Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) - 10 reactions

by New England Biolabs


  • E0552S | Q5 Site Directed Mutagenesis Kit Without Competent
  • E0552S | Q5 Site Directed Mutagenesis Kit Without Competent
Average lead time: 5 to 7 Days
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Product Description

The Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours). The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids. After PCR, the amplified material is added directly to a unique Kinase-Ligase-DpnI (KLD) enzyme mix for rapid (5 minutes), room temperature circularization and template removal. Transformation into high-efficiency chemically-competent E. coli, not supplied, ensures robust results with plasmids up to at least 20 kb in length. Kit is also available with competent cells .
Specifications
Inventory Number:
NEBE0552SEA
Part Number:
E0552S
Supplier:
New England Biolabs
Supplier's Lead Time:
5 to 7 Days
Quantity:
1 per EA
Contract Availability:
GSA
Country of Origin:
United States
Additional Information

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