Tissue grinders produce homogenates by a combination of shearing and compression actions. The tissue sample is progressively ground (sheared) into smaller pieces at the rounded end of the pestle as the spinning pestle is lowered into the tube. As the pestle is forced lower into the tube, the sample is displaced and forced between the straight outside wall of the pestle and the inside wall of the tube, compressing the tissue cells until they rupture. When the tube is pulled away from the pestle, a slight vacuum is created that pulls the sample back past the compression area, resulting in an additional homogenization stroke. The degree of homogenization is controlled by the clearance between the pestle’s and tube’s cylindrical section (radial distance usually 0.002 - 0.003 inches), the rotational speed of the pestle, and the number of compression strokes made.A Dounce tissue grinder''s pestle has a round, ball shape tip and is ddesigned for cellular studies where the nucleus needs to remain intact.
Features & Benefits
Manufactured from borosilicate glass 3.3
Designed primarily for cellular work where the nucleus remains intact after homogenization.
All-glass construction
Two pestles are supplied with each complete unit
Large clearance pestle is used for the initial sample reduction
Small clearance pestle is used to form the final homogenate
Replacement components are available and completely interchangeable