HisPrep FF 16/10, 20 mL
Optimized for convenient scaleup purification of histidinetagged proteins.
Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Negligible leakage of Ni2+.
High binding capacity, approx. 4NA mg/ml medium.
Convenient and timesaving 2NA ml prepacked HiPrep format.
HisPrep FF 16/1NA columns are prepacked with Ni Sepharose 6 Fast Flow for scaleup purification of histidinetagged proteins. Ni Sepharose 6 Fast Flow consists of 9NA ?m beads of highly crosslinked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the firstchoice metal ion for purifying most histidinetagged proteins. The negligible leakage of Ni2+ ions ensures reliable capture of histidinetagged proteins in repeated IMAC purifications.