Life Science > Proteomics > Protein Chemistry Reagents and Kits > Cell Lysis and Protein Extraction

Thermostable 5 App DNA/RNA Ligase - 10 reactions

by New England Biolabs

$95.04/EA

Average lead time: 5 to 7 Days

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Product Description

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Product Description

Thermostable 5' App DNA/RNA Ligase is a point mutant of catalytic lysine of RNA ligase from Methanobacterium thermoautotrophicum. This enzyme is ATP independent. It requires a 5' pre-adenylated linker for ligation to the 3' -OH end of either RNA or single stranded DNA (ssDNA). The enzyme is also active in ligation of RNA with 2' -O-methylated 3 end to 5-adenylated linkers. The optimal temperature for ligation reaction is 60-65°C. The mutant ligase is unable to adenylate the 5' -phosphate of RNA or ssDNA, which reduces the formation of undesired ligation products (concatemers and circles). The ability of the ligase to function at 65°C might reduce the constraints of RNA secondary structure in RNA ligation experiments.

Specifications

Inventory Number:

NEBM0319SEA

Part Number:

M0319S

Supplier:

New England Biolabs

Supplier's Lead Time:

5 to 7 Days

Quantity:

1 per EA

Contract Availability:

GSA

Country of Origin:

United States

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Thermostable 5 App DNA/RNA Ligase - 10 reactions

by New England Biolabs

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