NHâ‚‚

Primary amines

Grade

High-purity Grade (Davisil Grade 633)

High-purity Grade (Davisil Grade 643)

Health Hazard 2

WARNING!

Identification

Pass Test

Includes

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Connectors for high- and low-pressure chromatography equipment and instructions.

Ion exchanger

Q (anion exchanger)

S (cation exchanger)

Ionic Capacity

0.15-0.20 mmol H⁺/mlÂ

0.13-0.17 mmol/ml

0.07-0.09 mmol H⁺/ml medium

0.10â€“0.14 mmol Cl^- / mL resin

0.18-0.24 mmol Cl^-/ml medium

0.09-0.13 mmol H⁺/ml medium

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

0.18-0.25 mmol H⁺/ml medium

0.11â€“0.16 mmol (Cl^-)/mL resin

3-4 mmol/g

0.29-0.35 mmol Cl^-/ml medium

Ion Exchanger Type

Multimodal weak cation exchanger

Strong cation exchanger

Strong anion exchanger

Weak anion

Weak anion exchanger

Weak and strong anion

Weak cation exchanger

IOD-Codes

Intrinsic Viscosity

~ 17

Ligand

Porcine heparin

Recombinant protein A (E.coli)

Bovine gelatin

Glutathione and 10-carbon linker arm

N-hydroxysuccinimide (NHS) activated

Phenyl

Alkaline-stabilized protein A-derived (E.coli).

Alkali-stabilized Protein A-derived (E. coli)

Calmodulin

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Native protein A

Cibacron Blue

Human IgG

Quaternary amine

Diethylaminoethyl

Carboxymethyl

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Sulphopropyl

Ligand Density

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

~ 5 mg/mL resin

Approx. 13 Î¼mol/ml

~ 7 Î¼mol Cibacron Blue/ml medium

2 mg Protein G/ml medium

3 mg GammaBind G, type 3/ml medium

3 mg Protein A/ml medium

InChIKey

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Ligand Coupling Method

Cyanogen bromide activation

Triazine

Dextran surface extenders

Ether linkages and hydrophilic spacer arm

Ether linkages

Epoxy activation

Reductive Amination

Epoxy

Maleimide activation

Matrix

cross-linked, 6% agarose particles

Highly cross-linked agarose

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

4% Agarose

4% cross-linked agarose

cross-linked agarose, 4%, spherical

Cross-linked agarose, 4%

6% cross-linked agarose

Composite of cross-linked agarose

Highly cross-linked agarose with dextran surface extender

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Highly cross-linked agarose, spherical.

agarose, 6%, spherical

2% agarose

Cross-linked dextran

Composite of cross-linked agarose and dextran

Highly cross-linked 6% agarose

Matrix

Highly crossed-linked agarose, 6%

Rigid, cross-linked agarose, spherical

Highly cross-linked 4% agarose

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Linear Formula

(CH₃)₂SO

MgO3Si

(C10H12 · C10H10 · C8H8 · C3H9N)X

(C10H12 · C10H10 · C8H8)x

SiO2

Ligand_7

Protein A

Protein A, alkali stabilized

Protein G

Butyl, octyl, phenyl

Protein L

MDL Number

MFCD00145842

MFCD00165824

MFCD00166483

MFCD00145586

MFCD03791384

MFCD00078249

MFCD00132701

MFCD00135579

MFCD00132718

MFCD00132726

MFCD00145831

MFCD03096474

MFCD00132707

MFCD00132771

MFCD00011232

MFCD00084428

MFCD00132705

MFCD00132770

MFCD00148280

MFCD00211881

MFCD00132722

MFCD00145841

MFCD00132712

Licensing

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

Material

Plastic/Stainless Steel

N/A

BioProcess resin

yes

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

Ligand Concentration

~45 Âµmol phenyl/mL resin

~25 Âµmol phenyl/mL resin

~4mg heparin/mL resin

4.5-8 mg gelatin/ml drained resin

Metal Ion Capacity

54 to 70 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

approx. 15 Âµmol Ni²⁺/ml medium

Molecular Formula

C2H6OS

Molecular Weight

60.08

101.96

60.08

78.129

100.39

0.0

Melting Point

18.4°C

Operating Flow Rate, Max

< 4 mL/min

1.60 mL/min, water at room temperature;
0.80 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.80 mL/min water at low temperature;
0.40 mL/min 20% ethanol or 10% glycerol at low temperature

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

1.0 mL/min

2.6 mL/min

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol at room temperature;
0.35 mL/min water at low temperature;
0.15 mL/min 20% ethanol at low temperature

0.15 mL/min, water at room temperature;
0.07 mL/min, 20% ethanol at room temperature;
0.07 mL/min water at low temperature;
0.03 mL/min 20% ethanol at low temperature

1.2 mL/min, water at room temperature;
0.60 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.60 mL/min water at low temperature;
0.30 mL/min 20% ethanol or 10% glycerol at low temperature

1.80 mL/min, water at room temperature;
0.90 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.90 mL/min water at low temperature;
0.45 mL/min 20% ethanol or 10% glycerol at low temperature

1.50 mL/min, water at room temperature;
0.75 mL/min, 20% ethanol at room temperature;
0.75 mL/min water at low temperature;
0.35 mL/min 20% ethanol at low temperature

Optical Activity

~7 (5% in H 2 O, suspension)

7.0 (5% in slurry)

6.5 (5% in slurry)

Order Information

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

For research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

Percoll PLUS is for research use only.

HiLoad columns are prepacked XK columns.

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Tricorn high-performance columns have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Column not suitable for use with AKTAprime plus system.

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

This product is part of our Custom Designed media program and not yet a standard product. If you are interested in large-scale quantities, please contact your local GE Healthcare representative. This product is produced based on a customer order. Delivery

Pack size

2 x 1 ml 10% slurry

50 mL

1 L

100 g

5 mL

1 Ã— 1 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

1.7 mL

100 mL

125 mL

25 g

1 Ã— 4.7 mL

10 g

2 x 1 mL

1.5 g

5 Ã— 1 mL

6 x 1 L

500 mL

15 g

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 columns

6 mL

20 mL

300 mL

10 mL

200 mL

25 mL

500 g

6 x 500 mL

Partical Size Dry

18 Âµm-111 Âµm

20 Âµm-50 Âµm

40 Âµm-120 Âµm

>100 Âµm

50 Âµm-150 Âµm

>20 Âµm

Particle size, d_50V

~90 Âµm

34 Âµm

~100 Î¼m

~34 Âµm

~34 Î¼m

~ 9 Âµm

ImpRes = ~ 40 Î¼m, otherwise = ~ 75 Âµm

~75 Âµm

~ 8.6 Âµm

~50 Âµm

~90 Î¼m

~85 Âµm

pH stability, operational

2â€“10

pH 6 to 10

2â€“13

4â€“12

2â€“12

3 to 12

3â€“11

3â€“13

2â€“9

4â€“13

4â€“9

3â€“10

3â€“12

3â€“9

Physical State

Liquid

solid

liquidSuspension

Operating Flow Rate, Recommended

0.50 mL/min

0.75 mL/min

0.5 mL/min

1.3 mL/min

0.04 mL/min

0.075 mL/min

0.80 mL/min

0.30 mL/min

Operating pH

0 - 7

0 - 14

Osmolality

< 25 mOsm/kg

322-344 mOsm/kg

288-310 mOsm/kg

Packaging Layout

1 kg in poly bottle

10, 50, 100, 500 g in poly bottle

2.5 kg in poly drum

1 ft3 in fiber drum

25 kg in fiber drum

25, 500 g in poly bottle

500 g in poly drum

2.5, 5, 25 kg in fiber drum

10kg packaged in open-head fiber drums; 50g, 250g, 500 g, 1kg packaged in poly bottles

500 g in poly bottle

100, 500 g in poly bottle

5, 25 kg in fiber drum

pH ligand fully charged

Above 6

< 9

Entire pH range

Below 9

Pictogram

GHS07

GHS08

Pore Size

110 Å pore size

~0.8 cm 3 /g pore volume

0.5 mL/g pore volume

0.7-0.9 cm 3 /g pore volume

30 Å

~0.55 mL/g pore volume

0.7-0.85 cm 3 /g pore volume (approx.)

0.75 cm 3 /g pore volume

0.30 mL/g pore volume

0.8 cm 3 /g pore volume

60 Å average pore size (52-73 Å)

150 Å

90 Å mean pore size

60 Å mean pore size

Pressure/Flow Specification

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

>480 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 150 cm/h, bed height 10 cm, column 5 cm i.d.

>11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height

>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

60-120 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.

base matrix 70-140 cm/h, pressure drop cm Hâ‚‚O/bed height=15 cm, bed height 10 cm, 5 cm i.d.

24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d

Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.

250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height

min 90 cm/h, bed height 10 cm, column 5 cm i.d.

>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

Osmolality

< 30 mOsm/kg

276-298 mOsm/kg

Particle Size

45 Âµm-165 Âµm

45 Âµm-165 Î¼m

45 Î¼m-165 Î¼m

24-44 Âµm

60 Î¼m-200 Î¼m

60 Âµm

24 Âµm-44 Âµm

25 Âµm-75 Âµm

30 Âµm-40 Âµm

Particle size distribution, dry

20 â€“ 80 Âµm

50 â€“ 150 Âµm

Particle size distribution, wet

55â€“165 Âµm

87â€“510 Âµm

15â€“88 Âµm

100â€“300 Âµm

20â€“80 Âµm

90â€“280 Âµm

pH stability, CIP

2â€“10

3â€“14

3â€“13

3â€“10

4â€“13

2â€“12

2â€“13

2â€“13.7

3â€“12

2 (ligand dependent)â€“13 (ligand dependent)

2â€“9

4â€“9

1â€“14

2â€“14

pH Range

8.9â€“9.9

8.5â€“9.5

5.5â€“7.5

Quality

wet

regenerated

dry

monodispersed

Recommended Flow Rate

5 ml/min

1 ml/min

Refractive Index

1.4770 to 1.4800 (at 25°C)

Pressure/flow characteristics

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Sample Volume

0.5 to 1 ml (Gravity), 0.75 to 1.0 ml (Spin)

25 to 500 ÂµL

400 to 1000 Î¼l

<= 13 ml

4 to 50 ÂµL

<= 5 ml

100 to 300 Î¼l

P-CODES

P305 + P351 + P338

P260

P314

PurityGrade Notes

Mobile phase for HPLC applications

Pub Chem CID

679

Physical Form

Liquid

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Particle Size in Methanol Max

163

Properties

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Quantity

500 g

100 g

50 g

pH Stability Cleaning-in-Place (CIP)

1 to 14

Resin Type

Sepharose High Performance

Capto

Sepharose Fast Flow

MabSelect

Sepharose CL-4B

Recommended maximum operational flow velocity

300 cm/h

Purity GradeNotes

Mobile phase for HPLC applications

Residue After Evaporation

5ppm max.

RIDADR

NONH for all modes of transport

Storage

2 to 8Â°C

2 to 8ÂºC, 20% Ethanol, 50 mM Kâ‚‚HPOâ‚„, 1mM MgClâ‚‚ pH 7.2 in 20% Ethanol

4 to 30Â°C

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

2ËšC to 8ËšC, 20% ethanol

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% ethanol

2 to 8Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0

2Â°Câ€“8Â°C, 20% ethanol

2-8C

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2 to 8Â°C, 100% Isopropanol

2 to 8Â°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, 20% Ethanol, 0.05 M Sodium Acetate

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

2 to 8Â°C, 20% Ethanol

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

R-CODES

48/20

36/37/38

Surface Area

~500 m 2 /g

550 m 2 /g

175-225 m 2 /g

289 m 2 /g

480 m 2 /g

380 m 2 /g

500 m 2 /g

=480 m 2 /g (approx.)

430-530 m 2 /g

500 m 2 /g (approx.)

800 m 2 /g

250-350 m 2 /g

530 m 2 /g

300 m 2 /g

470-530 m 2 /g

~30 m 2 /g

RTECS Number

VV7315000

VV7310000

S-Codes

26-36

Pack Size

Packaging

Amber Glass

Percent Purity

≥99.9%

Residueafter Evaporation

5ppm max.

Sanitization Conditions

2% Hibitane/20% Ethanol or with 70% Ethanol

Stability

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stable

Signal Word

Danger

Warning

Scale

Lab|Process development

Lab|Process

Lab

Lab|Process development|Process

Purity

>=99%

99.8%

Storage Conditions

4 - 8Â°C, 20% Ethanol, 10% medium slurry

4ËšC to 30ËšC, 20% ethanol

4â€“30Â°C

20% ethanol at 4Â°C to 30Â°C

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Synonyms

Silica, fumed; Silica; Silicic anhydride; Silicon dioxide; Silicon dioxide amorphous

Silica gel 110 NH2

Amberlite(R) IR120 hydrogen form

Activated magnesium silicate; Magnesium silicate

Silica gel

Dowex(R) 1X8-200 Ion Exchange Resin

XAD16N

Stokes Radius

~ 10

IUPAC Name

methylsulfinylmethane

System

Sepax C-Pro|Sefia|Sepax 2

SMILES

CS(=O)C

Synonym

DMSO

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Strong anion exchanger (quaternary ammonium)

Theoretical Plates

> 43 000 m^-1

> 48 000 m^-1

> 42 000 m^-1

> 13000 m-1

5000 m-1

Titratable Acid

0.001mEq/g max.

WGK

nwg

>1600 C

Typical Pressure Drop Over the Packed Bed

~ 3 MPa

~ 2 MPa

Vapor Pressure

17 mmHg ( 20 °C)

0.55mbar at 20Â°C

Well Volume

8.5

Recommended Cleaning-in-Place Reagent

2 M NaCl solution

Vapor Density

>1 (vs air)

<1 (vs air)

Viscosity

1.98 mPa/s at 25Â°C

Viscosity Max

Wetted Part

Particle Size in Methanol Min

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Swelling factor

~1.7

Water

0.04% max.

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